BBa_K731480 1 BBa_K731480 IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes) 2012-08-21T11:00:00Z 2015-05-08T01:13:06Z LacI+LacIq promoter comes from pET-21b plasmid. CysDes gene comes from the genome of Treponema denticola. This composite part is the same as BBa_K731400 but with the add of a sfGFP (superfolder GFP) downstream. This reporter gene is very useful to check and measure the expression of an upstream gene, that in this case codes for a cysteine desulfhydrase; its role is converting cysteine into pyruvate, ammonia and hydrogen sulfide. false false _977_ 0 11988 9 In stock true The sfGFP is the BioBrick BBa_K515005 designed by iGEM11_Imperial_College_London. false Andrea Tassinari, Francesco Guzzonato annotation2181531 1 LacIq promoter range2181531 1 1093 1152 annotation2181532 1 Ptac promoter range2181532 1 1161 1190 annotation2181534 1 rbs range2181534 1 1249 1255 annotation2181538 1 stop range2181538 1 3192 3194 annotation2181536 1 superfolder GFP coding region range2181536 1 2478 3194 annotation2181530 1 start range2181530 1 1081 1083 annotation2181533 1 LacO operator range2181533 1 1195 1219 annotation2181528 1 LacI range2181528 1 1 1083 annotation2181535 1 CysDes range2181535 1 1263 2459 annotation2181537 1 start range2181537 1 2478 2480 annotation2181529 1 stop range2181529 1 1 3 BBa_K731480_sequence 1 tcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattctactagaggttgacaattaatcatcggctcgtataatgtgtgggaattgtgagcggataacaattccctcatgaaataattttgtttaactttaagaggaggtttttattatgatctatgactttaccaccaaaatctcgcgcaaaaatctgggcagcctgaaatgggacctgatgtactcgcagaacccggaagtgggcaacgaagtggttccgctgagcgtggccgatatggaatttaaaaatccgccggaactgattgaaggtctgaaaaaatatctggacgaaacggtgctgggctatacgggtccgaccgaagaatacaagaaaaccgttaaaaaatggatgaaagatcgtcatcagtgggatattgaaacggactggattatcaacaccgctggcgtcgtgccggcagtgtttaatgctgttcgcgaatttacgaaaccgggcgatggtgtcattatcattaccccggtgtattacccgtttttcatggcgatcaaaaaccaagaacgtaaaatcatcgaatgcgaactgctggaaaaagatggctattacaccatcgactttgaaaaactggaaaaactgagtaaagataaaaacaacaaagccctgctgttttgttcaccgcacaatccggttggtcgcgtctggaaaaaagatgaactgcaaaaaatcaaagatatcgtcctgaaatcagacctgatgctgtggtcggatgaaattcattttgacctgatcatgccgggttatgaacacacggttttccagtccattgatgaacaactggcagacaaaaccatcacgtttaccgctccgagcaaaaccttcaacattgcgggcatgggcatgtctaacatcatcatcaaaaacccggatatccgtgaacgctttacgaaaagtcgtgacatcacctccggcatgccgttcaccacgctgggttacaaagcatgcgaaatttgttacaaagaatgcggcaaatggctggatggttgtattaaagtgatcgacaaaaaccagcgtatcgttaaagatttctttgaagtcaaccatccggaaattaaagccccgctgatcgaaggcacctatctgcaatggattgattttcgcgcgctgaaaatggaccataaagccatggaagaatttatgatccacaaagcacaaatctttttcgatgaaggctacatttttggtgacggcggtattggtttcgaacgcatcaatctggcggccccgagctctgttatccaagaatcactggaacgcctgaacaaagcactgaaagacctgaaaaaccgtcacctgaaaggatccggcagcggttctatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z