LOCUS       BBa_K733009              949 bp    DNA     linear       22-Nov-2024
DEFINITION  <i>Ptms</i>+BBa_E0240: <i>Ptms</i>+RBS+GFP+Double
ACCESSION   BBa_K733009
VERSION     BBa_K733009.1  
FEATURES             Location/Qualifiers
     promoter        14..20
                     /label=Sigma A binding sequence, -35
     promoter        40..46
                     /label=Sigma A binding sequence, -10
     promoter        1..65
                     /label=BBa_K733001
     RBS             74..86
                     /label=RBS-3\Weak
     RBS             80..83
                     /label=RBS
     misc_feature    74..86
                     /label=BBa_B0032
     RBS             74..86
                     /label=BBa_B0032
     CDS             93..812
                     /label=GFP protein
     CDS             93..812
                     /label=BBa_E0040
     stem_loop       832..875
                     /label=stem_loop
     misc_feature    821..900
                     /label=BBa_B0010
     terminator      821..900
                     /label=BBa_B0010
     stem_loop       916..935
                     /label=T7 TE
     misc_feature    942..942
                     /label=stop
     polyA_site      936..949
                     /label=polya
     misc_feature    909..949
                     /label=BBa_B0012
     terminator      909..949
                     /label=BBa_B0012
     misc_feature    74..949
                     /label=BBa_E0240
ORIGIN
        1 catgaagtct ccttgaaatc agaagatatt taggatatat ttttctatgg ataaaaggga
       61 tattgtacta gagtcacaca ggaaagtact agatgcgtaa aggagaagaa cttttcactg
      121 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
      181 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
      241 ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat
      301 gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg
      361 aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg
      421 ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt
      481 ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac tcacacaatg
      541 tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca
      601 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg
      661 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag
      721 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta
      781 cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga
      841 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      901 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//