LOCUS BBa_K729000 7461 bp DNA linear 22-Nov-2024 DEFINITION Temperature regulated Gas Vesicle Polycistronic gene cluster composite ACCESSION BBa_K729000 VERSION BBa_K729000.1 FEATURES Location/Qualifiers promoter 1..98 /label=BBa_K338001 misc_feature 111..114 /label=conserved RBS 107..118 /label=BBa_B0034 misc_feature 125..127 /label=start misc_feature 1001..1006 /label=stop CDS 125..1006 /label=DapA CDS 125..1006 /label=BBa_I718004 stem_loop 1026..1069 /label=stem_loop misc_feature 1015..1094 /label=BBa_B0010 terminator 1015..1094 /label=BBa_B0010 stem_loop 1110..1129 /label=T7 TE misc_feature 1136..1136 /label=stop polyA_site 1130..1143 /label=polya misc_feature 1103..1143 /label=BBa_B0012 terminator 1103..1143 /label=BBa_B0012 terminator 1015..1143 /label=BBa_B0015 promoter 1152..1232 /label=BBa_I718018 misc_feature 1245..1248 /label=conserved RBS 1241..1252 /label=BBa_B0034 RBS 1261..1272 /label=b0034 CDS 1279..1545 /label=gvpB CDS 1621..1887 /label=gvpR CDS 1916..2843 /label=gvpN CDS 2857..3624 /label=gvpF CDS 3624..3892 /label=gvpG CDS 3893..5102 /label=gvpL CDS 5120..5407 /label=gvpS CDS 5361..5645 /label=gvpK CDS 5667..5969 /label=gvpJ CDS 6006..6884 /label=gvpT CDS 6908..7324 /label=gvpU misc_feature 4105..4105 /label=gvpL g213a misc_feature 4210..4210 /label=gvpL g318a misc_feature 4243..4243 /label=gvpL g351a misc_feature 4588..4588 /label=gvpL g696a misc_feature 4604..5003 /label=10 repeats of 40bp induced durring mutagenesis misc_feature 1978..1978 /label=c->a misc_feature 3963..3963 /label=t->c misc_feature 4032..4032 /label=t->c misc_feature 4482..4482 /label=t->a misc_feature 5792..5792 /label=t->a misc_feature 6330..6330 /label=g->a misc_feature 6393..6393 /label=g->a misc_feature 4564..4603 /label=First copy of 40bp section misc_feature 1261..7324 /label=BBa_I750016 stem_loop 7344..7387 /label=stem_loop misc_feature 7333..7412 /label=BBa_B0010 terminator 7333..7412 /label=BBa_B0010 stem_loop 7428..7447 /label=T7 TE misc_feature 7454..7454 /label=stop polyA_site 7448..7461 /label=polya misc_feature 7421..7461 /label=BBa_B0012 terminator 7421..7461 /label=BBa_B0012 terminator 7333..7461 /label=BBa_B0015 ORIGIN 1 ccgaggtcct tgttgcgaag attgatgaca atgtgagtgc ttcccttgaa accctgaaac 61 tgatccccat aataagcgaa gttagcgaga tgaatgcgta ctagagaaag aggagaaata 121 ctagatgttc acgggaagta ttgtcgcgat tgttactccg atggatgaaa aaggtaatgt 181 ctgtcgggct agcttgaaaa aactgattga ttatcatgtc gccagcggta cttcggcgat 241 cgtttctgtt ggcaccactg gcgagtccgc taccttaaat catgacgaac atgctgatgt 301 ggtgatgatg acgctggatc tggctgatgg gcgcattccg gtaattgccg ggaccggcgc 361 taacgctact gcggaagcca ttagcctgac gcagcgcttc aatgacagtg gtatcgtcgg 421 ctgcctgacg gtaacccctt actacaatcg tccgtcgcaa gaaggtttgt atcagcattt 481 caaagccatc gctgagcata ctgacctgcc gcaaattctg tataatgtgc cgtcccgtac 541 tggctgcgat ctgctcccgg aaacggtggg ccgtctggcg aaagtaaaaa atattatcgg 601 aatcaaagag gcaacaggga acttaacgcg tgtaaaccag atcaaagagc tggtttcaga 661 tgattttgtt ctgctgagcg gcgatgatgc gagcgcgctg gacttcatgc aattgggcgg 721 tcatggggtt atttccgtta cggctaacgt cgcagcgcgt gatatggccc agatgtgcaa 781 actggcagca gaagggcatt ttgccgaggc acgcgttatt aatcagcgtc tgatgccatt 841 acacaacaaa ctatttgtcg aacccaatcc aatcccggtg aaatgggcat gtaaggaact 901 gggtcttgtg gcgaccgata cgctgcgcct gccaatgaca ccaatcaccg acagtggtcg 961 tgagacggtc agagcggcgc ttaagcatgc cggtttgctg taataatact agagccaggc 1021 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 1081 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 1141 atatactaga gaacaatcag aacggttctg tctgcttgct tttaatgcca taccaaacgt 1201 accattgaga cacttgtttg cacagaggat ggtactagag aaagaggaga aatactagag 1261 aaagaggaga aatactagat gtctattcaa aaaagtacta atagttcaag tttagcagaa 1321 gtcattgacc gtattttaga taaaggaatt gttattgatg cttttgcaag agtttctgtt 1381 gtaggaattg aaattttaac gattgaagcg cgagtggtta ttgccagtgt tgatacatgg 1441 ttacgctatg cagaagcagt agggcttctt cgtgacgacg tagaagaaaa cggtcttcct 1501 gaacgttcaa attcaagtga agggcagccg cgttttagta tttaatatga agtggtatgg 1561 aaccttcctt ttttgagggt ggttccagct tttagatcta actattggag gctactaaaa 1621 atggaaatta aaaaaattat gcaagccgtg aacgactttt tcggtgaaca cgtagctcct 1681 cctcataaaa ttacctcggt ggaagctact gaagatgaag gttggagagt tattgttgaa 1741 gtcattgaag aacgagaata tatgaaaaaa tacgccaaag atgaaatgct cggaacgtac 1801 gagtgctttg taaataaaga aaaagaagtc atttcattca aacgactcga cgtcagatat 1861 agaagcgcca ttggcattga agcataaacg ataagatggc aggaggaacg taaaaatgac 1921 cgtcttaaca gacaaaagga aaaaaggcag tggagctttt atacaagatg acgagacaaa 1981 agaggttctt tcaagagcgc tgagctattt aaaatccggc tattccattc attttacagg 2041 tcctgccggc ggaggcaaaa cctctttagc gcgagcgctt gctaaaaaga gaaagcgtcc 2101 tgtaatgctg atgcacggga atcacgagct caacaacaaa gatttaattg gcgattttac 2161 gggatacacg agcaaaaaag taatcgacca gtacgttcgt tctgtctata aaaaagatga 2221 acaggtgagt gaaaactggc aggatggccg attgcttgaa gctgtaaaaa atggctatac 2281 gctgatttac gacgaattta ctcgttctaa gcctgcgacg aataatatct ttctatcgat 2341 attagaagaa ggcgtgctgc cgctgtatgg agtaaaaatg accgatcctt ttgtgcgcgt 2401 gcatcccgat ttccgcgtca tcttcacaag caatccagct gagtatgccg gcgtatatga 2461 tacgcaagat gcgcttctcg acaggttaat taccatgttt attgattata aagacatcga 2521 cagagagaca gcgattttaa cggagaaaac ggacgtagaa gaagatgaag cgcgcacaat 2581 tgtaacgctc gtagcaaacg tgcgaaaccg ctctggagac gaaaacagca gcggacttag 2641 cctgcgggct tcgcttatga tcgctaccct tgccacgcag caagacattc ctatcgatgg 2701 aagtgacgaa gattttcaaa cgttatgtat cgatattttg catcatccgc ttaccaaatg 2761 tttggatgaa gaaaatgcaa aaagcaaagc cgaaaaaatc attttagaag aatgtaagaa 2821 tatagacact gaagaaaagt aaaggagctt gaaaacatga gtgaaacaaa cgaaacaggt 2881 atttatattt ttagcgccat tcaaacggat aaagacgaag aatttggcgc cgtggaagta 2941 gaaggaacaa aagctgaaac atttttgatt cgctacaaag acgcggctat ggtagcagct 3001 gaagtaccga tgaaaattta tcatcctaat cgccaaaatt tattaatgca tcaaaacgca 3061 gtagcagcga ttatggacaa gaacgatacg gttattccaa tcagctttgg gaatgtattc 3121 aaatcaaaag aagacgtaaa agttcttttg gaaaaccttt atccgcagtt tgaaaagctg 3181 tttccagcga tcaaaggaaa aattgaagtc ggtttaaaag taattgggaa aaaagaatgg 3241 cttgagaaaa aagtaaacga aaatcctgaa cttgagaaag tatcagcatc cgtaaaagga 3301 aaatcagaag cagccggtta ttatgagcgt attcaacttg gaggaatggc tcaaaagatg 3361 tttacttccc tgcaaaaaga agtcaagaca gatgtgtttt ctccgcttga agaagcagcg 3421 gaagcagcaa aagcaaatga gccaacgggc gaaacgatgc ttttaaacgc gtctttctta 3481 attaaccgag aagatgaagc gaagtttgat gaaaaagtaa atgaagcgca tgaaaactgg 3541 aaagacaaag ccgattttca ttacagcggt ccttggcctg cttataattt tgtgaacatt 3601 cgcctaaaag tagaagagaa ataacgtgct tcacaaatta gtaaccgcac ccattaacct 3661 tgtagtgaaa atcggcgaaa aagtacagga agaagctgat aaacagctat atgaccttcc 3721 gacgattcag caaaagctca ttcagcttca aatgatgttt gagcttggtg aaattccaga 3781 agaagcgttt caagaaaaag aagatgaatt gttaatgagg tacgaaattg cgaaacgcag 3841 agaaattgaa caatgggaag agctaacaca aaaaagaaat gaggaatcct agatgggaga 3901 attactgtat ttatacggtt taattccaac aaaagaagca gcagccatag agccgtttcc 3961 atcttataag gggtttgacg gagaacattc actgtaccca attgcgtttg atcaggtgac 4021 ggctgtagtt tctaagctgg atgctgacac ctattcagaa aaagtgattc aagaaaaaat 4081 ggagcaggat atgagctggc tgcaagaaaa agcatttcat catcacgaaa cggtagccgc 4141 tttgtacgaa gaatttacga tcattccatt aaaattttgc accatttata aaggtgaaga 4201 aagtctgcaa gcagctattg agattaacaa agaaaagata gaaaattcac tgacgctgct 4261 tcaaggaaat gaagagtgga atgtgaaaat ttactgtgat gatacagagc ttaaaaaagg 4321 aatcagcgaa acgaatgaaa gcgtgaaagc gaaaaaacaa gaaattagtc acttatcacc 4381 aggaagacag ttttttgaaa agaaaaaaat agatcagctg attgaaaaag aattagagct 4441 tcacaaaaac aaagtgtgtg aagagataca tgacaagcta aaagaattat cgctttatga 4501 ctctgttaaa aagaattgga gcaaagacgt aactggcgca gctgaacaga tggcgtggaa 4561 cagcgtgttt cttctcccgt ctctgcaaat tactaagttc gtacgtgttt cttctcccgt 4621 ctctgcaaat tactaagttc gtacgtgttt cttctcccgt ctctgcaaat tactaagttc 4681 gtacgtgttt cttctcccgt ctctgcaaat tactaagttc gtacgtgttt cttctcccgt 4741 ctctgcaaat tactaagttc gtacgtgttt cttctcccgt ctctgcaaat tactaagttc 4801 gtacgtgttt cttctcccgt ctctgcaaat tactaagttc gtacgtgttt cttctcccgt 4861 ctctgcaaat tactaagttc gtacgtgttt cttctcccgt ctctgcaaat tactaagttc 4921 gtacgtgttt cttctcccgt ctctgcaaat tactaagttc gtacgtgttt cttctcccgt 4981 ctctgcaaat tactaagttc gtaaacgaaa tagaagagct tcagcaaagg cttgaaaata 5041 aaggctggaa gtttgaagtg acgggaccat ggccgcccta tcatttctcg agctttgcgt 5101 aaagtgagga attaacatta tgtctcttaa acaatccatg gagaataaag atattgctct 5161 tattgatatt ttagatgtca ttttagataa aggagtcgcc attaaaggag acttaatcat 5221 ttccatagct ggcgtcgatt tagtgtattt ggatttgcgg gtgcttattt cttcggttga 5281 aacgcttgtg caagcaaaag aaggaaatca caaaccaatc acttctgaac aatttgataa 5341 acaaaaggag gaattaatgg atgcaaccgg tcagccaagc aaatggacga atccacttgg 5401 atcctgatca agctgaacaa ggcttagcgc agcttgtgat gacagttatt gagctattga 5461 ggcaaatcgt tgaacgtcat gccatgaggc gggtggaggg tggaacgttg acggacgaac 5521 aaattgaaaa cttaggaatt gcactaatga acttagaaga aaaaatggac gagttgaaag 5581 aggtgttcgg tctggatgca gaagatttaa atattgatct tggaccgcta ggcagcctgc 5641 tttaagcggt cagtaggagg aacagtatgg cagtcgaaca taatatgcag tcaagtacga 5701 ttgtagatgt gctcgaaaag attttggata aaggagtcgt tatagcgggg gacatcaccg 5761 taggaattgc agatgtcgag ctattaacga taaagatccg cttgattgtg gcttcggttg 5821 ataaggcaaa agaaatcggc atggactggt gggaaaatga tccgtatctc agttcaaaag 5881 gagccaataa caaagcgctc gaagaagaaa ataaaatgct gcatgagcgg ttaaaaacgc 5941 ttgaagaaaa aatagaaacg aaacgttaaa aactgtacgc tacttaaaaa atggagggat 6001 ttacaatggc aactgaaaca aaattagata acacacaggc agaaaacaag gaaaataaaa 6061 atgcggaaaa cggttcaaaa gaaaagaacg gttcaaaagc aagcaaaaca acaagcagcg 6121 ggccaatcaa acgagcggta gcaggaggca tcatcggtgc aacgattgga tatgtatcga 6181 ctcctgaaaa tcgaaaaagt ctccttgacc gcattgatac agacgaatta aaaagcaaag 6241 catctgattt aggaacaaag gtaaaagaaa aatcaaaaag cagcgtggcc agcctgaaaa 6301 catctgcggg aagcttgttt aaaaaagata aagataaatc aaaagatgat gaagaaaacg 6361 taaattcttc tagtagcgaa acagaagacg ataacgttca agagtacgac gagttaaaag 6421 aagaaaatca aactcttcaa gatcgcttat cacagcttga agaaaaaatg aacatgcttg 6481 ttgagcttag cctcaataaa aatcaagacg aagaagcgga agatacagat tccgacgaag 6541 aagagaacga tgagaacgat gaaaacgatg aaaacgagca ggacgatgaa aacgaagaag 6601 aaacatctaa gccacgtaaa aaggataaaa aagaagctga ggaagaagaa agtgaaagtg 6661 acgaagacag cgaggaagaa gaggaagatt ctcgctcaaa caaaaaaaat aaaaaagtaa 6721 aaacagaaga agaagacgaa gatgaaagcg aagaagaaaa aaaggaagcg aaaccaaaaa 6781 agtcaacagc taaaaaatca aaaaatacaa aagcaaagaa aaacacggac gaagaagatg 6841 atgaagcaac atctctttct agtgaagacg atacaacagc ctaagacgta aaggaggaaa 6901 gaaagacatg agtacaggcc cttctttttc aactaaagac aatacgcttg aatactttgt 6961 gaaagcttct aataaacacg gcttttcact tgatatttca ttaaatgtaa acggcgctgt 7021 gatttccggt accatgattt cagcaaaaga atactttgat tacttaagcg aaacgtttga 7081 agaaggcagt gaagtggctc aggcgctaag cgaacaattc tctttagcaa gcgaagcgag 7141 cgaatcaaac ggagaagcag aagcccattt tattcatttg aaaaatacaa agatttactg 7201 tggagacagt aaatctactc cttctaaagg caaaatcttt tggagaggga aaatagcaga 7261 agtagacggg tttttcttag gaaagatttc tgatgcaaaa tcaacgagta aaaagagttc 7321 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 7381 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 7441 gtgggccttt ctgcgtttat a //