BBa_K118011
1
BBa_K118011
PcstA (glucose-repressible promoter)
2008-08-18T11:00:00Z
2015-05-08T01:09:36Z
''Eschaerichia coli'' JM109 genomic DNA
Released HQ 2013
This is the promoter for the ''Eschaerichia coli'' JM109 ''cstA'' gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (''cstA'' encodes the carbon starvation protein.)
false
true
_192_
0
3282
9
In stock
true
No special considerations
true
Andrew Hall
annotation1972435
1
cAMP receptor protein binding site
range1972435
1
1
42
annotation1972436
1
RNA polymerase binding site
range1972436
1
101
131
BBa_K729015
1
BBa_K729015
pcstA+RBS+T7RNAP
2012-09-12T11:00:00Z
2015-05-08T01:13:06Z
E.coli
Released HQ 2013
This divice contains a glucose-repressible promoter ([http://partsregistry.org/Part:BBa_K118011 Part:BBa_K118011]) ligated with a GFP generator ([http://partsregistry.org/Part:BBa_E0840 Part:BBa_E0840]). Low glucose concentration promotes the transcription of the GFP as the complex between cAMP and CRP binds the cstA promoter.
true
false
_975_
0
13561
9
Discontinued
false
-
false
Erick Miguel Ramos-Martinez
annotation2185226
1
RNA polymerase binding site
range2185226
1
101
131
annotation2185227
1
BBa_K118011
range2185227
1
1
131
annotation2185225
1
cAMP receptor protein binding site
range2185225
1
1
42
BBa_K118011_sequence
1
actcggttaacggagtgatcgagttaacattgttaagttaaatattggtttcaactccgatttacatggttgctgtgttgttaaattgtacaaagatgttatagaaacaaaatgtaacatctctatggaca
BBa_K729015_sequence
1
actcggttaacggagtgatcgagttaacattgttaagttaaatattggtttcaactccgatttacatggttgctgtgttgttaaattgtacaaagatgttatagaaacaaaatgtaacatctctatggaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z