BBa_K731400 1 BBa_K731400 IPTG inducible Cysteine desulfhydrase (CysDes) 2012-08-20T11:00:00Z 2015-05-08T01:13:06Z LacI+LacIq promoter comes from pET-21b plasmid. CysDes gene comes from the genome of Treponema denticola. This composite part was designed to construct a working CysDes gene under the control of an inducible promoter: the presence of IPTG inhibits the LacI repressor protein binding to the LacO operator; in this way it allows the RNApol to bind to the Ptac promoter and trascribe the gene of interest. In this case the gene of interest is a Cysteine Desulfhydrase, an aminotransferase that converts cysteine into pyruvate, ammonia and hydrogen sulfide. false false _977_ 0 11988 9 It's complicated true LacI+LacIq promoter is an autonomous part (K731300) also designed by our team. false Andrea Tassinari component2180994 1 BBa_K731300 component2181001 1 BBa_K731600 annotation2181001 1 BBa_K731600 range2181001 1 1162 2466 annotation2180994 1 BBa_K731300 range2180994 1 1 1153 BBa_K731600 1 BBa_K731600 Cysteine Desulfhydrase (CysDes) 2012-08-22T11:00:00Z 2015-05-08T01:13:06Z CysDes nucleotide sequence was synthesized by Genscript and subcloned into psB1C3 to create part BBa_K731600. This part encode for a cysteine desulfhydrase from Treponema denticola, which is known to be a strong enzyme that produces H2S, ammonia and pyruvate starting from cysteine. It is often indicated also as Hly. false false _977_ 0 11988 9 Not in stock false The nucleotide sequence of CysDes was optimized by Genscript, thus it does not correspond to the natural sequence found in Treponema denticola. The sequence of the lac operator corresponds to the sequence found in pET21b. false Andrea Tassinari, Daniele Rossetto, Giacomo Giacomelli annotation2180940 1 Ptac promoter range2180940 1 1 30 annotation2180944 1 start range2180944 1 103 105 annotation2180942 1 rbs range2180942 1 89 94 annotation2180941 1 LacO operator range2180941 1 35 59 annotation2180943 1 CysDes range2180943 1 103 1305 annotation2180945 1 stop range2180945 1 1300 1305 BBa_K731300 1 BBa_K731300 LacI + LacIq promoter (reverse) 2012-08-20T11:00:00Z 2015-05-08T01:13:06Z This part comes from the pET-21b plasmid This transcriptional regulator includes the LacI repressor and its constitutive promoter LacIq which operates in the reverse direction. The LacI repressor protein is inhibited by IPTG, and it is a very useful device to control and regulate the expression of a gene of interest. false false _977_ 0 11988 9 It's complicated true The start codon for LacI is GTG false Andrea Tassinari, Daniele Rossetto, Giacomo Giacomelli annotation2180534 1 start range2180534 1 1081 1084 annotation2180532 1 LacI range2180532 1 1 1084 annotation2180536 1 LacIq promoter range2180536 1 1093 1153 annotation2180533 1 stop range2180533 1 1 3 annotation2180535 1 rbs range2180535 1 1085 1092 BBa_K731600_sequence 1 gttgacaattaatcatcggctcgtataatgtgtgggaattgtgagcggataacaattccctcatgaaataattttgtttaactttaagaggaggtttttattatgatctatgactttaccaccaaaatctcgcgcaaaaatctgggcagcctgaaatgggacctgatgtactcgcagaacccggaagtgggcaacgaagtggttccgctgagcgtggccgatatggaatttaaaaatccgccggaactgattgaaggtctgaaaaaatatctggacgaaacggtgctgggctatacgggtccgaccgaagaatacaagaaaaccgttaaaaaatggatgaaagatcgtcatcagtgggatattgaaacggactggattatcaacaccgctggcgtcgtgccggcagtgtttaatgctgttcgcgaatttacgaaaccgggcgatggtgtcattatcattaccccggtgtattacccgtttttcatggcgatcaaaaaccaagaacgtaaaatcatcgaatgcgaactgctggaaaaagatggctattacaccatcgactttgaaaaactggaaaaactgagtaaagataaaaacaacaaagccctgctgttttgttcaccgcacaatccggttggtcgcgtctggaaaaaagatgaactgcaaaaaatcaaagatatcgtcctgaaatcagacctgatgctgtggtcggatgaaattcattttgacctgatcatgccgggttatgaacacacggttttccagtccattgatgaacaactggcagacaaaaccatcacgtttaccgctccgagcaaaaccttcaacattgcgggcatgggcatgtctaacatcatcatcaaaaacccggatatccgtgaacgctttacgaaaagtcgtgacatcacctccggcatgccgttcaccacgctgggttacaaagcatgcgaaatttgttacaaagaatgcggcaaatggctggatggttgtattaaagtgatcgacaaaaaccagcgtatcgttaaagatttctttgaagtcaaccatccggaaattaaagccccgctgatcgaaggcacctatctgcaatggattgattttcgcgcgctgaaaatggaccataaagccatggaagaatttatgatccacaaagcacaaatctttttcgatgaaggctacatttttggtgacggcggtattggtttcgaacgcatcaatctggcggccccgagctctgttatccaagaatcactggaacgcctgaacaaagcactgaaagacctgaaaaaccgtcacctgaaataataa BBa_K731400_sequence 1 tcactgcccgctttccagtcgggaaacctgtcgtgccagcctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattctactagaggttgacaattaatcatcggctcgtataatgtgtgggaattgtgagcggataacaattccctcatgaaataattttgtttaactttaagaggaggtttttattatgatctatgactttaccaccaaaatctcgcgcaaaaatctgggcagcctgaaatgggacctgatgtactcgcagaacccggaagtgggcaacgaagtggttccgctgagcgtggccgatatggaatttaaaaatccgccggaactgattgaaggtctgaaaaaatatctggacgaaacggtgctgggctatacgggtccgaccgaagaatacaagaaaaccgttaaaaaatggatgaaagatcgtcatcagtgggatattgaaacggactggattatcaacaccgctggcgtcgtgccggcagtgtttaatgctgttcgcgaatttacgaaaccgggcgatggtgtcattatcattaccccggtgtattacccgtttttcatggcgatcaaaaaccaagaacgtaaaatcatcgaatgcgaactgctggaaaaagatggctattacaccatcgactttgaaaaactggaaaaactgagtaaagataaaaacaacaaagccctgctgttttgttcaccgcacaatccggttggtcgcgtctggaaaaaagatgaactgcaaaaaatcaaagatatcgtcctgaaatcagacctgatgctgtggtcggatgaaattcattttgacctgatcatgccgggttatgaacacacggttttccagtccattgatgaacaactggcagacaaaaccatcacgtttaccgctccgagcaaaaccttcaacattgcgggcatgggcatgtctaacatcatcatcaaaaacccggatatccgtgaacgctttacgaaaagtcgtgacatcacctccggcatgccgttcaccacgctgggttacaaagcatgcgaaatttgttacaaagaatgcggcaaatggctggatggttgtattaaagtgatcgacaaaaaccagcgtatcgttaaagatttctttgaagtcaaccatccggaaattaaagccccgctgatcgaaggcacctatctgcaatggattgattttcgcgcgctgaaaatggaccataaagccatggaagaatttatgatccacaaagcacaaatctttttcgatgaaggctacatttttggtgacggcggtattggtttcgaacgcatcaatctggcggccccgagctctgttatccaagaatcactggaacgcctgaacaaagcactgaaagacctgaaaaaccgtcacctgaaataataa BBa_K731300_sequence 1 tcactgcccgctttccagtcgggaaacctgtcgtgccagcctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z