LOCUS BBa_K733018 2271 bp DNA linear 03-May-2024
DEFINITION xylR+PxylA+RBS+GFP+Double Terminator
ACCESSION BBa_K733018
VERSION BBa_K733018.1
FEATURES Location/Qualifiers
CDS 7..1173
/label=xylR
promoter 1300..1305
/label=Sigma A, -10
promoter 1277..1282
/label=Sigma A, -35
promoter 1..1387
/label=BBa_K733002
RBS 1396..1408
/label=RBS-3\Weak
RBS 1402..1405
/label=RBS
misc_feature 1396..1408
/label=BBa_B0032
RBS 1396..1408
/label=BBa_B0032
CDS 1415..2134
/label=GFP protein
CDS 1415..2134
/label=BBa_E0040
stem_loop 2154..2197
/label=stem_loop
misc_feature 2143..2222
/label=BBa_B0010
terminator 2143..2222
/label=BBa_B0010
stem_loop 2238..2257
/label=T7 TE
misc_feature 2264..2264
/label=stop
polyA_site 2258..2271
/label=polya
misc_feature 2231..2271
/label=BBa_B0012
terminator 2231..2271
/label=BBa_B0012
misc_feature 1396..2271
/label=BBa_E0240
ORIGIN
1 gagctcctaa cttatagggg taacacttaa aaaagaatca ataacgatag aaaccgctcc
61 taaagcaggt gcattttttc ctaacgaaga aggcaatagt tcacatttat tgtctaaatg
121 agaatggact cttgaagaaa cttcgttttt aatcgtattt aaaacaatgg gatgagattc
181 aattatatga tttctcaaga taacagcttc tatatcaaat gtattaagga tattggttaa
241 tccaattccg atataaaagc caaagttttg aagtgcattt aacatttcta catcattttt
301 atttgcgcgt tccacaatct cttttcgaga aatattcttt tcttctttag agagcgaagc
361 cagtaacgct ttttcagaag catataattc ccaacagcct cgatttccac agctgcattt
421 gggtccatta aaatctatcg tcatatgacc catttcccca gaaaaaccct gaacaccttt
481 atacaattcg ttgttaataa caagtccagt tccaattccg atattaatac tgatgtaaac
541 gatgttttca tagttttttg tcataccaaa tactttttca ccgtatgctc ctgcattagc
601 ttcattttca acaaaaaccg gaacattaaa ctcactctca attaaaaact gcaaatcttt
661 gatattccaa tttaagttag gcatgaaaat aatttgctga tgacgatcta caaggcctgg
721 aacacaaatt cctattccga ctagaccata aggggactca ggcatatggg ttacaaaacc
781 atgaataagt gcaaataaaa tctcttttac ttcactagcg gaagaactag acaagtcaga
841 agtcttctcg agaataatat ttccttctaa gtcggttaga atgccgttaa gatagtcgac
901 tcctatatca ataccaatcg agtagcctgc attcttatta aaaacaagca ttacaggtct
961 tctgccgcct cttgattgcc ctgccccaat ttcaaaaata aaatcttttt caagcagtgt
1021 atttacttga gaggagacag tagacttgtt taatcctgta atctcagaga gagttgccct
1081 ggagacaggg gagttcttca aaatttcatc taatattaat ttttgattca ttttttttac
1141 taaagcttga tctgcaattt gaataataac cactcctttg tttatccacc gaactaagtt
1201 ggtgtttttt gaagcttgaa ttagatattt aaaagtatca tatctaatat tataactaaa
1261 ttttctaaaa aaaacattga aataaacatt tattttgtat atgatgagat aaagttagtt
1321 tattggataa acaaactaac tcaattaaga tagttgatgg ataaacttgt tcacttaaat
1381 caaagggtac tagagtcaca caggaaagta ctagatgcgt aaaggagaag aacttttcac
1441 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
1501 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
1561 tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca
1621 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc
1681 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
1741 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
1801 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa
1861 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca
1921 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg
1981 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
2041 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
2101 tacacatggc atggatgaac tatacaaata ataatactag agccaggcat caaataaaac
2161 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
2221 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//