BBa_K731010 1 M256I CysE M256I CysE (Serine Acetyltransferase) 2012-08-19T11:00:00Z 2015-05-08T01:13:06Z PCR extraction from E. coli genome and mutagenesis. Released HQ 2013 CysE is an enzyme involved In cysteine biosynthesis, also known as SAT. It catalyzes the acetylation of serine to give O-acetylserine, the CysE final precursor. Some O-acetylserine is also converted to N-acetylserine, which in turn triggers the assimilation of sulfate through specific genes. This mutated version results to be not inhibited by Cys overproduction, as it secretes Cys. false false _977_ 0 12113 9 In stock true The construct has a strong RBS (AGGAGG) spaced 8nt from the start codon. false Jason Fontana, Daniele Rossetto, Giacomo Giacomelli annotation2180205 1 Stop range2180205 1 834 839 annotation2180201 1 RBS range2180201 1 1 6 annotation2180204 1 Start range2180204 1 15 17 annotation2180202 1 Spacer range2180202 1 7 14 BBa_K731010_sequence 1 aggaggtttttattatgtcgtgtgaagaactggaaattgtctggaacaatattaaagccgaagccagaacgctggcggactgtgagccaatgctggccagtttttaccacgcgacgctactcaagcacgaaaaccttggcagtgcactgagctacatgctggcgaacaagctgtcatcgccaattatgcctgctattgctatccgtgaagtggtggaagaagcctacgccgctgacccggaaatgatcgcctctgcggcctgtgatattcaggcggtgcgtacccgcgacccggcagtcgataaatactcaaccccgttgttatacctgaagggttttcatgccttgcaggcctatcgcatcggtcactggttgtggaatcaggggcgtcgcgcactggcaatctttctgcaaaaccaggtttctgtgacgttccaggtcgatattcacccggcagcaaaaattggtcgcggtatcatgcttgaccacgcgacaggcatcgtcgttggtgaaacggcggtgattgaaaacgacgtatcgattctgcaatctgtgacgcttggcggtacgggtaaatctggtggtgaccgtcacccgaaaattcgtgaaggtgtgatgattggcgcgggcgcgaaaatcctcggcaatattgaagttgggcgcggcgcgaagattggcgcaggttccgtggtgctgcaaccggtgccgccgcataccaccgccgctggcgttccggctcgtattgtcggtaaaccagacagcgataagccatcaatggatatcgaccagcatttcaacggtattaaccatacatttgagtatggggatgggatctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z