LOCUS       BBa_K733007             2070 bp    DNA     linear       22-Nov-2024
DEFINITION  <i>Pveg</i> + spoVG RBS + <i>lytC</i> + linker + RPMrel + 
            consensus RBS + GFP + double terminator
ACCESSION   BBa_K733007
VERSION     BBa_K733007.1  
FEATURES             Location/Qualifiers
     promoter        1..97
                     /label=pVeg
     misc_feature    98..105
                     /label=scar
     RBS             106..120
                     /label=synthetic RBS
     misc_feature    121..126
                     /label=scar
     CDS             127..1080
                     /label=lytC cell wall domain coding region
     CDS             1081..1158
                     /label=helical linker
     misc_feature    1159..1185
                     /label=RPMrel peptide
     misc_feature    1186..1191
                     /label=double stop codons
     misc_feature    1192..1199
                     /label=scar
     CDS             1214..1927
                     /label=GFPmut3b
     misc_feature    1928..1933
                     /label=double stop codons
     misc_feature    1934..1941
                     /label=scar
     misc_feature    1942..2070
                     /label=double terminator
     RBS             1200..1210
                     /label=consensus RBS
ORIGIN
        1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt
       61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagtcttc agaaaggagt
      121 tactagatgc gttcttatat aaaagtccta acaatgtgtt ttctggggct catacttttt
      181 gtgccaacag ctttggccga taactcagtg aaaagagttg ggggaagcaa tagatacggc
      241 actgctgtac aaatatcaaa gcaaatgtat tcaacagcaa gtacagctgt aattgttggt
      301 gggagttcct atgcagatgc tatttcagca gcacctcttg cttaccagaa gaatgcgcca
      361 ttgctttaca ctaattctga taagctttca tatgaaacga aaacaagatt gaaagagatg
      421 cagactaaaa atgtaattat tgtaggcgga acacctgctg tttcttctaa cactgctaac
      481 cagattaaaa gcttggggat aagtattaaa cgaattgcag gaagcaaccg ttatgatacg
      541 gctgcacggg tggcaaaagc gatgggtgcg acttcaaaag ctgttatttt gaacggcttc
      601 ttatatgcag acgctccggc cgtcatccct tatgcagcga aaaacgggta tccaattctt
      661 tttacaaata aaacatctat aaatagtgcg actacgtctg tgataaaaga taagggaatt
      721 tcgagtaccg ttgttgtagg aggcactgga agtatcagca atacggtata caacaagtta
      781 ccttctccta caagaattag cggttcaaac agatatgagc ttgctgcaaa tatcgtacaa
      841 aaacttaatt tatcaacaag caccgtatat gtaagcaatg gattcagcta ccctgactct
      901 attgcaggag ctacactggc agctaagaag aagcaatctc ttattcttac aaatggtgaa
      961 aatttatcta caggagcccg taaaattatt ggaagtaaaa acatgtcaaa ctttatgatt
     1021 atcggaaaca ctcctgccgt aagcacaaag gttgctaatc agctaaagaa tccagttgta
     1081 agcagaggct cacgcgcact ggcagaagcc gcagctaagg aagctgctgc aaaagaggcg
     1141 gcggcgaaag ccgcggcctg tccgatcgaa gatcgcccga tgtgttaata atactagaga
     1201 aaggaggtgt tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt
     1261 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
     1321 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
     1381 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
     1441 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
     1501 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
     1561 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
     1621 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
     1681 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
     1741 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
     1801 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
     1861 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
     1921 atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1981 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     2041 caccttcggg tgggcctttc tgcgtttata
//