LOCUS       BBa_K731040             2782 bp    DNA     linear       29-Nov-2024
DEFINITION  Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE
ACCESSION   BBa_K731040
VERSION     BBa_K731040.1  
FEATURES             Location/Qualifiers
     CDS             1..1080
                     /label=araC
     misc_feature    1..1203
                     /label=BBa_K731201
     promoter        1043..1114
                     /label=PC
     promoter        1080..1203
                     /label=pBAD
     RBS             1212..1217
                     /label=RBS
     misc_feature    1212..2044
                     /label=BBa_K731010
     misc_feature    1218..1225
                     /label=Spacer
     misc_feature    1226..1228
                     /label=Start
     misc_feature    2045..2062
                     /label=Linker
     misc_feature    1..2058
                     /label=BBa_K731030
     misc_feature    2063..2782
                     /label=sfGFP
     misc_feature    2776..2782
                     /label=Stop Codons
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac gaaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac tcgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtagtg ctcaaaagca gcttcgcctg
      181 actaatgcgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaacccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cgttaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccaccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgtactaga gaggaggttt ttattatgtc gtgtgaagaa ctggaaattg tctggaacaa
     1261 tattaaagcc gaagccagaa cgctggcgga ctgtgagcca atgctggcca gtttttacca
     1321 cgcgacgcta ctcaagcacg aaaaccttgg cagtgcactg agctacatgc tggcgaacaa
     1381 gctgtcatcg ccaattatgc ctgctattgc tatccgtgaa gtggtggaag aagcctacgc
     1441 cgctgacccg gaaatgatcg cctctgcggc ctgtgatatt caggcggtgc gtacccgcga
     1501 cccggcagtc gataaatact caaccccgtt gttatacctg aagggttttc atgccttgca
     1561 ggcctatcgc atcggtcact ggttgtggaa tcaggggcgt cgcgcactgg caatctttct
     1621 gcaaaaccag gtttctgtga cgttccaggt cgatattcac ccggcagcaa aaattggtcg
     1681 cggtatcatg cttgaccacg cgacaggcat cgtcgttggt gaaacggcgg tgattgaaaa
     1741 cgacgtatcg attctgcaat ctgtgacgct tggcggtacg ggtaaatctg gtggtgaccg
     1801 tcacccgaaa attcgtgaag gtgtgatgat tggcgcgggc gcgaaaatcc tcggcaatat
     1861 tgaagttggg cgcggcgcga agattggcgc aggttccgtg gtgctgcaac cggtgccgcc
     1921 gcataccacc gccgctggcg ttccggctcg tattgtcggt aaaccagaca gcgataagcc
     1981 atcaatggat atcgaccagc atttcaacgg tattaaccat acatttgagt atggggatgg
     2041 gatcggatcc ggcagcggtt ctatgcgtaa aggcgaagag ctgttcactg gtgtcgtccc
     2101 tattctggtg gaactggatg gtgatgtcaa cggtcataag ttttccgtgc gtggcgaggg
     2161 tgaaggtgac gcaactaatg gtaaactgac gctgaagttc atctgtacta ctggtaaact
     2221 gccggtacct tggccgactc tggtaacgac gctgacttat ggtgttcagt gctttgctcg
     2281 ttatccggac catatgaagc agcatgactt cttcaagtcc gccatgccgg aaggctatgt
     2341 gcaggaacgc acgatttcct ttaaggatga cggcacgtac aaaacgcgtg cggaagtgaa
     2401 atttgaaggc gataccctgg taaaccgcat tgagctgaaa ggcattgact ttaaagaaga
     2461 cggcaatatc ctgggccata agctggaata caattttaac agccacaatg tttacatcac
     2521 cgccgataaa caaaaaaatg gcattaaagc gaattttaaa attcgccaca acgtggagga
     2581 tggcagcgtg cagctggctg atcactacca gcaaaacact ccaatcggtg atggtcctgt
     2641 tctgctgcca gacaatcact atctgagcac gcaaagcgtt ctgtctaaag atccgaacga
     2701 gaaacgcgat catatggttc tgctggagtt cgtaaccgca gcgggcatca cgcatggtat
     2761 ggatgaactg tacaaataat aa
//