BBa_K731010
1
M256I CysE
M256I CysE (Serine Acetyltransferase)
2012-08-19T11:00:00Z
2015-05-08T01:13:06Z
PCR extraction from E. coli genome and mutagenesis.
Released HQ 2013
CysE is an enzyme involved In cysteine biosynthesis, also known as SAT. It catalyzes the acetylation of serine to give O-acetylserine, the CysE final precursor. Some O-acetylserine is also converted to N-acetylserine, which in turn triggers the assimilation of sulfate through specific genes.
This mutated version results to be not inhibited by Cys overproduction, as it secretes Cys.
false
false
_977_
0
12113
9
In stock
true
The construct has a strong RBS (AGGAGG) spaced 8nt from the start codon.
false
Jason Fontana, Daniele Rossetto, Giacomo Giacomelli
annotation2180205
1
Stop
range2180205
1
834
839
annotation2180201
1
RBS
range2180201
1
1
6
annotation2180204
1
Start
range2180204
1
15
17
annotation2180202
1
Spacer
range2180202
1
7
14
BBa_K731010_sequence
1
aggaggtttttattatgtcgtgtgaagaactggaaattgtctggaacaatattaaagccgaagccagaacgctggcggactgtgagccaatgctggccagtttttaccacgcgacgctactcaagcacgaaaaccttggcagtgcactgagctacatgctggcgaacaagctgtcatcgccaattatgcctgctattgctatccgtgaagtggtggaagaagcctacgccgctgacccggaaatgatcgcctctgcggcctgtgatattcaggcggtgcgtacccgcgacccggcagtcgataaatactcaaccccgttgttatacctgaagggttttcatgccttgcaggcctatcgcatcggtcactggttgtggaatcaggggcgtcgcgcactggcaatctttctgcaaaaccaggtttctgtgacgttccaggtcgatattcacccggcagcaaaaattggtcgcggtatcatgcttgaccacgcgacaggcatcgtcgttggtgaaacggcggtgattgaaaacgacgtatcgattctgcaatctgtgacgcttggcggtacgggtaaatctggtggtgaccgtcacccgaaaattcgtgaaggtgtgatgattggcgcgggcgcgaaaatcctcggcaatattgaagttgggcgcggcgcgaagattggcgcaggttccgtggtgctgcaaccggtgccgccgcataccaccgccgctggcgttccggctcgtattgtcggtaaaccagacagcgataagccatcaatggatatcgaccagcatttcaacggtattaaccatacatttgagtatggggatgggatctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z