LOCUS BBa_K729008 3731 bp DNA linear 22-Nov-2024 DEFINITION pCstA+RBS+T7RNAP+pT7+RBS+GFP+TT ACCESSION BBa_K729008 VERSION BBa_K729008.1 FEATURES Location/Qualifiers misc_feature 1..42 /label=cAMP receptor protein binding site misc_feature 101..131 /label=RNA polymerase binding site promoter 1..131 /label=BBa_K118011 misc_feature 140..2817 /label=BBa_I712069 misc_feature 1..2817 /label=BBa_K729007 promoter 2826..2848 /label=BBa_I719005 misc_feature 2861..2864 /label=conserved RBS 2857..2868 /label=BBa_B0034 misc_feature 2875..2877 /label=start CDS 2875..3594 /label=superfolder GFP coding region misc_feature 3589..3594 /label=stop CDS 2875..3594 /label=BBa_I746916 stem_loop 3614..3657 /label=stem_loop misc_feature 3603..3682 /label=BBa_B0010 terminator 3603..3682 /label=BBa_B0010 stem_loop 3698..3717 /label=T7 TE misc_feature 3724..3724 /label=stop polyA_site 3718..3731 /label=polya misc_feature 3691..3731 /label=BBa_B0012 terminator 3691..3731 /label=BBa_B0012 misc_feature 2826..3731 /label=BBa_I746909 ORIGIN 1 actcggttaa cggagtgatc gagttaacat tgttaagtta aatattggtt tcaactccga 61 tttacatggt tgctgtgttg ttaaattgta caaagatgtt atagaaacaa aatgtaacat 121 ctctatggac atactagagc caccatgaac acgattaaca tcgctaagaa cgacttctct 181 gacatcgaac tggctgctat cccgttcaac actctggctg accattacgg tgagcgttta 241 gctcgcgaac agttggccct tgagcatgag tcttacgaga tgggtgaagc acgcttccgc 301 aagatgtttg agcgtcaact taaagctggt gaggttgcgg ataacgctgc cgccaagcct 361 ctcatcacta ccctactccc taagatgatt gcacgcatca acgactggtt tgaggaagtg 421 aaagctaagc gcggcaagcg cccgacagcc ttccagttcc tgcaagaaat caagccggaa 481 gccgtagcgt acatcaccat taagaccact ctggcttgcc taaccagtgc tgacaataca 541 accgttcagg ctgtagcaag cgcaatcggt cgggccattg aggacgaggc tcgcttcggt 601 cgtatccgtg accttgaagc taagcacttc aagaaaaacg ttgaggaaca actcaacaag 661 cgcgtagggc acgtctacaa gaaagcattt atgcaagttg tcgaggctga catgctctct 721 aagggtctac tcggtggcga ggcgtggtct tcgtggcata aggaagactc tattcatgta 781 ggagtacgct gcatcgagat gctcattgag tcaaccggaa tggttagctt acaccgccaa 841 aatgctggcg tagtaggtca agactctgag actatcgaac tcgcacctga atacgctgag 901 gctatcgcaa cccgtgcagg tgcgctggct ggcatctctc cgatgttcca accttgcgta 961 gttcctccta agccgtggac tggcattact ggtggtggct attgggctaa cggtcgtcgt 1021 cctctggcgc tggtgcgtac tcacagtaag aaagcactga tgcgctacga agacgtttac 1081 atgcctgagg tgtacaaagc gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag 1141 aaagtcctag cggtcgccaa cgtaatcacc aagtggaagc attgtccggt cgaggacatc 1201 cctgcgattg agcgtgaaga actcccgatg aaaccggaag acatcgacat gaatcctgag 1261 gctctcaccg cgtggaaacg tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag 1321 tctcgccgta tcagccttga gttcatgctt gagcaagcca ataagtttgc taaccataag 1381 gccatctggt tcccttacaa catggactgg cgcggtcgtg tttacgctgt gtcaatgttc 1441 aacccgcaag gtaacgatat gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc 1501 ggtaaggaag gttactactg gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag 1561 gttccgttcc ctgagcgcat caagttcatt gaggaaaacc acgagaacat catggcttgc 1621 gctaagtctc cactggagaa cacttggtgg gctgagcaag attctccgtt ctgcttcctt 1681 gcgttctgct ttgagtacgc tggggtacag caccacggcc tgagctataa ctgctccctt 1741 ccgctggcgt ttgacgggtc ttgctctggc atccagcact tctccgcgat gctccgagat 1801 gaggtaggtg gtcgcgcggt taacttgctt cctagtgaaa ccgttcagga catctacggg 1861 attgttgcta agaaagtcaa cgagattcta caagcagacg caatcaatgg gaccgataac 1921 gaagtagtta ccgtgaccga tgagaacact ggtgaaatct ctgagaaagt caagctgggc 1981 actaaggcac tggctggtca atggctggct tacggtgtta ctcgcagtgt gactaagcgt 2041 tcagtcatga cgctggctta cgggtccaaa gagttcggct tccgtcaaca agtgctggaa 2101 gataccattc agccagctat tgattccggc aagggtctga tgttcactca gccgaatcag 2161 gctgctggat acatggctaa gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg 2221 gttgaagcaa tgaactggct taagtctgct gctaagctgc tggctgctga ggtcaaagat 2281 aagaagactg gagagattct tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt 2341 ttccctgtgt ggcaggaata caagaagcct attcagacgc gcttgaacct gatgttcctc 2401 ggtcagttcc gcttacagcc taccattaac accaacaaag atagcgagat tgatgcacac 2461 aaacaggagt ctggtatcgc tcctaacttt gtacacagcc aagacggtag ccaccttcgt 2521 aagactgtag tgtgggcaca cgagaagtac ggaatcgaat cttttgcact gattcacgac 2581 tccttcggta ccattccggc tgacgctgcg aacctgttca aagcagtgcg cgaaactatg 2641 gttgacacat atgagtcttg tgatgtactg gctgatttct acgaccagtt cgctgaccag 2701 ttgcacgagt ctcaattgga caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc 2761 cgtgacatct tagagtcgga cttcgcgttc gcggatccaa aaaagaagag aaaggtatac 2821 tagagtaata cgactcacta tagggagata ctagagaaag aggagaaata ctagatgcgt 2881 aaaggcgaag agctgttcac tggtgtcgtc cctattctgg tggaactgga tggtgatgtc 2941 aacggtcata agttttccgt gcgtggcgag ggtgaaggtg acgcaactaa tggtaaactg 3001 acgctgaagt tcatctgtac tactggtaaa ctgccggtac cttggccgac tctggtaacg 3061 acgctgactt atggtgttca gtgctttgct cgttatccgg accatatgaa gcagcatgac 3121 ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac gcacgatttc ctttaaggat 3181 gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag gcgataccct ggtaaaccgc 3241 attgagctga aaggcattga ctttaaagaa gacggcaata tcctgggcca taagctggaa 3301 tacaatttta acagccacaa tgtttacatc accgccgata aacaaaaaaa tggcattaaa 3361 gcgaatttta aaattcgcca caacgtggag gatggcagcg tgcagctggc tgatcactac 3421 cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc cagacaatca ctatctgagc 3481 acgcaaagcg ttctgtctaa agatccgaac gagaaacgcg atcatatggt tctgctggag 3541 ttcgtaaccg cagcgggcat cacgcatggt atggatgaac tgtacaaatg atgatactag 3601 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 3661 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 3721 ctgcgtttat a //