LOCUS       BBa_K731250             2087 bp    DNA     linear       25-Nov-2024
DEFINITION  Inducible araC-pBAD promoter with GFPmut3b, strong RBS
ACCESSION   BBa_K731250
VERSION     BBa_K731250.1  
FEATURES             Location/Qualifiers
     promoter        1080..1203
                     /label=pBAD
     promoter        1043..1114
                     /label=PC
     CDS             1..1080
                     /label=araC
     promoter        1..1203
                     /label=BBa_K731201
     RBS             1212..1224
                     /label=RBS-3\Weak
     RBS             1218..1221
                     /label=RBS
     misc_feature    1212..1224
                     /label=BBa_B0032
     RBS             1212..1224
                     /label=BBa_B0032
     CDS             1231..1950
                     /label=GFP protein
     CDS             1231..1950
                     /label=BBa_E0040
     stem_loop       1970..2013
                     /label=stem_loop
     misc_feature    1959..2038
                     /label=BBa_B0010
     terminator      1959..2038
                     /label=BBa_B0010
     stem_loop       2054..2073
                     /label=T7 TE
     misc_feature    2080..2080
                     /label=stop
     polyA_site      2074..2087
                     /label=polya
     misc_feature    2047..2087
                     /label=BBa_B0012
     terminator      2047..2087
                     /label=BBa_B0012
     misc_feature    1212..2087
                     /label=BBa_E0240
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac gaaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac tcgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtagtg ctcaaaagca gcttcgcctg
      181 actaatgcgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaacccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cgttaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccaccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgtactaga gtcacacagg aaagtactag atgcgtaaag gagaagaact tttcactgga
     1261 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
     1321 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
     1381 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
     1441 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
     1501 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
     1561 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
     1621 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
     1681 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
     1741 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
     1801 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
     1861 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
     1921 catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa
     1981 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     2041 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//