##gff-version 3
##sequence-region BBa_K727777 1 3111
BBa_K727777	.	engineered_region	64	1700	.	+	0	ID=BBa_K727004;Name=BBa_K727004
BBa_K727777	.	CDS	64	1700	.	+	0	ID=annotation2176258;Name=FMO3;Parent=BBa_K727004
BBa_K727777	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K727777	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K727777	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K727777	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K727777	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K727777	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K727777	.	CDS	2494	3111	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K727777	.	CDS	2494	3072	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K727777	.	engineered_tag	3112	3136	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K727777	.	tag	3073	3104	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K727777	.	engineered_region	2494	3111	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K727777	.	CDS	1707	2462	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K727777	.	CDS	1707	2456	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K727777	.	engineered_region	1707	2462	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K727777	.	engineered_region	1707	1708	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K727777	.	point_mutation	2198	2198	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K727777	.	engineered_tag	2463	2487	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K727777	.	point_mutation	1880	1880	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
>BBa_K727777
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagaggaattcgcggccgctt
ctagatgggtaaaaaagttgcaattattggtgcaggtgttagcggtctggcaagcattcgtagctgtctggaagaaggt
ctggaaccgacctgttttgaaaaaagcaatgatattggtggtctgtggaaatttagcgatcatgcagaagaaggtcgtg
caagcatttataaaagcgtttttagcaatagcagcaaagaaatgatgtgttttccggattttccgtttccggatgattt
tccgaattttatgcataatagcaaaattcaggaatatattattgcatttgcaaaagaaaaaaatctgctgaaatatatt
cagtttaaaacctttgttagcagcgttaataaacatccggattttgcaaccaccggtcagtgggatgttaccaccgaac
gtgatggtaaaaaagaaagcgcagtttttgatgcagttatggtttgtagcggtcatcatgtttatccgaatctgccgaa
agaaagctttccgggtctgaatcattttaaaggtaaatgttttcatagccgtgattataaagaaccgggtgtttttaat
ggtaaacgtgttctggttgttggtctgggtaatagcggttgtgatattgcaaccgaactgagccgtaccgcagaacagg
ttatgattagcagccgtagcggtagctgggttatgagccgtgtttgggataatggttatccgtgggatatgctgctggt
tacccgttttggtacctttctgaaaaataatctgccgaccgcaattagcgattggctgtatgttaaacagatgaatgca
cgttttaaacatgaaaattatggtctgatgccgctgaatggtgttctgcgtaaagaaccggtttttaatgatgaactgc
cggcaagcattctgtgtggtattgttagcgttaaaccgaatgttaaagaatttaccgaaaccagcgcaatttttgaaga
tggtaccatttttgaaggtattgattgtgttatttttgcaaccggttatagctttgcatatccgtttctggatgaaagc
attattaaaagccgtaataatgaaattattctgtttaaaggtgtttttccgccgctgctggaaaaaagcaccattgcag
ttattggttttgttcagagcctgggtgcagcaattccgaccgttgatctgcagagccgttgggcagcacaggttattaa
aggtacctgtaccctgccgagcatggaagatatgatgaatgatattaatgaaaaaatggaaaaaaaacgtaaatggttt
ggtaaaagcgaaaccattcagaccgattatattgtttatatggatgaactgagcagctttattggtgcaaaaccgaata
ttccgtggctgtttctgaccgatccgaaactggcaatggaagtttattttggtccgtgtagcccgtatcagtttcgtct
ggttggtccgggtcagtggccgggtgcacgtaatgcaattctgacccagtgggatcgtagcctgaaaccgatgcagacc
cgtgttgttggtcgtctgcagaaaccgtgttttttttttcattggctgaaactgtttgcaattccgattctgctgattg
cagtttttctggttctgacctactagtagcggccgctgcagtactagatgaaaaacataaatgccgacgacacatacag
aataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacat
tgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattacccta
aaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattc
accaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatca
ggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaa
aagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataatta
tcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgc
gaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgc
aaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactt
taaaaattaataacactgatagtgctagtgtagatcactactagatgactataatgataaaaaaatcggattttttggc
aattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagtt
gtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatg
taagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaaca
gagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactct
gctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacag
taacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgt
attaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaac
gacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc