BBa_K729008
type: ComponentDefinition
persistent identity: BBa K729008
display id: BBa_K729008
version: 1
was generated by: iGEM to SBOL conversion
description: pCstA+RBS+T7RNAP+pT7+RBS+GFP+TT
created: 2012-07-09T11:00:00Z
modified: 2015-05-08T01:13:06Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
experience: experience/Works
group u list: _975_
m user id: 0
owner id: 11516
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: -
star: false
creator: Erick Miguel Ramos-Martinez
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_I746909
component: BBa_K729007
sequence annotation: BBa_K729007
sequence annotation: BBa_I746909
sequence: https://synbiohub.org/public/igem/BBa_K729008_sequence/1

BBa_I746909
type: Component
persistent identity: component2193701
display id: component2193701
version: 1
top level: BBa_K729008
definition: BBa_I746909
access: public

BBa_K729007
type: Component
persistent identity: component2193687
display id: component2193687
version: 1
top level: BBa_K729008
definition: BBa_K729007
access: public

BBa_K729007
type: SequenceAnnotation
persistent identity: annotation2193687
display id: annotation2193687
version: 1
top level: BBa_K729008
location: https://synbiohub.org/public/igem/BBa_K729008/annotation2193687/range2193687/1
component: BBa_K729007

https://synbiohub.org/public/igem/BBa_K729008/annotation2193687/range2193687/1
type: Range
persistent identity: range2193687
display id: range2193687
version: 1
top level: BBa_K729008
direction: direction/reverse
start: 1
end: 2817
orientation: inline

BBa_I746909
type: SequenceAnnotation
persistent identity: annotation2193701
display id: annotation2193701
version: 1
top level: BBa_K729008
location: https://synbiohub.org/public/igem/BBa_K729008/annotation2193701/range2193701/1
component: BBa_I746909

https://synbiohub.org/public/igem/BBa_K729008/annotation2193701/range2193701/1
type: Range
persistent identity: range2193701
display id: range2193701
version: 1
top level: BBa_K729008
direction: direction/reverse
start: 2826
end: 3731
orientation: inline

BBa_I746909
type: ComponentDefinition
persistent identity: BBa I746909
display id: BBa_I746909
version: 1
was generated by: iGEM to SBOL conversion
description: superfolder GFP driven by T7 promoter
created: 2008-09-29T11:00:00Z
modified: 2015-08-31T04:08:05Z
mutable provenance: This part was created by standard assembly from construction intermediates: I746911 was put together with I746916:B0015
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is one of the constructs used to characterise superfolder GFP (see I746916 part description for source and other information about this GFP variant)
discontinued: false
dominant: false
experience: experience/Works
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no special considerations
star: true
creator: Stefan Milde
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0010
component: BBa_I746916
component: BBa_B0012
component: BBa_B0034
component: BBa_I719005
sequence annotation: BBa_I719005
sequence annotation: BBa_B0034
sequence annotation: BBa_I746916
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I746909_sequence/1

BBa_B0010
type: Component
persistent identity: component1977600
display id: component1977600
version: 1
top level: BBa_I746909
definition: BBa_B0010
access: public

BBa_I746916
type: Component
persistent identity: component1977599
display id: component1977599
version: 1
top level: BBa_I746909
definition: BBa_I746916
access: public

BBa_B0012
type: Component
persistent identity: component1977602
display id: component1977602
version: 1
top level: BBa_I746909
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component1977595
display id: component1977595
version: 1
top level: BBa_I746909
definition: BBa_B0034
access: public

BBa_I719005
type: Component
persistent identity: component1977593
display id: component1977593
version: 1
top level: BBa_I746909
definition: pT7
access: public

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation1977593
display id: annotation1977593
version: 1
top level: BBa_I746909
location: https://synbiohub.org/public/igem/BBa_I746909/annotation1977593/range1977593/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_I746909/annotation1977593/range1977593/1
type: Range
persistent identity: range1977593
display id: range1977593
version: 1
top level: BBa_I746909
direction: direction/reverse
start: 1
end: 23
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1977595
display id: annotation1977595
version: 1
top level: BBa_I746909
location: https://synbiohub.org/public/igem/BBa_I746909/annotation1977595/range1977595/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I746909/annotation1977595/range1977595/1
type: Range
persistent identity: range1977595
display id: range1977595
version: 1
top level: BBa_I746909
direction: direction/reverse
start: 32
end: 43
orientation: inline

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation1977599
display id: annotation1977599
version: 1
top level: BBa_I746909
location: https://synbiohub.org/public/igem/BBa_I746909/annotation1977599/range1977599/1
component: BBa_I746916

https://synbiohub.org/public/igem/BBa_I746909/annotation1977599/range1977599/1
type: Range
persistent identity: range1977599
display id: range1977599
version: 1
top level: BBa_I746909
direction: direction/reverse
start: 50
end: 769
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1977600
display id: annotation1977600
version: 1
top level: BBa_I746909
location: https://synbiohub.org/public/igem/BBa_I746909/annotation1977600/range1977600/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746909/annotation1977600/range1977600/1
type: Range
persistent identity: range1977600
display id: range1977600
version: 1
top level: BBa_I746909
direction: direction/forward
start: 778
end: 857
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1977602
display id: annotation1977602
version: 1
top level: BBa_I746909
location: https://synbiohub.org/public/igem/BBa_I746909/annotation1977602/range1977602/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746909/annotation1977602/range1977602/1
type: Range
persistent identity: range1977602
display id: range1977602
version: 1
top level: BBa_I746909
direction: direction/forward
start: 866
end: 906
orientation: inline

BBa_K729007
type: ComponentDefinition
persistent identity: BBa K729007
display id: BBa_K729007
version: 1
was generated by: iGEM to SBOL conversion
description: pcstA+RBS+T7RNAP
created: 2012-07-09T11:00:00Z
modified: 2015-05-08T01:13:06Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: true
experience: experience/None
group u list: _975_
m user id: 0
owner id: 11516
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Erick Miguel Ramos-Martinez
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I712069
component: BBa_K118011
sequence annotation: BBa_K118011
sequence annotation: BBa_I712069
sequence: https://synbiohub.org/public/igem/BBa_K729007_sequence/1

BBa_I712069
type: Component
persistent identity: component2193655
display id: component2193655
version: 1
top level: BBa_K729007
definition: BBa_I712069
access: public

BBa_K118011
type: Component
persistent identity: component2193654
display id: component2193654
version: 1
top level: BBa_K729007
definition: BBa_K118011
access: public

BBa_K118011
type: SequenceAnnotation
persistent identity: annotation2193654
display id: annotation2193654
version: 1
top level: BBa_K729007
location: https://synbiohub.org/public/igem/BBa_K729007/annotation2193654/range2193654/1
component: BBa_K118011

https://synbiohub.org/public/igem/BBa_K729007/annotation2193654/range2193654/1
type: Range
persistent identity: range2193654
display id: range2193654
version: 1
top level: BBa_K729007
direction: direction/reverse
start: 1
end: 131
orientation: inline

BBa_I712069
type: SequenceAnnotation
persistent identity: annotation2193655
display id: annotation2193655
version: 1
top level: BBa_K729007
location: https://synbiohub.org/public/igem/BBa_K729007/annotation2193655/range2193655/1
component: BBa_I712069

https://synbiohub.org/public/igem/BBa_K729007/annotation2193655/range2193655/1
type: Range
persistent identity: range2193655
display id: range2193655
version: 1
top level: BBa_K729007
direction: direction/reverse
start: 140
end: 2817
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_I712069
type: ComponentDefinition
persistent identity: BBa I712069
display id: BBa_I712069
version: 1
was generated by: iGEM to SBOL conversion
description: RBS-T7 RNA polymerase with NLS
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:46Z
mutable provenance: Genomic DNA of BL21 (E.coli strain).
owned by: james
owned by: myers
mutable description: T7 RNA polymerase with RBS (ribosome-binding site) and NLS (nuclear localisation signal). NLS is used to target the protein to the cell nucleus.
discontinued: false
dominant: true
experience: experience/None
group u list: _130_
m user id: 0
owner id: 1857
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: DNA was isolated from BL21 and PCR was performed to attach RBS and NLS.
star: true
creator: Sasa Jereb
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence: https://synbiohub.org/public/igem/BBa_I712069_sequence/1

pT7
type: ComponentDefinition
persistent identity: BBa I719005
display id: BBa_I719005
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Promoter
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:07:53Z
mutable provenance: ---
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Just a T7 Promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _128_
m user id: 0
owner id: 2097
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None
star: true
creator: Imperial 2007
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_I719005_sequence/1

BBa_K118011
type: ComponentDefinition
persistent identity: BBa K118011
display id: BBa_K118011
version: 1
was generated by: iGEM to SBOL conversion
description: PcstA (glucose-repressible promoter)
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: ''Eschaerichia coli'' JM109 genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the promoter for the ''Eschaerichia coli'' JM109 ''cstA'' gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (''cstA'' encodes the carbon starvation protein.)
discontinued: false
dominant: true
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations
star: true
creator: Andrew Hall
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cAMP receptor protein binding site
sequence annotation: RNA polymerase binding site
sequence: https://synbiohub.org/public/igem/BBa_K118011_sequence/1

cAMP receptor protein binding site
type: SequenceAnnotation
persistent identity: annotation1972435
display id: annotation1972435
version: 1
top level: BBa_K118011
location: https://synbiohub.org/public/igem/BBa_K118011/annotation1972435/range1972435/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K118011/annotation1972435/range1972435/1
type: Range
persistent identity: range1972435
display id: range1972435
version: 1
top level: BBa_K118011
direction: direction/forward
start: 1
end: 42
orientation: inline

RNA polymerase binding site
type: SequenceAnnotation
persistent identity: annotation1972436
display id: annotation1972436
version: 1
top level: BBa_K118011
location: https://synbiohub.org/public/igem/BBa_K118011/annotation1972436/range1972436/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K118011/annotation1972436/range1972436/1
type: Range
persistent identity: range1972436
display id: range1972436
version: 1
top level: BBa_K118011
direction: direction/forward
start: 101
end: 131
orientation: inline

BBa_I746916
type: ComponentDefinition
persistent identity: BBa I746916
display id: BBa_I746916
version: 1
was generated by: iGEM to SBOL conversion
description: superfolder GFP coding sequence
created: 2008-09-29T11:00:00Z
modified: 2015-08-31T04:08:05Z
mutable provenance: Superfolder GFP was originally described by: Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006 This version was synthesised de novo (by Geneart).
owned by: james
owned by: myers
mutable description: This is the coding sequence of superfolder GFP (Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006). It carries the following amino acid changes with respect to mut3 GFP (E0040), the currently most commonly used GFP in the registry: S30R, Y39N, F64L, G65T, F99S, N105T, Y145F, M153T, V163A, I171V, A206V Its in-vivo properties are considerably improved with respect to mut3 - it develops fluorescence about 3fold faster than mut3 GFP and reaches 4fold higher absolute fluorescence levels. Fluorescenct colonies can be identified with the naked eye even without UV or blue light illumination (that is to say the amount of blue light in normal daylight or lablight is sufficient). Additionally it is more stable in vitro and refolds faster after in vitro denaturation with respect to mut3 GFP. Note: Superfolder GFP is available in constructs driven by the pBAD and T7 promoters: part numbers I746908 and I746909 respectively. Additionally 6-his tagged versions for protein purification exist: I746914 (pBAD driven) and I746915 (T7 driven).
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Codon optimisation before de novo synthesis was carried out for both, E.coli and Bacillus subtilis.
star: false
creator: Stefan Milde
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: superfolder GFP coding region
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_I746916_sequence/1

superfolder GFP coding region
type: SequenceAnnotation
persistent identity: annotation1977534
display id: annotation1977534
version: 1
top level: BBa_I746916
location: https://synbiohub.org/public/igem/BBa_I746916/annotation1977534/range1977534/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I746916/annotation1977534/range1977534/1
type: Range
persistent identity: range1977534
display id: range1977534
version: 1
top level: BBa_I746916
direction: direction/forward
start: 1
end: 720
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1977533
display id: annotation1977533
version: 1
top level: BBa_I746916
location: https://synbiohub.org/public/igem/BBa_I746916/annotation1977533/range1977533/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I746916/annotation1977533/range1977533/1
type: Range
persistent identity: range1977533
display id: range1977533
version: 1
top level: BBa_I746916
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1977535
display id: annotation1977535
version: 1
top level: BBa_I746916
location: https://synbiohub.org/public/igem/BBa_I746916/annotation1977535/range1977535/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I746916/annotation1977535/range1977535/1
type: Range
persistent identity: range1977535
display id: range1977535
version: 1
top level: BBa_I746916
direction: direction/forward
start: 715
end: 720
orientation: inline

https://synbiohub.org/public/igem/BBa_K729008_sequence/1
type: Sequence
persistent identity: BBa K729008 sequence
display id: BBa_K729008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actcggttaacggagtgatcgagttaacattgttaagttaaatattggtttcaactccgatttacatggttgctgtgttgttaaattgtacaaagatgttatagaaacaaaatgtaacatctctatggacatactagagccaccatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746909_sequence/1
type: Sequence
persistent identity: BBa I746909 sequence
display id: BBa_I746909_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagaaagaggagaaatactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K729007_sequence/1
type: Sequence
persistent identity: BBa K729007 sequence
display id: BBa_K729007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actcggttaacggagtgatcgagttaacattgttaagttaaatattggtttcaactccgatttacatggttgctgtgttgttaaattgtacaaagatgttatagaaacaaaatgtaacatctctatggacatactagagccaccatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I712069_sequence/1
type: Sequence
persistent identity: BBa I712069 sequence
display id: BBa_I712069_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaccatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I719005_sequence/1
type: Sequence
persistent identity: BBa I719005 sequence
display id: BBa_I719005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118011_sequence/1
type: Sequence
persistent identity: BBa K118011 sequence
display id: BBa_K118011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actcggttaacggagtgatcgagttaacattgttaagttaaatattggtttcaactccgatttacatggttgctgtgttgttaaattgtacaaagatgttatagaaacaaaatgtaacatctctatggaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746916_sequence/1
type: Sequence
persistent identity: BBa I746916 sequence
display id: BBa_I746916_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z