BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K732001
1
synCG
Synthetic Chorionic Gonadotropin, beta (synCG-β)
2012-09-30T11:00:00Z
2015-05-08T01:13:06Z
The sequence of hCG-beta in the human genome has been known since 1980. Starting from a consensus protein sequence on NCBI, we codon-optimized the sequence using GeneDesigner.
A synthetic version of human chorionic gonadotropin (hCG), a hormone produced during pregnancy. The nucleotide sequence was modified to maximize expression efficiency in bacteria and to conform to standard assembly, and the gene was chemically synthesized. The protein coded by this part is the beta subunit of hCG (hCG-β), which contains epitopes recognized by pregnancy tests. The full hCG dimer is also used as an ovulation inducer and can stimulate the production of testosterone in men.
This sequence can be used to express hCG for any downstream uses, and also as an output signal since it triggers pregnancy tests at low concentrations when properly folded.
false
false
_978_
0
9227
9
It's complicated
false
Started with NCBI???s protein consensus sequence, and then codon-optimized for expression in Bordetella subspecies, removed all illegal and strongly-preferred-absent cut sites, and corrected for hairpins and GC content. During the design process, we codon-optimized for several Bordetella subspecies and E. coli using GeneDesigner codon tables and noted that the resulting sequences were highly conserved. For ease of synthesis, synonymous codons of slightly lower usage bias were used in some cases to reduce GC content and avoid hairpin structures.
false
Joshua Fass
BBa_K732002
1
BBa_K732002
synCG-β + Promoter + RBS
2012-09-30T11:00:00Z
2015-05-08T01:13:06Z
Three BioBricks, one of which is from T7 bacteriophage, another of which is derived from a human gene.
This construct contains a strong constitutive promoter (J23119) as well as an RBS (B0034) upstream of synCG-β (K732001), and should strongly express a synthetic version of the beta subunit of human chorionic gonadotropin.
false
false
_978_
0
9227
9
It's complicated
false
The promoter and RBS will drive and enable efficient expression of the synthetic coding region.
false
Joshua Fass
component2205268
1
BBa_K732001
component2205265
1
BBa_J23119
component2205267
1
BBa_B0034
annotation2205267
1
BBa_B0034
range2205267
1
44
55
annotation2205268
1
BBa_K732001
range2205268
1
62
559
annotation2205265
1
BBa_J23119
range2205265
1
1
35
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K732002_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatggagatgttccagggcctgctgctgctgctgctgctgagcatgggcggcacctgggccagcaaggagccgctgcgcccgcgctgccgcccgatcaacgccaccctggccgtggagaaggagggctgcccggtgtgcatcaccgtgaacaccaccatctgcgccggctactgcccgaccatgacccgcgtgctccagggcgtgctgccggccctgccgcaggtggtgtgcaactaccgcgacgtgcgcttcgagagcatccgcctgccgggctgcccgcgcggcgtgaacccggtggtgagctacgccgtggccctgtcgtgccagtgcgccctgtgtcgtcgttcgaccaccgactgcggcggcccgaaggaccacccgttgacctgcgacgacccgcgtttccaggacagcagcagcagcaaggccccgccgccgagcctgccgagcccgagccgtttgccgggcccgagcgacaccccgattctgccgcagtga
BBa_K732001_sequence
1
atggagatgttccagggcctgctgctgctgctgctgctgagcatgggcggcacctgggccagcaaggagccgctgcgcccgcgctgccgcccgatcaacgccaccctggccgtggagaaggagggctgcccggtgtgcatcaccgtgaacaccaccatctgcgccggctactgcccgaccatgacccgcgtgctccagggcgtgctgccggccctgccgcaggtggtgtgcaactaccgcgacgtgcgcttcgagagcatccgcctgccgggctgcccgcgcggcgtgaacccggtggtgagctacgccgtggccctgtcgtgccagtgcgccctgtgtcgtcgttcgaccaccgactgcggcggcccgaaggaccacccgttgacctgcgacgacccgcgtttccaggacagcagcagcagcaaggccccgccgccgagcctgccgagcccgagccgtttgccgggcccgagcgacaccccgattctgccgcagtga
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z