LOCUS       BBa_K731520             2123 bp    DNA     linear       04-May-2024
DEFINITION  IPTG inducible GFPmut3b
ACCESSION   BBa_K731520
VERSION     BBa_K731520.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=stop
     CDS             1..1084
                     /label=LacI
     misc_feature    1081..1084
                     /label=start
     RBS             1085..1092
                     /label=rbs
     promoter        1093..1153
                     /label=LacIq promoter
     misc_feature    1..1153
                     /label=BBa_K731300
     misc_feature    1194..1219
                     /label=lacO operator
     promoter        1162..1188
                     /label=Ptac promoter
     promoter        1..1239
                     /label=BBa_K731500
     RBS             1248..1260
                     /label=RBS-3\Weak
     RBS             1254..1257
                     /label=RBS
     misc_feature    1248..1260
                     /label=BBa_B0032
     RBS             1248..1260
                     /label=BBa_B0032
     CDS             1267..1986
                     /label=GFP protein
     CDS             1267..1986
                     /label=BBa_E0040
     stem_loop       2006..2049
                     /label=stem_loop
     misc_feature    1995..2074
                     /label=BBa_B0010
     terminator      1995..2074
                     /label=BBa_B0010
     stem_loop       2090..2109
                     /label=T7 TE
     misc_feature    2116..2116
                     /label=stop
     polyA_site      2110..2123
                     /label=polya
     misc_feature    2083..2123
                     /label=BBa_B0012
     terminator      2083..2123
                     /label=BBa_B0012
     misc_feature    1248..2123
                     /label=BBa_E0240
ORIGIN
        1 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
       61 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
      121 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
      181 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
      241 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
      301 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
      361 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
      421 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
      481 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
      541 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
      601 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
      661 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
      721 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
      781 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
      841 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
      901 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
      961 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     1021 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     1081 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     1141 tttgcgccat tctactagag gttgacaatt aatcatcggc tcgtataatg tgtgggaatt
     1201 gtgagcggat aacaattccc tcatgaaata attttgtttt actagagtca cacaggaaag
     1261 tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
     1321 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
     1381 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
     1441 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg
     1501 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
     1561 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
     1621 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
     1681 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
     1741 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta
     1801 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
     1861 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
     1921 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
     1981 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     2041 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     2101 gggtgggcct ttctgcgttt ata
//