BBa_K731712 1 BBa_K731712 BBa_K731710 with BBa_K731722 E. coli terminator 2012-08-23T11:00:00Z 2015-05-08T01:13:06Z [[Part:BBa_K731710|BBa_K731710]] + [[Part:BBa_K731722|BBa_K731722]] Coli terminator Coli polymerase testing construct 2 false false _977_ 0 12030 9 It's complicated false BBa_K731722 presents 1 less base at the 3' of the sequence if alligned with BBa_B0010. This doesn't affect the termination efficiency. false Giacomo Giacomelli, Anna Depetris component2180771 1 BBa_K731710 component2180773 1 BBa_K731722 annotation2180771 1 BBa_K731710 range2180771 1 1 6886 annotation2180773 1 BBa_K731722 range2180773 1 6887 6965 BBa_K731722 1 BBa_K731722 T1 terminator from E. coli rrnB 2012-08-22T11:00:00Z 2015-05-08T01:13:06Z This part comes from B0012 contained in E0840, a double terminator in which B0010 is the second one. We used an extraction PCR. Released HQ 2013 This is the B0010 part extracted by PCR from the doulbe terminator B0012. We didn't manage to extract it directly from the registry and we discovered that others had the same problem; for this reason we are submitting it once again, confirmed by sequencing and by experience...ready to use! The characterization has been done both with T7 and Coli RNApolymerases. false false _977_ 0 12063 9 In stock true I don't have any design considerations, anyway it is useful to use TBE1X instead of TAE1X to make screening gels of such small fragments. false Anna Depetris, Giacomo Giacomelli annotation2180631 1 B0010 range2180631 1 1 79 BBa_K731710 1 BBa_K731710 Platform for terminators analysis under the control of tac promoter 2012-08-14T11:00:00Z 2015-05-08T01:13:06Z The Ptac promoter has been inserted with a PCR, the sequence of the promoter has been taken from literature, while the original plamid is pET21b This is the twin construct of BBa_K731700, with the same two subsequent fluorescent proteins, with T7 promoter replaced with Ptac. This construct allows to test possible differences in terminator efficiency due to different promoter and thus polymerase. We tested both constructs in the E.Coli strain BL21pLysS, that has both polymerases. false false _977_ 0 12063 9 It's complicated true The presence of the promoter cannot be tested with screening digestion, as it has more or less the same T7 promoter`s length. Anyway the screening in amplification strains is much more easy: the right colonies will be intensely red. false Giacomo Giacomelli, Anna Depetris annotation2179568 1 mCHERRY range2179568 1 6134 6844 annotation2179565 1 lacI range2179565 1 4561 5653 annotation2179566 1 Ptac range2179566 1 6033 6061 annotation2179555 1 A206K VENUS range2179555 1 36 752 annotation2179563 1 T7 TERMINATOR range2179563 1 843 890 annotation2179564 1 ampicillin resistance gene range2179564 1 1711 2370 annotation2179567 1 lacO range2179567 1 6065 6087 BBa_K731712_sequence 1 tactagtagcggccgctgcagaaggagaataatctatgagcaaaggcgaagaactgttcacgggtgtggttccgatcctggttgaactggatggcgatgtgaacggtcataaatttagcgtgtctggtgaaggcgaaggtgatgcgacctacggcaaactgacgctgaaactgatttgcaccacgggtaaactgccggttccgtggccgaccctggtgaccacgctgggttatggtctgatgtgtttcgcacgttacccggatcacatgaaacgccatgatttctttaaatctgcgatgccggaaggctatgtgcaggaacgtaccatctttttcaaagatgatggtaactacaaaacccgcgcggaagttaaatttgaaggcgatacgctggtgaaccgtattgaactgaaaggtatcgatttcaaagaagatggcaatattctgggtcacaaactggaatacaactacaacagtcataacgtgtacattaccgccgataaacagaaaaacggtatcaaagcaaacttcaaaatccgtcacaacatcgaagatggcggtgttcagctggccgatcattaccagcagaacaccccgattggcgatggtccggtgctgctgccggataatcattatctgagttaccagagcaaactgtctaaagatccgaatgaaaaacgcgatcacatggttctgctggaatttgtgaccgcggccggcattacgcatggtatggatgaactgtataaataactcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggatggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaatgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgcccggttactggaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttccacagggtagccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagactttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatctcgatcccgcgaaatgttgacaattaatcatcggctcgtataatgtgtgggaattgtgagcggataacaattcccctcaagaaataattttgtttaactttaagaaggagatatacatatggtgagtaaaggcgaggaggacaatatggcgatcatcaaagagttcatgcgcttcaaagtccacatggaaggcagcgttaatggtcacgagttcgaaattgagggcgaaggcgaaggtcgtccgtatgagggtacacagaccgctaaactgaaagtcacgaaaggtggtccactgccatttgcttgggatattctgagcccacagttcatgtatggctccaaagcctatgtgaaacatccggccgatattccggactatctgaaactgagcttccctgaagggttcaaatgggaacgtgtgatgaactttgaggatggtggtgttgtgacagtgacacaggattctagcctgcaagacggtgagttcatctataaagtgaaactgcgtggcacgaattttccgagtgatggcccggttatgcagaaaaaaacgatgggttgggaggcctctagtgagcgtatgtatccagaagatggcgctctgaaaggcgaaatcaaacagcgtctgaaactgaaagatggtggccactatgatgccgaagtgaaaaccacgtataaagccaaaaaacctgtccaactgcctggtgcctataacgttaacatcaaactggacatcacctcacacaatgaggactatacgatcgtggagcagtatgagcgtgctgaaggacgtcattctaccggtggtatggatgagctgtataaataagcggatcctaattcaattaggaattcgcggccgcttctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct BBa_K731722_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct BBa_K731710_sequence 1 tactagtagcggccgctgcagaaggagaataatctatgagcaaaggcgaagaactgttcacgggtgtggttccgatcctggttgaactggatggcgatgtgaacggtcataaatttagcgtgtctggtgaaggcgaaggtgatgcgacctacggcaaactgacgctgaaactgatttgcaccacgggtaaactgccggttccgtggccgaccctggtgaccacgctgggttatggtctgatgtgtttcgcacgttacccggatcacatgaaacgccatgatttctttaaatctgcgatgccggaaggctatgtgcaggaacgtaccatctttttcaaagatgatggtaactacaaaacccgcgcggaagttaaatttgaaggcgatacgctggtgaaccgtattgaactgaaaggtatcgatttcaaagaagatggcaatattctgggtcacaaactggaatacaactacaacagtcataacgtgtacattaccgccgataaacagaaaaacggtatcaaagcaaacttcaaaatccgtcacaacatcgaagatggcggtgttcagctggccgatcattaccagcagaacaccccgattggcgatggtccggtgctgctgccggataatcattatctgagttaccagagcaaactgtctaaagatccgaatgaaaaacgcgatcacatggttctgctggaatttgtgaccgcggccggcattacgcatggtatggatgaactgtataaataactcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggatggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaatgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgcccggttactggaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttccacagggtagccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagactttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatctcgatcccgcgaaatgttgacaattaatcatcggctcgtataatgtgtgggaattgtgagcggataacaattcccctcaagaaataattttgtttaactttaagaaggagatatacatatggtgagtaaaggcgaggaggacaatatggcgatcatcaaagagttcatgcgcttcaaagtccacatggaaggcagcgttaatggtcacgagttcgaaattgagggcgaaggcgaaggtcgtccgtatgagggtacacagaccgctaaactgaaagtcacgaaaggtggtccactgccatttgcttgggatattctgagcccacagttcatgtatggctccaaagcctatgtgaaacatccggccgatattccggactatctgaaactgagcttccctgaagggttcaaatgggaacgtgtgatgaactttgaggatggtggtgttgtgacagtgacacaggattctagcctgcaagacggtgagttcatctataaagtgaaactgcgtggcacgaattttccgagtgatggcccggttatgcagaaaaaaacgatgggttgggaggcctctagtgagcgtatgtatccagaagatggcgctctgaaaggcgaaatcaaacagcgtctgaaactgaaagatggtggccactatgatgccgaagtgaaaaccacgtataaagccaaaaaacctgtccaactgcctggtgcctataacgttaacatcaaactggacatcacctcacacaatgaggactatacgatcgtggagcagtatgagcgtgctgaaggacgtcattctaccggtggtatggatgagctgtataaataagcggatcctaattcaattaggaattcgcggccgcttctagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z