BBa_K730001 1 BBa_K730001 The mxaF gene is approximately 1.8 kb in size and encodes a 66-kDa polypeptide. 2012-09-25T11:00:00Z 2015-05-08T01:13:06Z GCAAGGTACCGCCGTTCCTCTTCCTGAATCGCCAGTGAGCGTTGGGCCAGCGCACGGTTT TCCTCGCGGGTTTTTTCCAGCGTGCCGACCGTGTGGTTGAAGGCCCTCGAAATGCGGGCG AATTCCGGCAGGTAGAACTCCGGCAGGCGGCGGCTGTAATCACCGTTTTCCAGGTCTTCC AGCCCTTCGAGGATGATGCCCACGGATTTGAACGCCCGGCCCAGGGTGGTGTGGACGACC AGGTACACCGTGACGGCGAGTGCCACCATCAGCAGCAGAAACCCCCGTGCTTCGATCCAG GCCTCGGCGATCTCGTCGGCCGGGTTGGCGTCAACGGTGATCTGGATCATCGGGTTGCCG ATCTGGTGCAGATGTTTCTCCGCCACCAGGGGGTCCGGCGCGACGGCCCAGGCGAACCAG GCGGGCGCTTCCTCACCCTCCGCGGGGCCGGCAGAGGAGGGCAGATCGATGATCGTCCTT TCGCCTTGATGGATGCTGAGGCTCAGGTGGCGGGCGTGGTCGAGCCGGGCGAGCTGGGCG ATCCATTCGACCGGCGAGCGACCCGCCGCCACCGCCTGTTCCAGGCCGGCATCGATCAGC TGTAACGCCAGGTTGACCGATGACCGGACTTCCTCGGCCACCGACCGGCGCGCGTTGTGG ACCACGAACAGGGTGCCCATGCCGATGATGGCCAGCATGACCAGACCGATCATGAGGTTC AGGCGAAACCGCAGACTCAAGGGCGCTTGCTCCCGGGAAAGATCGTATTAGTTTGCCTCG ATCGGCGGTCCTTGTGACAGGGAGATATTCCCGACGGATCCGGGGCATTCGAGCGGAACC GCCCGCCGTGGGAGTTTTTCCAGCGAGCATTCGAGAGTTTTTCAAGGCGGCTTCGAGGGG TTATTCCGTAACGCCGCCGACATGATCTGTCCCAGAATCTCCGCCGCTGTTCGTAGAGCG CCGATGCAGGGTCGGCATCAATCATTCTTGGAGGAGACAC Methylotrophic bacteria are a diverse group of microorganisms with the ability to utilize single-carbon (C1) substrates more reduced than carbon dioxide as their sole source of carbon and energy.Methanotrophs possesses native methanol-inducible promoters, notably promoters which are located upstream of genes that encode methanol dehydrogenase and other proteins required for its activity and enzymes required for the synthesis of the methanol dehydrogenase prosthetic group, pyrroloquinoline quinone. Of these, the promoter PmxaF has been thoroughly scrutinized both biochemically and in expression studies. In its native form in the chromosome, this strong promoter is methanol inducible. However, when this promoter is cloned in expression vectors, it acts essentially in a constitutive mode. The mxaF gene is approximately 1.8 kb in size and encodes a 66-kDa polypeptide. Our system involves the utilization of the methanol as an inducer for MxaF promoter. This would result in the diversion of the flux thus leading to a faster degradation of methane for the cell to survive. false false _976_ 0 11500 9 Not in stock false Yes selected 1kb upstream region false Antresh Kumar annotation2201220 1 mxaFpromoter range2201220 1 1 1000 BBa_K730001_sequence 1 gcaaggtaccgccgttcctcttcctgaatcgccagtgagcgttgggccagcgcacggttttcctcgcgggttttttccagcgtgccgaccgtgtggttgaaggccctcgaaatgcgggcgaattccggcaggtagaactccggcaggcggcggctgtaatcaccgttttccaggtcttccagcccttcgaggatgatgcccacggatttgaacgcccggcccagggtggtgtggacgaccaggtacaccgtgacggcgagtgccaccatcagcagcagaaacccccgtgcttcgatccaggcctcggcgatctcgtcggccgggttggcgtcaacggtgatctggatcatcgggttgccgatctggtgcagatgtttctccgccaccagggggtccggcgcgacggcccaggcgaaccaggcgggcgcttcctcaccctccgcggggccggcagaggagggcagatcgatgatcgtcctttcgccttgatggatgctgaggctcaggtggcgggcgtggtcgagccgggcgagctgggcgatccattcgaccggcgagcgacccgccgccaccgcctgttccaggccggcatcgatcagctgtaacgccaggttgaccgatgaccggacttcctcggccaccgaccggcgcgcgttgtggaccacgaacagggtgcccatgccgatgatggccagcatgaccagaccgatcatgaggttcaggcgaaaccgcagactcaagggcgcttgctcccgggaaagatcgtattagtttgcctcgatcggcggtccttgtgacagggagatattcccgacggatccggggcattcgagcggaaccgcccgccgtgggagtttttccagcgagcattcgagagtttttcaaggcggcttcgaggggttattccgtaacgccgccgacatgatctgtcccagaatctccgccgctgttcgtagagcgccgatgcagggtcggcatcaatcattcttggaggagacac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z