LOCUS BBa_K731711 6942 bp DNA linear 22-Nov-2024 DEFINITION BBa_K731710 backbone with T7terminator (BBa_K731721) ACCESSION BBa_K731711 VERSION BBa_K731711.1 FEATURES Location/Qualifiers CDS 36..752 /label=A206K VENUS misc_feature 843..890 /label=T7 TERMINATOR CDS 1711..2370 /label=ampicillin resistance gene CDS 4561..5653 /label=lacI promoter 6033..6061 /label=Ptac misc_feature 6065..6087 /label=lacO CDS 6134..6844 /label=mCHERRY misc_feature 1..6886 /label=BBa_K731710 misc_feature 6895..6942 /label=T7 terminator terminator 6895..6942 /label=BBa_K731721 ORIGIN 1 tactagtagc ggccgctgca gaaggagaat aatctatgag caaaggcgaa gaactgttca 61 cgggtgtggt tccgatcctg gttgaactgg atggcgatgt gaacggtcat aaatttagcg 121 tgtctggtga aggcgaaggt gatgcgacct acggcaaact gacgctgaaa ctgatttgca 181 ccacgggtaa actgccggtt ccgtggccga ccctggtgac cacgctgggt tatggtctga 241 tgtgtttcgc acgttacccg gatcacatga aacgccatga tttctttaaa tctgcgatgc 301 cggaaggcta tgtgcaggaa cgtaccatct ttttcaaaga tgatggtaac tacaaaaccc 361 gcgcggaagt taaatttgaa ggcgatacgc tggtgaaccg tattgaactg aaaggtatcg 421 atttcaaaga agatggcaat attctgggtc acaaactgga atacaactac aacagtcata 481 acgtgtacat taccgccgat aaacagaaaa acggtatcaa agcaaacttc aaaatccgtc 541 acaacatcga agatggcggt gttcagctgg ccgatcatta ccagcagaac accccgattg 601 gcgatggtcc ggtgctgctg ccggataatc attatctgag ttaccagagc aaactgtcta 661 aagatccgaa tgaaaaacgc gatcacatgg ttctgctgga atttgtgacc gcggccggca 721 ttacgcatgg tatggatgaa ctgtataaat aactcgagca ccaccaccac caccactgag 781 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat 841 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag 901 gaactatatc cggatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt 961 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 1021 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 1081 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 1141 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 1201 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 1261 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 1321 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt 1381 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 1441 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 1501 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 1561 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 1621 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 1681 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 1741 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 1801 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 1861 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 1921 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 1981 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 2041 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 2101 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 2161 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 2221 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 2281 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 2341 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 2401 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 2461 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 2521 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 2581 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 2641 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 2701 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 2761 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 2821 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 2881 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 2941 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 3001 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 3061 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 3121 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 3181 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 3241 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 3301 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca 3361 caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt 3421 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc 3481 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 3541 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca 3601 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc 3661 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc 3721 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct 3781 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga 3841 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg 3901 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg 3961 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg 4021 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt 4081 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga 4141 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag 4201 cacgatcatg cgcacccgtg gggccgccat gccggcgata atggcctgct tctcgccgaa 4261 acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac 4321 cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac 4381 ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc 4441 ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa 4501 gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg 4561 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 4621 acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg 4681 agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt 4741 ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat 4801 aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca 4861 gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca 4921 tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg 4981 cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat 5041 gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga 5101 tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg 5161 agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat 5221 tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca 5281 gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc 5341 ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg 5401 ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca 5461 acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca 5521 tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc 5581 gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt 5641 tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg 5701 ttttgcgcca ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt 5761 aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca 5821 tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg 5881 aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg 5941 atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg 6001 tagaggatcg agatctcgat cccgcgaaat gttgacaatt aatcatcggc tcgtataatg 6061 tgtgggaatt gtgagcggat aacaattccc ctcaagaaat aattttgttt aactttaaga 6121 aggagatata catatggtga gtaaaggcga ggaggacaat atggcgatca tcaaagagtt 6181 catgcgcttc aaagtccaca tggaaggcag cgttaatggt cacgagttcg aaattgaggg 6241 cgaaggcgaa ggtcgtccgt atgagggtac acagaccgct aaactgaaag tcacgaaagg 6301 tggtccactg ccatttgctt gggatattct gagcccacag ttcatgtatg gctccaaagc 6361 ctatgtgaaa catccggccg atattccgga ctatctgaaa ctgagcttcc ctgaagggtt 6421 caaatgggaa cgtgtgatga actttgagga tggtggtgtt gtgacagtga cacaggattc 6481 tagcctgcaa gacggtgagt tcatctataa agtgaaactg cgtggcacga attttccgag 6541 tgatggcccg gttatgcaga aaaaaacgat gggttgggag gcctctagtg agcgtatgta 6601 tccagaagat ggcgctctga aaggcgaaat caaacagcgt ctgaaactga aagatggtgg 6661 ccactatgat gccgaagtga aaaccacgta taaagccaaa aaacctgtcc aactgcctgg 6721 tgcctataac gttaacatca aactggacat cacctcacac aatgaggact atacgatcgt 6781 ggagcagtat gagcgtgctg aaggacgtca ttctaccggt ggtatggatg agctgtataa 6841 ataagcggat cctaattcaa ttaggaattc gcggccgctt ctagagtact agagctagca 6901 taaccccttg gggcctctaa acgggtcttg aggggttttt tg //