##gff-version 3
##sequence-region BBa_K729000 1 7461
BBa_K729000	.	terminator	7333	7461	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K729000	.	terminator	7333	7412	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K729000	.	stem_loop	7344	7387	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K729000	.	engineered_region	7333	7412	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K729000	.	terminator	7421	7461	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K729000	.	engineered_region	7421	7461	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K729000	.	stop_codon	7454	7454	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K729000	.	stem_loop	7428	7447	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K729000	.	polyA_site	7448	7461	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K729000	.	ribosome_entry_site	107	118	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K729000	.	conserved_region	111	114	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K729000	.	promoter	1152	1232	.	+	0	ID=BBa_I718018;Name=BBa_I718018
BBa_K729000	.	promoter	1	98	.	+	0	ID=BBa_K338001;Name=BBa_K338001
BBa_K729000	.	ribosome_entry_site	1241	1252	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K729000	.	conserved_region	1245	1248	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K729000	.	engineered_region	1261	7324	.	+	0	ID=BBa_I750016;Name=BBa_I750016
BBa_K729000	.	point_mutation	6393	6393	.	+	0	ID=annotation1954605;Name=g->a;Parent=BBa_I750016
BBa_K729000	.	sequence_feature	4604	5003	.	+	0	ID=annotation1954598;Name=10 repeats of 40bp induced durring mutagenesis;Parent=BBa_I750016
BBa_K729000	.	CDS	6908	7324	.	+	0	ID=annotation1951693;Name=gvpU;Parent=BBa_I750016
BBa_K729000	.	CDS	1279	1545	.	+	0	ID=annotation1951683;Name=gvpB;Parent=BBa_I750016
BBa_K729000	.	sequence_alteration	4482	4482	.	+	0	ID=annotation1954602;Name=t->a;Parent=BBa_I750016
BBa_K729000	.	CDS	1916	2843	.	+	0	ID=annotation1951685;Name=gvpN;Parent=BBa_I750016
BBa_K729000	.	CDS	5361	5645	.	+	0	ID=annotation1951690;Name=gvpK;Parent=BBa_I750016
BBa_K729000	.	CDS	5667	5969	.	+	0	ID=annotation1951691;Name=gvpJ;Parent=BBa_I750016
BBa_K729000	.	point_mutation	6330	6330	.	+	0	ID=annotation1954604;Name=g->a;Parent=BBa_I750016
BBa_K729000	.	point_mutation	4105	4105	.	+	0	ID=annotation1951694;Name=gvpL g213a;Parent=BBa_I750016
BBa_K729000	.	point_mutation	4032	4032	.	+	0	ID=annotation1954601;Name=t->c;Parent=BBa_I750016
BBa_K729000	.	point_mutation	4243	4243	.	+	0	ID=annotation1951697;Name=gvpL g351a;Parent=BBa_I750016
BBa_K729000	.	CDS	1621	1887	.	+	0	ID=annotation1951684;Name=gvpR;Parent=BBa_I750016
BBa_K729000	.	CDS	3624	3892	.	+	0	ID=annotation1951687;Name=gvpG;Parent=BBa_I750016
BBa_K729000	.	point_mutation	1978	1978	.	+	0	ID=annotation1954599;Name=c->a;Parent=BBa_I750016
BBa_K729000	.	CDS	2857	3624	.	+	0	ID=annotation1951686;Name=gvpF;Parent=BBa_I750016
BBa_K729000	.	point_mutation	5792	5792	.	+	0	ID=annotation1954603;Name=t->a;Parent=BBa_I750016
BBa_K729000	.	CDS	6006	6884	.	+	0	ID=annotation1951692;Name=gvpT;Parent=BBa_I750016
BBa_K729000	.	CDS	3893	5102	.	+	0	ID=annotation1951688;Name=gvpL;Parent=BBa_I750016
BBa_K729000	.	point_mutation	4588	4588	.	+	0	ID=annotation1951698;Name=gvpL g696a;Parent=BBa_I750016
BBa_K729000	.	CDS	5120	5407	.	+	0	ID=annotation1951689;Name=gvpS;Parent=BBa_I750016
BBa_K729000	.	point_mutation	3963	3963	.	+	0	ID=annotation1954600;Name=t->c;Parent=BBa_I750016
BBa_K729000	.	sequence_feature	4564	4603	.	+	0	ID=annotation1954616;Name=First copy of 40bp section;Parent=BBa_I750016
BBa_K729000	.	point_mutation	4210	4210	.	+	0	ID=annotation1951696;Name=gvpL g318a;Parent=BBa_I750016
BBa_K729000	.	ribosome_entry_site	1261	1272	.	+	0	ID=annotation1951682;Name=b0034;Parent=BBa_I750016
BBa_K729000	.	terminator	1015	1143	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K729000	.	terminator	1015	1094	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K729000	.	stem_loop	1026	1069	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K729000	.	engineered_region	1015	1094	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K729000	.	terminator	1103	1143	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K729000	.	engineered_region	1103	1143	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K729000	.	stop_codon	1136	1136	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K729000	.	stem_loop	1110	1129	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K729000	.	polyA_site	1130	1143	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K729000	.	CDS	125	1006	.	+	0	ID=BBa_I718004;Name=BBa_I718004
BBa_K729000	.	stop_codon	1001	1006	.	+	0	ID=annotation1939202;Name=stop;Parent=BBa_I718004
BBa_K729000	.	CDS	125	1006	.	+	0	ID=annotation1939203;Name=DapA;Parent=BBa_I718004
BBa_K729000	.	start_codon	125	127	.	+	0	ID=annotation1939201;Name=start;Parent=BBa_I718004
>BBa_K729000
ccgaggtccttgttgcgaagattgatgacaatgtgagtgcttcccttgaaaccctgaaactgatccccataataagcga
agttagcgagatgaatgcgtactagagaaagaggagaaatactagatgttcacgggaagtattgtcgcgattgttactc
cgatggatgaaaaaggtaatgtctgtcgggctagcttgaaaaaactgattgattatcatgtcgccagcggtacttcggc
gatcgtttctgttggcaccactggcgagtccgctaccttaaatcatgacgaacatgctgatgtggtgatgatgacgctg
gatctggctgatgggcgcattccggtaattgccgggaccggcgctaacgctactgcggaagccattagcctgacgcagc
gcttcaatgacagtggtatcgtcggctgcctgacggtaaccccttactacaatcgtccgtcgcaagaaggtttgtatca
gcatttcaaagccatcgctgagcatactgacctgccgcaaattctgtataatgtgccgtcccgtactggctgcgatctg
ctcccggaaacggtgggccgtctggcgaaagtaaaaaatattatcggaatcaaagaggcaacagggaacttaacgcgtg
taaaccagatcaaagagctggtttcagatgattttgttctgctgagcggcgatgatgcgagcgcgctggacttcatgca
attgggcggtcatggggttatttccgttacggctaacgtcgcagcgcgtgatatggcccagatgtgcaaactggcagca
gaagggcattttgccgaggcacgcgttattaatcagcgtctgatgccattacacaacaaactatttgtcgaacccaatc
caatcccggtgaaatgggcatgtaaggaactgggtcttgtggcgaccgatacgctgcgcctgccaatgacaccaatcac
cgacagtggtcgtgagacggtcagagcggcgcttaagcatgccggtttgctgtaataatactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagaacaatcagaacggttctgtctgcttgcttttaa
tgccataccaaacgtaccattgagacacttgtttgcacagaggatggtactagagaaagaggagaaatactagagaaag
aggagaaatactagatgtctattcaaaaaagtactaatagttcaagtttagcagaagtcattgaccgtattttagataa
aggaattgttattgatgcttttgcaagagtttctgttgtaggaattgaaattttaacgattgaagcgcgagtggttatt
gccagtgttgatacatggttacgctatgcagaagcagtagggcttcttcgtgacgacgtagaagaaaacggtcttcctg
aacgttcaaattcaagtgaagggcagccgcgttttagtatttaatatgaagtggtatggaaccttccttttttgagggt
ggttccagcttttagatctaactattggaggctactaaaaatggaaattaaaaaaattatgcaagccgtgaacgacttt
ttcggtgaacacgtagctcctcctcataaaattacctcggtggaagctactgaagatgaaggttggagagttattgttg
aagtcattgaagaacgagaatatatgaaaaaatacgccaaagatgaaatgctcggaacgtacgagtgctttgtaaataa
agaaaaagaagtcatttcattcaaacgactcgacgtcagatatagaagcgccattggcattgaagcataaacgataaga
tggcaggaggaacgtaaaaatgaccgtcttaacagacaaaaggaaaaaaggcagtggagcttttatacaagatgacgag
acaaaagaggttctttcaagagcgctgagctatttaaaatccggctattccattcattttacaggtcctgccggcggag
gcaaaacctctttagcgcgagcgcttgctaaaaagagaaagcgtcctgtaatgctgatgcacgggaatcacgagctcaa
caacaaagatttaattggcgattttacgggatacacgagcaaaaaagtaatcgaccagtacgttcgttctgtctataaa
aaagatgaacaggtgagtgaaaactggcaggatggccgattgcttgaagctgtaaaaaatggctatacgctgatttacg
acgaatttactcgttctaagcctgcgacgaataatatctttctatcgatattagaagaaggcgtgctgccgctgtatgg
agtaaaaatgaccgatccttttgtgcgcgtgcatcccgatttccgcgtcatcttcacaagcaatccagctgagtatgcc
ggcgtatatgatacgcaagatgcgcttctcgacaggttaattaccatgtttattgattataaagacatcgacagagaga
cagcgattttaacggagaaaacggacgtagaagaagatgaagcgcgcacaattgtaacgctcgtagcaaacgtgcgaaa
ccgctctggagacgaaaacagcagcggacttagcctgcgggcttcgcttatgatcgctacccttgccacgcagcaagac
attcctatcgatggaagtgacgaagattttcaaacgttatgtatcgatattttgcatcatccgcttaccaaatgtttgg
atgaagaaaatgcaaaaagcaaagccgaaaaaatcattttagaagaatgtaagaatatagacactgaagaaaagtaaag
gagcttgaaaacatgagtgaaacaaacgaaacaggtatttatatttttagcgccattcaaacggataaagacgaagaat
ttggcgccgtggaagtagaaggaacaaaagctgaaacatttttgattcgctacaaagacgcggctatggtagcagctga
agtaccgatgaaaatttatcatcctaatcgccaaaatttattaatgcatcaaaacgcagtagcagcgattatggacaag
aacgatacggttattccaatcagctttgggaatgtattcaaatcaaaagaagacgtaaaagttcttttggaaaaccttt
atccgcagtttgaaaagctgtttccagcgatcaaaggaaaaattgaagtcggtttaaaagtaattgggaaaaaagaatg
gcttgagaaaaaagtaaacgaaaatcctgaacttgagaaagtatcagcatccgtaaaaggaaaatcagaagcagccggt
tattatgagcgtattcaacttggaggaatggctcaaaagatgtttacttccctgcaaaaagaagtcaagacagatgtgt
tttctccgcttgaagaagcagcggaagcagcaaaagcaaatgagccaacgggcgaaacgatgcttttaaacgcgtcttt
cttaattaaccgagaagatgaagcgaagtttgatgaaaaagtaaatgaagcgcatgaaaactggaaagacaaagccgat
tttcattacagcggtccttggcctgcttataattttgtgaacattcgcctaaaagtagaagagaaataacgtgcttcac
aaattagtaaccgcacccattaaccttgtagtgaaaatcggcgaaaaagtacaggaagaagctgataaacagctatatg
accttccgacgattcagcaaaagctcattcagcttcaaatgatgtttgagcttggtgaaattccagaagaagcgtttca
agaaaaagaagatgaattgttaatgaggtacgaaattgcgaaacgcagagaaattgaacaatgggaagagctaacacaa
aaaagaaatgaggaatcctagatgggagaattactgtatttatacggtttaattccaacaaaagaagcagcagccatag
agccgtttccatcttataaggggtttgacggagaacattcactgtacccaattgcgtttgatcaggtgacggctgtagt
ttctaagctggatgctgacacctattcagaaaaagtgattcaagaaaaaatggagcaggatatgagctggctgcaagaa
aaagcatttcatcatcacgaaacggtagccgctttgtacgaagaatttacgatcattccattaaaattttgcaccattt
ataaaggtgaagaaagtctgcaagcagctattgagattaacaaagaaaagatagaaaattcactgacgctgcttcaagg
aaatgaagagtggaatgtgaaaatttactgtgatgatacagagcttaaaaaaggaatcagcgaaacgaatgaaagcgtg
aaagcgaaaaaacaagaaattagtcacttatcaccaggaagacagttttttgaaaagaaaaaaatagatcagctgattg
aaaaagaattagagcttcacaaaaacaaagtgtgtgaagagatacatgacaagctaaaagaattatcgctttatgactc
tgttaaaaagaattggagcaaagacgtaactggcgcagctgaacagatggcgtggaacagcgtgtttcttctcccgtct
ctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtc
tctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgt
ctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccg
tctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctccc
gtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcc
cgtctctgcaaattactaagttcgtaaacgaaatagaagagcttcagcaaaggcttgaaaataaaggctggaagtttga
agtgacgggaccatggccgccctatcatttctcgagctttgcgtaaagtgaggaattaacattatgtctcttaaacaat
ccatggagaataaagatattgctcttattgatattttagatgtcattttagataaaggagtcgccattaaaggagactt
aatcatttccatagctggcgtcgatttagtgtatttggatttgcgggtgcttatttcttcggttgaaacgcttgtgcaa
gcaaaagaaggaaatcacaaaccaatcacttctgaacaatttgataaacaaaaggaggaattaatggatgcaaccggtc
agccaagcaaatggacgaatccacttggatcctgatcaagctgaacaaggcttagcgcagcttgtgatgacagttattg
agctattgaggcaaatcgttgaacgtcatgccatgaggcgggtggagggtggaacgttgacggacgaacaaattgaaaa
cttaggaattgcactaatgaacttagaagaaaaaatggacgagttgaaagaggtgttcggtctggatgcagaagattta
aatattgatcttggaccgctaggcagcctgctttaagcggtcagtaggaggaacagtatggcagtcgaacataatatgc
agtcaagtacgattgtagatgtgctcgaaaagattttggataaaggagtcgttatagcgggggacatcaccgtaggaat
tgcagatgtcgagctattaacgataaagatccgcttgattgtggcttcggttgataaggcaaaagaaatcggcatggac
tggtgggaaaatgatccgtatctcagttcaaaaggagccaataacaaagcgctcgaagaagaaaataaaatgctgcatg
agcggttaaaaacgcttgaagaaaaaatagaaacgaaacgttaaaaactgtacgctacttaaaaaatggagggatttac
aatggcaactgaaacaaaattagataacacacaggcagaaaacaaggaaaataaaaatgcggaaaacggttcaaaagaa
aagaacggttcaaaagcaagcaaaacaacaagcagcgggccaatcaaacgagcggtagcaggaggcatcatcggtgcaa
cgattggatatgtatcgactcctgaaaatcgaaaaagtctccttgaccgcattgatacagacgaattaaaaagcaaagc
atctgatttaggaacaaaggtaaaagaaaaatcaaaaagcagcgtggccagcctgaaaacatctgcgggaagcttgttt
aaaaaagataaagataaatcaaaagatgatgaagaaaacgtaaattcttctagtagcgaaacagaagacgataacgttc
aagagtacgacgagttaaaagaagaaaatcaaactcttcaagatcgcttatcacagcttgaagaaaaaatgaacatgct
tgttgagcttagcctcaataaaaatcaagacgaagaagcggaagatacagattccgacgaagaagagaacgatgagaac
gatgaaaacgatgaaaacgagcaggacgatgaaaacgaagaagaaacatctaagccacgtaaaaaggataaaaaagaag
ctgaggaagaagaaagtgaaagtgacgaagacagcgaggaagaagaggaagattctcgctcaaacaaaaaaaataaaaa
agtaaaaacagaagaagaagacgaagatgaaagcgaagaagaaaaaaaggaagcgaaaccaaaaaagtcaacagctaaa
aaatcaaaaaatacaaaagcaaagaaaaacacggacgaagaagatgatgaagcaacatctctttctagtgaagacgata
caacagcctaagacgtaaaggaggaaagaaagacatgagtacaggcccttctttttcaactaaagacaatacgcttgaa
tactttgtgaaagcttctaataaacacggcttttcacttgatatttcattaaatgtaaacggcgctgtgatttccggta
ccatgatttcagcaaaagaatactttgattacttaagcgaaacgtttgaagaaggcagtgaagtggctcaggcgctaag
cgaacaattctctttagcaagcgaagcgagcgaatcaaacggagaagcagaagcccattttattcatttgaaaaataca
aagatttactgtggagacagtaaatctactccttctaaaggcaaaatcttttggagagggaaaatagcagaagtagacg
ggtttttcttaggaaagatttctgatgcaaaatcaacgagtaaaaagagttcataatactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttata