LOCUS       BBa_K733017              693 bp    DNA     linear       25-Nov-2024
DEFINITION  <i>Pveg</i>+RBS+<i>ybdN</i>+<i>Bmp2</i>+terminator
ACCESSION   BBa_K733017
VERSION     BBa_K733017.1  
FEATURES             Location/Qualifiers
     promoter        63..68
                     /label=Sigma A-35
     promoter        86..91
                     /label=Sigma A-35
     promoter        1..97
                     /label=BBa_K316001
     RBS             98..109
                     /label=rbs
     RBS             98..109
                     /label=BBa_K143021
     promoter        1..109
                     /label=BBa_K733013
     CDS             118..208
                     /label=Signal Peptide, YbdN
     CDS             209..556
                     /label=Mature BMP-2
     CDS             118..556
                     /label=BBa_K733005
     misc_feature    1..556
                     /label=BBa_K733015
     stem_loop       576..619
                     /label=stem_loop
     misc_feature    565..644
                     /label=BBa_B0010
     terminator      565..644
                     /label=BBa_B0010
     stem_loop       660..679
                     /label=T7 TE
     misc_feature    686..686
                     /label=stop
     polyA_site      680..693
                     /label=polya
     misc_feature    653..693
                     /label=BBa_B0012
     terminator      653..693
                     /label=BBa_B0012
     terminator      565..693
                     /label=BBa_B0015
ORIGIN
        1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt
       61 atttgacaaa aatgggctcg tgttgtacaa taaatgtaaa ggtggtgaat actagagaaa
      121 ggaggttgtt tgcatggtga aaaaatggct tattcaattc gctgttatgc tttctgtctt
      181 atctaccttt acgtattcgg catcagctca agccaaacac aaacagcgga agcgcctcaa
      241 gtccagctgc aagagacacc ctttgtatgt ggacttcagt gatgtggggt ggaatgactg
      301 gatcgtggca cctccgggct atcatgcctt ttactgccat ggggagtgtc cttttcccct
      361 tgctgaccac ctgaactcca ctaaccatgc catagtgcag actctggtga actctgtgaa
      421 ctccaaaatc cctaaggcat gctgtgtccc cacagagctc agcgcaatct ccatgttgta
      481 cctagatgaa aatgaaaagg ttgtgctaaa aaattatcag gacatggttg tggagggctg
      541 cgggtgtcgt taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
      601 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      661 gctcaccttc gggtgggcct ttctgcgttt ata
//