LOCUS       BBa_K731400             2466 bp    DNA     linear       22-Nov-2024
DEFINITION  IPTG inducible Cysteine desulfhydrase (CysDes)
ACCESSION   BBa_K731400
VERSION     BBa_K731400.1  
FEATURES             Location/Qualifiers
     CDS             1..1084
                     /label=LacI
     misc_feature    1..3
                     /label=stop
     misc_feature    1081..1084
                     /label=start
     RBS             1085..1092
                     /label=rbs
     promoter        1093..1153
                     /label=LacIq promoter
     promoter        1..1153
                     /label=BBa_K731300
     promoter        1162..1191
                     /label=Ptac promoter
     misc_feature    1196..1220
                     /label=LacO operator
     RBS             1250..1255
                     /label=rbs
     CDS             1264..2466
                     /label=CysDes
     misc_feature    1264..1266
                     /label=start
     misc_feature    2461..2466
                     /label=stop
     CDS             1162..2466
                     /label=BBa_K731600
ORIGIN
        1 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ctgcattaat gaatcggcca
       61 acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg
      121 agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt
      181 ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat
      241 aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca
      301 gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca
      361 tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg
      421 cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat
      481 gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga
      541 tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg
      601 agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat
      661 tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca
      721 gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc
      781 ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg
      841 ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca
      901 acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca
      961 tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc
     1021 gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt
     1081 tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg
     1141 ttttgcgcca ttctactaga ggttgacaat taatcatcgg ctcgtataat gtgtgggaat
     1201 tgtgagcgga taacaattcc ctcatgaaat aattttgttt aactttaaga ggaggttttt
     1261 attatgatct atgactttac caccaaaatc tcgcgcaaaa atctgggcag cctgaaatgg
     1321 gacctgatgt actcgcagaa cccggaagtg ggcaacgaag tggttccgct gagcgtggcc
     1381 gatatggaat ttaaaaatcc gccggaactg attgaaggtc tgaaaaaata tctggacgaa
     1441 acggtgctgg gctatacggg tccgaccgaa gaatacaaga aaaccgttaa aaaatggatg
     1501 aaagatcgtc atcagtggga tattgaaacg gactggatta tcaacaccgc tggcgtcgtg
     1561 ccggcagtgt ttaatgctgt tcgcgaattt acgaaaccgg gcgatggtgt cattatcatt
     1621 accccggtgt attacccgtt tttcatggcg atcaaaaacc aagaacgtaa aatcatcgaa
     1681 tgcgaactgc tggaaaaaga tggctattac accatcgact ttgaaaaact ggaaaaactg
     1741 agtaaagata aaaacaacaa agccctgctg ttttgttcac cgcacaatcc ggttggtcgc
     1801 gtctggaaaa aagatgaact gcaaaaaatc aaagatatcg tcctgaaatc agacctgatg
     1861 ctgtggtcgg atgaaattca ttttgacctg atcatgccgg gttatgaaca cacggttttc
     1921 cagtccattg atgaacaact ggcagacaaa accatcacgt ttaccgctcc gagcaaaacc
     1981 ttcaacattg cgggcatggg catgtctaac atcatcatca aaaacccgga tatccgtgaa
     2041 cgctttacga aaagtcgtga catcacctcc ggcatgccgt tcaccacgct gggttacaaa
     2101 gcatgcgaaa tttgttacaa agaatgcggc aaatggctgg atggttgtat taaagtgatc
     2161 gacaaaaacc agcgtatcgt taaagatttc tttgaagtca accatccgga aattaaagcc
     2221 ccgctgatcg aaggcaccta tctgcaatgg attgattttc gcgcgctgaa aatggaccat
     2281 aaagccatgg aagaatttat gatccacaaa gcacaaatct ttttcgatga aggctacatt
     2341 tttggtgacg gcggtattgg tttcgaacgc atcaatctgg cggccccgag ctctgttatc
     2401 caagaatcac tggaacgcct gaacaaagca ctgaaagacc tgaaaaaccg tcacctgaaa
     2461 taataa
//