BBa_K731300 1 BBa_K731300 LacI + LacIq promoter (reverse) 2012-08-20T11:00:00Z 2015-05-08T01:13:06Z This part comes from the pET-21b plasmid This transcriptional regulator includes the LacI repressor and its constitutive promoter LacIq which operates in the reverse direction. The LacI repressor protein is inhibited by IPTG, and it is a very useful device to control and regulate the expression of a gene of interest. false false _977_ 0 11988 9 It's complicated true The start codon for LacI is GTG false Andrea Tassinari, Daniele Rossetto, Giacomo Giacomelli annotation2180533 1 stop range2180533 1 1 3 annotation2180532 1 LacI range2180532 1 1 1084 annotation2180534 1 start range2180534 1 1081 1084 annotation2180536 1 LacIq promoter range2180536 1 1093 1153 annotation2180535 1 rbs range2180535 1 1085 1092 BBa_K731300_sequence 1 tcactgcccgctttccagtcgggaaacctgtcgtgccagcctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z