LOCUS       BBa_K733018             2271 bp    DNA     linear       26-Dec-2024
DEFINITION  <i>xylR</i>+<i>PxylA</i>+RBS+GFP+Double Terminator
ACCESSION   BBa_K733018
VERSION     BBa_K733018.1  
FEATURES             Location/Qualifiers
     CDS             7..1173
                     /label=xylR
     promoter        1300..1305
                     /label=Sigma A, -10
     promoter        1277..1282
                     /label=Sigma A, -35
     promoter        1..1387
                     /label=BBa_K733002
     RBS             1396..1408
                     /label=RBS-3\Weak
     RBS             1402..1405
                     /label=RBS
     misc_feature    1396..1408
                     /label=BBa_B0032
     RBS             1396..1408
                     /label=BBa_B0032
     CDS             1415..2134
                     /label=GFP protein
     CDS             1415..2134
                     /label=BBa_E0040
     stem_loop       2154..2197
                     /label=stem_loop
     misc_feature    2143..2222
                     /label=BBa_B0010
     terminator      2143..2222
                     /label=BBa_B0010
     stem_loop       2238..2257
                     /label=T7 TE
     misc_feature    2264..2264
                     /label=stop
     polyA_site      2258..2271
                     /label=polya
     misc_feature    2231..2271
                     /label=BBa_B0012
     terminator      2231..2271
                     /label=BBa_B0012
     misc_feature    1396..2271
                     /label=BBa_E0240
ORIGIN
        1 gagctcctaa cttatagggg taacacttaa aaaagaatca ataacgatag aaaccgctcc
       61 taaagcaggt gcattttttc ctaacgaaga aggcaatagt tcacatttat tgtctaaatg
      121 agaatggact cttgaagaaa cttcgttttt aatcgtattt aaaacaatgg gatgagattc
      181 aattatatga tttctcaaga taacagcttc tatatcaaat gtattaagga tattggttaa
      241 tccaattccg atataaaagc caaagttttg aagtgcattt aacatttcta catcattttt
      301 atttgcgcgt tccacaatct cttttcgaga aatattcttt tcttctttag agagcgaagc
      361 cagtaacgct ttttcagaag catataattc ccaacagcct cgatttccac agctgcattt
      421 gggtccatta aaatctatcg tcatatgacc catttcccca gaaaaaccct gaacaccttt
      481 atacaattcg ttgttaataa caagtccagt tccaattccg atattaatac tgatgtaaac
      541 gatgttttca tagttttttg tcataccaaa tactttttca ccgtatgctc ctgcattagc
      601 ttcattttca acaaaaaccg gaacattaaa ctcactctca attaaaaact gcaaatcttt
      661 gatattccaa tttaagttag gcatgaaaat aatttgctga tgacgatcta caaggcctgg
      721 aacacaaatt cctattccga ctagaccata aggggactca ggcatatggg ttacaaaacc
      781 atgaataagt gcaaataaaa tctcttttac ttcactagcg gaagaactag acaagtcaga
      841 agtcttctcg agaataatat ttccttctaa gtcggttaga atgccgttaa gatagtcgac
      901 tcctatatca ataccaatcg agtagcctgc attcttatta aaaacaagca ttacaggtct
      961 tctgccgcct cttgattgcc ctgccccaat ttcaaaaata aaatcttttt caagcagtgt
     1021 atttacttga gaggagacag tagacttgtt taatcctgta atctcagaga gagttgccct
     1081 ggagacaggg gagttcttca aaatttcatc taatattaat ttttgattca ttttttttac
     1141 taaagcttga tctgcaattt gaataataac cactcctttg tttatccacc gaactaagtt
     1201 ggtgtttttt gaagcttgaa ttagatattt aaaagtatca tatctaatat tataactaaa
     1261 ttttctaaaa aaaacattga aataaacatt tattttgtat atgatgagat aaagttagtt
     1321 tattggataa acaaactaac tcaattaaga tagttgatgg ataaacttgt tcacttaaat
     1381 caaagggtac tagagtcaca caggaaagta ctagatgcgt aaaggagaag aacttttcac
     1441 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
     1501 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
     1561 tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca
     1621 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc
     1681 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
     1741 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
     1801 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa
     1861 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca
     1921 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg
     1981 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
     2041 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
     2101 tacacatggc atggatgaac tatacaaata ataatactag agccaggcat caaataaaac
     2161 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     2221 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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