LOCUS       BBa_K731480             3194 bp    DNA     linear       26-Nov-2024
DEFINITION  IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)
ACCESSION   BBa_K731480
VERSION     BBa_K731480.1  
FEATURES             Location/Qualifiers
     CDS             1..1083
                     /label=LacI
     misc_feature    1..3
                     /label=stop
     misc_feature    1081..1083
                     /label=start
     promoter        1093..1152
                     /label=LacIq promoter
     promoter        1161..1190
                     /label=Ptac promoter
     misc_feature    1195..1219
                     /label=LacO operator
     RBS             1249..1255
                     /label=rbs
     CDS             1263..2459
                     /label=CysDes
     CDS             2478..3194
                     /label=superfolder GFP coding region
     misc_feature    2478..2480
                     /label=start
     misc_feature    3192..3194
                     /label=stop
ORIGIN
        1 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
       61 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
      121 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
      181 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
      241 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
      301 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
      361 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
      421 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
      481 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
      541 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
      601 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
      661 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
      721 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
      781 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
      841 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
      901 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
      961 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     1021 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     1081 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     1141 tttgcgccat tctactagag gttgacaatt aatcatcggc tcgtataatg tgtgggaatt
     1201 gtgagcggat aacaattccc tcatgaaata attttgttta actttaagag gaggttttta
     1261 ttatgatcta tgactttacc accaaaatct cgcgcaaaaa tctgggcagc ctgaaatggg
     1321 acctgatgta ctcgcagaac ccggaagtgg gcaacgaagt ggttccgctg agcgtggccg
     1381 atatggaatt taaaaatccg ccggaactga ttgaaggtct gaaaaaatat ctggacgaaa
     1441 cggtgctggg ctatacgggt ccgaccgaag aatacaagaa aaccgttaaa aaatggatga
     1501 aagatcgtca tcagtgggat attgaaacgg actggattat caacaccgct ggcgtcgtgc
     1561 cggcagtgtt taatgctgtt cgcgaattta cgaaaccggg cgatggtgtc attatcatta
     1621 ccccggtgta ttacccgttt ttcatggcga tcaaaaacca agaacgtaaa atcatcgaat
     1681 gcgaactgct ggaaaaagat ggctattaca ccatcgactt tgaaaaactg gaaaaactga
     1741 gtaaagataa aaacaacaaa gccctgctgt tttgttcacc gcacaatccg gttggtcgcg
     1801 tctggaaaaa agatgaactg caaaaaatca aagatatcgt cctgaaatca gacctgatgc
     1861 tgtggtcgga tgaaattcat tttgacctga tcatgccggg ttatgaacac acggttttcc
     1921 agtccattga tgaacaactg gcagacaaaa ccatcacgtt taccgctccg agcaaaacct
     1981 tcaacattgc gggcatgggc atgtctaaca tcatcatcaa aaacccggat atccgtgaac
     2041 gctttacgaa aagtcgtgac atcacctccg gcatgccgtt caccacgctg ggttacaaag
     2101 catgcgaaat ttgttacaaa gaatgcggca aatggctgga tggttgtatt aaagtgatcg
     2161 acaaaaacca gcgtatcgtt aaagatttct ttgaagtcaa ccatccggaa attaaagccc
     2221 cgctgatcga aggcacctat ctgcaatgga ttgattttcg cgcgctgaaa atggaccata
     2281 aagccatgga agaatttatg atccacaaag cacaaatctt tttcgatgaa ggctacattt
     2341 ttggtgacgg cggtattggt ttcgaacgca tcaatctggc ggccccgagc tctgttatcc
     2401 aagaatcact ggaacgcctg aacaaagcac tgaaagacct gaaaaaccgt cacctgaaag
     2461 gatccggcag cggttctatg cgtaaaggcg aagagctgtt cactggtgtc gtccctattc
     2521 tggtggaact ggatggtgat gtcaacggtc ataagttttc cgtgcgtggc gagggtgaag
     2581 gtgacgcaac taatggtaaa ctgacgctga agttcatctg tactactggt aaactgccgg
     2641 taccttggcc gactctggta acgacgctga cttatggtgt tcagtgcttt gctcgttatc
     2701 cggaccatat gaagcagcat gacttcttca agtccgccat gccggaaggc tatgtgcagg
     2761 aacgcacgat ttcctttaag gatgacggca cgtacaaaac gcgtgcggaa gtgaaatttg
     2821 aaggcgatac cctggtaaac cgcattgagc tgaaaggcat tgactttaaa gaagacggca
     2881 atatcctggg ccataagctg gaatacaatt ttaacagcca caatgtttac atcaccgccg
     2941 ataaacaaaa aaatggcatt aaagcgaatt ttaaaattcg ccacaacgtg gaggatggca
     3001 gcgtgcagct ggctgatcac taccagcaaa acactccaat cggtgatggt cctgttctgc
     3061 tgccagacaa tcactatctg agcacgcaaa gcgttctgtc taaagatccg aacgagaaac
     3121 gcgatcatat ggttctgctg gagttcgtaa ccgcagcggg catcacgcat ggtatggatg
     3181 aactgtacaa ataa
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