BBa_K727005 1 BBa_K727005 Geranyl diphosphate Synthase 2012-06-15T11:00:00Z 2015-05-08T01:13:05Z Genom on GPPS (Geranyl diphosphate synthase) synthesizes GPP (Geranyl diphosphate) which is the first reaction of geraniol production. false false _973_ 0 11153 9 Not in stock true Optemized for E.coli false &#304;BRAH&#304;M YAS&#304;R ORHAN annotation2176259 1 GPPS range2176259 1 1 941 BBa_R0063 1 lux pL Promoter (luxR & HSL regulated -- lux pL)<br> 2003-01-31T12:00:00Z 2015-05-08T01:14:15Z <em>V. fischeri.</em> Released HQ 2013 The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl</p> false true _1_ 0 24 7 In stock false <P> <P> This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below.Includes most of Lux reulatory region, including the LuxR binding site which activates the right promoter. A putative LuxR autorepression binding site is also identified adjacent to the -10 site of the right promoter. This 2nd site has 55% identity with the first site. Putative inverted repeats (of size 18-27 bp) also exist between these two sites (not marked above), which may represent binding sites for other regulatory proteins. <p><img src="<bb_file>Image01.gif</bb_file>" width="614" height="362"><P>Unspecified. true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation2053 1 -35 range2053 1 89 94 annotation2055 1 Putative LuxR/HSL range2055 1 130 149 annotation7071 1 BBa_R0063 range7071 1 1 151 annotation2054 1 start range2054 1 128 128 annotation2052 1 -10 range2052 1 115 122 annotation2051 1 LuxR/HSL range2051 1 1 20 BBa_K727571 1 BBa_K727571 Rose Odor Generator Device (Geraniol) 2012-06-15T11:00:00Z 2015-05-08T01:13:05Z Genome of This part is assembled with Geranyl Diphosphate Synthase (http://partsregistry.org/wiki/index.php?title=Part:BBa_K727005) and Geraniol synthase (BBa_K727006)in order to produce geraniol. Geraniol is a monoterpenoid and an alcohol. It???s molecular formula is C10H18O . It is the primary part of rose oil, palmarosa oil, and citronella oil (Java type). It also occurs in small quantities in geranium, lemon, and many other essential oils. It has a rose-like odor and is commonly used in perfumes. It is used in flavors such as peach, raspberry, grapefruit, red apple, plum, lime, orange, lemon, watermelon, pineapple, and blueberry. true false _973_ 0 11153 9 Discontinued false Optimized for E.coli false &#304;BRAH&#304;M YAS&#304;R ORHAN component2176290 1 BBa_K727006 component2176288 1 BBa_K727005 component2176282 1 BBa_R0063 annotation2176290 1 BBa_K727006 range2176290 1 1109 2958 annotation2176282 1 BBa_R0063 range2176282 1 1 151 annotation2176288 1 BBa_K727005 range2176288 1 160 1100 BBa_K727006 1 BBa_K727006 Geraniol Synthase 2012-06-15T11:00:00Z 2015-05-08T01:13:05Z Genom on GS (Geraniol syntase) synthesizes Geraniol which is the second reaction of geraniol production. false false _973_ 0 11153 9 Not in stock true Optimized for E.coli false &#304;BRAH&#304;M YAS&#304;R ORHAN annotation2176260 1 GS range2176260 1 1 1850 BBa_R0063_sequence 1 acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaa BBa_K727005_sequence 1 gaattcgcggccgcttctagatggattttccgcagcagctggaagcatgtgttaaacaggcaaatcaggcactgagccgttttattgcaccgctgccgtttcagaataccccggttgttgaaaccatgcagtatggcgcactgctgggtggtaaacgtctgcgtccgtttctggtttatgcaaccggtcatatgtttggtgttagcaccaataccctggatgcaccggcagcagcagttgaatgtattcatgcatatagcctgattcatgatgatctgccggcaatggatgatgatgatctgcgtcgtggtctgccgacctgtcatgttaaatttggcgaagcaaatgcaattctggcaggtgatgcacttcagaccctggcatttagcattctgagcgatgcagatatgccggaagttagcgatcgtgatcgtattagcatgattagcgaactggcaagcgcaagcggtattgcaggtatgtgtggtggtcaggcactggatctggatgcagaaggtaaacatgttccgctggatgcactggaacgtattcatcgtcataaaaccggtgcactgattcgtgcagcagttcgtctgggtgcactgagcgcaggtgataaaggtcgtcgtgcactgccggttctggataaatatgcagaaagcattggtctggcatttcaggttcaggatgatattctggatgttgttggtgataccgcaaccctgggtaaacgtcagggtgcagatcagcagctgggtaaaagcacctatccggcactgctgggtctggaacaggcacgtaaaaaagcacaggatctgattgatgatgcacgtcagagcctgaaacagctggcagaacagagcattgataccagcgcactggaagcactggcagattatattattcagcgtaataaataatactagtagcggccgctgcag BBa_K727571_sequence 1 acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactagaggaattcgcggccgcttctagatggattttccgcagcagctggaagcatgtgttaaacaggcaaatcaggcactgagccgttttattgcaccgctgccgtttcagaataccccggttgttgaaaccatgcagtatggcgcactgctgggtggtaaacgtctgcgtccgtttctggtttatgcaaccggtcatatgtttggtgttagcaccaataccctggatgcaccggcagcagcagttgaatgtattcatgcatatagcctgattcatgatgatctgccggcaatggatgatgatgatctgcgtcgtggtctgccgacctgtcatgttaaatttggcgaagcaaatgcaattctggcaggtgatgcacttcagaccctggcatttagcattctgagcgatgcagatatgccggaagttagcgatcgtgatcgtattagcatgattagcgaactggcaagcgcaagcggtattgcaggtatgtgtggtggtcaggcactggatctggatgcagaaggtaaacatgttccgctggatgcactggaacgtattcatcgtcataaaaccggtgcactgattcgtgcagcagttcgtctgggtgcactgagcgcaggtgataaaggtcgtcgtgcactgccggttctggataaatatgcagaaagcattggtctggcatttcaggttcaggatgatattctggatgttgttggtgataccgcaaccctgggtaaacgtcagggtgcagatcagcagctgggtaaaagcacctatccggcactgctgggtctggaacaggcacgtaaaaaagcacaggatctgattgatgatgcacgtcagagcctgaaacagctggcagaacagagcattgataccagcgcactggaagcactggcagattatattattcagcgtaataaataatactagtagcggccgctgcagtactagaggaattcgcggccgcttctagatgagcagcattagccagaaagttgttattggtctgaataaagcagcagcaaataataatttacagaatctggatcgtcgtggttttaaaacccgttgtgttagcagcagcaaagcagcaagctgtctgcgtgcaagctgtagcttacagctggatgttaaaccggttcaggaaggtcgtcgtagcggtaattatcagccgagcatttgggattttaattatgttcagagcctgaataccccgtataaagaagaacgttatctgacccgtcatgcagaactgattgttcaggttaaaccgctgctggaaaaaaaaatggaaccggcacagcagctggaactgattgatgatctgaataatctgggtctgagctatttttttcaggatcgtattaaacagattctgagctttatttatgatgaaaatcagtgttttcatagcaatattaatgatcaggcagaaaaacgtgatctgtattttaccgcactgggttttcgtctgctgcgtcagcatggttttgatgttagccaggaagtttttgattgttttaaaaatgataatggtagcgattttaaagcaagcctgagcgataataccaaaggtctgttacagctgtatgaagcaagctttctggttcgtgaaggtgaagataccctggaacaggcacgtcagtttgcaaccaagtttctgcgtcgtaaactggatgaaattgatgataatcatctgctgagctgtattcatcatagcctggagattccgctgcattggcgtattcagcgtctggaagcacgttggtttctggatgcatatgcaacccgtcatgatatgaatccggttattctggaactggcaaaactggattttaatattattcaggcaacccatcaggaagaactgaaagatgttagccgttggtggcagaatacccgtctggcagaaaaactgccgtttgttcgtgatcgtctggttgaaagctatttttgggcaattgcactgtttgaaccgcatcagtatggttatcagcgtcgtgttgcagcaaaaattattaccctggcaaccagcattgatgatgtttatgatatttatggtaccctggatgaattacagctgtttaccgataattttcgtcgttgggataccgaaagcctgggtcgtctgccgtatagcatgcagctgttttatatggttattcataattttgttagcgaactggcatatgagattctgaaagaaaaaggttttattgttattccgtatttacagcgtagctgggttgatctggcagaaagctttctgaaagaagcaaattggtattatagcggttataccccgagcctggaagaatatattgataatggtagcattagcattggtgcagttgcagttctgagccaggtttattttaccctggcaaatagcattgaaaaaccgaaaattgaaagcatgtataaatatcatcatattctgcgtctgagcggtctgctggttcgtctgcatgatgatctgggtaccagcctgtttgaaaaaaaacgtggtgatgttccgaaagcagttgagatttgtatgaaagaacgtaatgttaccgaagaagaagcagaagaacatgttaaatatctgattcgtgaagcatggaaagaaatgaataccgcaaccaccgcagcaggttgtccgtttatggatgaactgaatgttgcagcagcaaatctgggtcgtgcagcacagtttgtttatctggatggtgatggtcatggtgttcagcatagcaagattcatcagcagatgggtggtctgatgtttgaaccgtatgtttactagtagcggccgctgcag BBa_K727006_sequence 1 gaattcgcggccgcttctagatgagcagcattagccagaaagttgttattggtctgaataaagcagcagcaaataataatttacagaatctggatcgtcgtggttttaaaacccgttgtgttagcagcagcaaagcagcaagctgtctgcgtgcaagctgtagcttacagctggatgttaaaccggttcaggaaggtcgtcgtagcggtaattatcagccgagcatttgggattttaattatgttcagagcctgaataccccgtataaagaagaacgttatctgacccgtcatgcagaactgattgttcaggttaaaccgctgctggaaaaaaaaatggaaccggcacagcagctggaactgattgatgatctgaataatctgggtctgagctatttttttcaggatcgtattaaacagattctgagctttatttatgatgaaaatcagtgttttcatagcaatattaatgatcaggcagaaaaacgtgatctgtattttaccgcactgggttttcgtctgctgcgtcagcatggttttgatgttagccaggaagtttttgattgttttaaaaatgataatggtagcgattttaaagcaagcctgagcgataataccaaaggtctgttacagctgtatgaagcaagctttctggttcgtgaaggtgaagataccctggaacaggcacgtcagtttgcaaccaagtttctgcgtcgtaaactggatgaaattgatgataatcatctgctgagctgtattcatcatagcctggagattccgctgcattggcgtattcagcgtctggaagcacgttggtttctggatgcatatgcaacccgtcatgatatgaatccggttattctggaactggcaaaactggattttaatattattcaggcaacccatcaggaagaactgaaagatgttagccgttggtggcagaatacccgtctggcagaaaaactgccgtttgttcgtgatcgtctggttgaaagctatttttgggcaattgcactgtttgaaccgcatcagtatggttatcagcgtcgtgttgcagcaaaaattattaccctggcaaccagcattgatgatgtttatgatatttatggtaccctggatgaattacagctgtttaccgataattttcgtcgttgggataccgaaagcctgggtcgtctgccgtatagcatgcagctgttttatatggttattcataattttgttagcgaactggcatatgagattctgaaagaaaaaggttttattgttattccgtatttacagcgtagctgggttgatctggcagaaagctttctgaaagaagcaaattggtattatagcggttataccccgagcctggaagaatatattgataatggtagcattagcattggtgcagttgcagttctgagccaggtttattttaccctggcaaatagcattgaaaaaccgaaaattgaaagcatgtataaatatcatcatattctgcgtctgagcggtctgctggttcgtctgcatgatgatctgggtaccagcctgtttgaaaaaaaacgtggtgatgttccgaaagcagttgagatttgtatgaaagaacgtaatgttaccgaagaagaagcagaagaacatgttaaatatctgattcgtgaagcatggaaagaaatgaataccgcaaccaccgcagcaggttgtccgtttatggatgaactgaatgttgcagcagcaaatctgggtcgtgcagcacagtttgtttatctggatggtgatggtcatggtgttcagcatagcaagattcatcagcagatgggtggtctgatgtttgaaccgtatgtttactagtagcggccgctgcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z