##gff-version 3
##sequence-region BBa_K727700 1 3273
BBa_K727700	.	stem_loop	952	995	.	+	0	ID=annotation2176832;Name=stem_loop
BBa_K727700	.	engineered_region	227	907	.	+	0	ID=annotation2176828;Name=BBa_E1010
BBa_K727700	.	CDS	227	901	.	+	0	ID=annotation2176829;Name=mrfp1
BBa_K727700	.	stem_loop	1036	1055	.	+	0	ID=annotation2176834;Name=T7 TE
BBa_K727700	.	conserved_region	213	216	.	+	0	ID=annotation2176827;Name=conserved
BBa_K727700	.	start_codon	173	173	.	+	0	ID=annotation2176825;Name=start
BBa_K727700	.	stem_loop	1079	3273	.	+	0	ID=annotation2176839;Name=pSB1K3
BBa_K727700	.	polyA_site	1056	1069	.	+	0	ID=annotation2176835;Name=polya
BBa_K727700	.	engineered_tag	908	932	.	+	0	ID=annotation2176830;Name=barcode
BBa_K727700	.	promoter	137	142	.	+	0	ID=annotation2176822;Name=-35
BBa_K727700	.	stop_codon	1062	1062	.	+	0	ID=annotation2176836;Name=stop
BBa_K727700	.	engineered_region	1	1069	.	+	0	ID=annotation2176837;Name=BBa_J04450
BBa_K727700	.	engineered_region	941	1020	.	+	0	ID=annotation2176831;Name=BBa_B0010
BBa_K727700	.	engineered_region	1029	1069	.	+	0	ID=annotation2176833;Name=BBa_B0012
BBa_K727700	.	engineered_region	209	220	.	+	0	ID=annotation2176826;Name=BBa_B0034
BBa_K727700	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation2176821;Name=CAP
BBa_K727700	.	engineered_region	1	200	.	+	0	ID=annotation2176820;Name=BBa_R0010
BBa_K727700	.	promoter	161	166	.	+	0	ID=annotation2176823;Name=-10
BBa_K727700	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation2176824;Name=LacI
>BBa_K727700
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctc
cgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaa
ggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcg
cttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacct
gaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggtta
tgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaa
aatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcag
ctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacg
aacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagtagcggccgctgcagtccggcaaaaaagggc
aaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgact
cgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcagg
ggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgttt
ttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactat
aaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtc
cgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgc
tccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtcca
acccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgc
tacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagcca
gttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgca
agcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaa
cgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatga
agttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgagtcccgtcaagtcagcgtaatgctctg
ccagtgttacaaccaattaaccaattctgattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatca
ggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatgg
caagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataag
gttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagact
tgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcct
gagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgc
cagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtg
gtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagttta
gtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggctt
cccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatcc
atgttggaatttaatcgcggcctggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgttta
tgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaac
gtggctttgttgaataaatcgaacttttgctgagttgaaggatcagctcgagtgccacctgacgtctaagaaaccatta
ttatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaa
ggatgatttctggaattcgcggccgcttctagag