LOCUS BBa_K734003 6130 bp DNA linear 26-Nov-2024 DEFINITION CRE regulated by LasR quorum sensing pathway ACCESSION BBa_K734003 VERSION BBa_K734003.1 FEATURES Location/Qualifiers misc_feature 1310..1429 /label=BBa_B0015 misc_feature 1469..2224 /label=BBa_C0079 promoter 2236..2304 /label=LacUV5 misc_feature 2355..2511 /label=BBa_R0079 CDS 2523..3554 /label=CRE misc_feature 3555..4054 /label=Terminator misc_feature 4056..6130 /label=pACYC Backbone misc_feature 1..1309 /label=pACYC Backbone ORIGIN 1 tgtagcacct gaagtcagcc ccatacgata taagttgtaa ttctcatgtt agtcatgccc 61 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc 121 ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag 181 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 241 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt 301 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag 361 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt 421 atcgtcgtat cccactaccg agatgtccgc accaacgcgc agcccggact cggtaatggc 481 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc 541 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg 601 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag 661 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc 721 gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat 781 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac 841 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc 901 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac 961 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg 1021 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag 1081 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc 1141 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga 1201 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa 1261 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgct ataaacgcag 1321 aaaggcccac ccgaaggtga gccagtgtga ctctagtaga gagcgttcac cgacaaacaa 1381 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggct 1441 cgaaccattg ccaagccaac gaacagtttt aagctactaa agcgtagttt tcgtcgtttg 1501 cagcttagag agtaataaga cccaaattaa cggccataat ggccgctacg cggcgggagg 1561 tcacaccgaa cttccgccga atatttccca tatggaagtt cacattggct tccgagcagt 1621 tgcagataac cgatatctcc caactggtct tgccgatggc gcaccactgc aacacttcct 1681 tctcccggct ggtcagaacc accggtttgc tgaccggatg ttcgaaggcc agtccggcac 1741 cgctttgcag tgcgtagtcc ttgagcatcc acagggtcgg caggaccgac tctatgaaac 1801 ggttggcctc ggcccggttt tccgcttcca cgctgaggct cagcgcgccg agttcgccgc 1861 gagcaccatg cagcggcatg gtcagcccat acaccaggcc ggcggccgag gcttcctcga 1921 agaactcgtg ctgctttcgc gtctggtaga tggacggttc ccagaaaatc ggcagtacgc 1981 tctgggtaca gtgactgacc gtcgggtcga cccgcgcgta gccagcccgg tcgtaatgct 2041 cgcgccaggc ggccgggtag ttgccgacga tgaaggcgtt ctcgtagtcc tggctgtcct 2101 taggcaacag gccgaacagg atcttcgaga atccaaggtc gctcgccatc ttctggagga 2161 tggcgctcca ctccaatttt ccacttgagc gttccagctc aagaaaaccg tcaaccaagg 2221 ccatatgtgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacat tatacgagcc 2281 ggaagcataa agtgtaaagc ctggcattcg atggtgtccg ggatctcgac gctctccctt 2341 atgcgactcc tgccgcccct cgctgagcgc gtcccggagc tgggggcaac ctagctgcca 2401 cctgcttttc tgctagctat tccagcgaaa acatacagat ttccggcgaa atcaaggcta 2461 cctgccagtt ctggcaggtt tggccgcggg ttctttttgg tacacgaaag caggactact 2521 agatgtccaa tttactgacc gtacaccaaa atttgcctgc attaccggtc gatgcaacga 2581 gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg ttttctgagc 2641 atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc aagttgaata 2701 accggaaatg gtttcccgca gaacctgaag atgttcgcga ttatcttcta tatcttcagg 2761 cgcgcggtct ggcagtaaaa actatccagc aacatttggg ccagctaaac atgcttcatc 2821 gtcggtccgg gctgccacga ccaagtgaca gcaatgctgt ttcactggtt atgcggcgga 2881 tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg ttcgaacgca 2941 ctgatttcga ccaggttcgt tcactcatgg aaaatagcga tcgctgccag gatatacgta 3001 atctggcatt tctggggatt gcttataaca ccctgttacg tatagccgaa attgccagga 3061 tcagggttaa agatatctca cgtactgacg gtgggagaat gttaatccat attggcagaa 3121 cgaaaacgct ggttagcacc gcaggtgtag agaaggcact tagcctgggg gtaactaaac 3181 tggtcgagcg atggatttcc gtctctggtg tagctgatga tccgaataac tacctgtttt 3241 gccgggtcag aaaaaatggt gttgccgcgc catctgccac cagccagcta tcaactcgcg 3301 ccctggaagg gatttttgaa gcaactcatc gattgattta cggcgctaag gatgactctg 3361 gtcagagata cctggcctgg tctggacaca gtgcccgtgt cggagccgcg cgagatatgg 3421 cccgcgctgg agtttcaata ccggagatca tgcaagctgg tggctggacc aatgtaaata 3481 ttgtcatgaa ctatatccgt aacctggata gtgaaacagg ggcaatggtg cgcctgctgg 3541 aagatggcgc ttagcccggg aagccgaatt cgagctcggt acccagttcg tcgatgggtt 3601 cgggaatgca ggatattccc acctccggtt aaggatggaa gagagtatct gttccacgaa 3661 tcagcggtaa aggttgactt aaatcgacca gtaacaggtg gccttttgaa gaggatcaga 3721 aatgggaaga aggcgaagtc atgagcgccg ggatttaccc cctaaccttt atataagaaa 3781 caatggatat tactgctaca gggacccaag gacgggtaaa gagtttggat taggcagaga 3841 caggcgaatc gcaatcactg aagctataca ggccaacatt gagttatttt caggacacaa 3901 acacaagcct ctgacagcga gaatcaacag tgataattcc gttacgttac attcatggct 3961 tgatcgctac gaaaaaatcc tggccagcag aggaatcaag cagaagacac tcataaatta 4021 catgagcaaa attaaagcaa taaggagggg tctgactgct tccggtagtc aataaaccgg 4081 taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt 4141 cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca 4201 accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc 4261 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc 4321 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc 4381 catagtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt 4441 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa 4501 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg 4561 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac 4621 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg 4681 gaactccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt 4741 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt 4801 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga 4861 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga 4921 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt 4981 ggaacctctt acgtgccgat cacgtctcat tttcgccaaa gttggccagg gcttcccggt 5041 atcaacaggg acaccaggat ttatttattg cgaagtgatc ttccgtcaca ggtatttatt 5101 cggcgcaaag tgcgtcgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt 5161 tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg 5221 gtggtgcgta acggcaaaag caccgccgga catcagcgct agcggagtgt atactggctt 5281 actatgttgg cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc 5341 tgcaccggtg cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga 5401 ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga 5461 tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg 5521 tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg 5581 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg 5641 cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg 5701 tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat 5761 gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 5821 caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg 5881 agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact 5941 gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa 6001 aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac 6061 gatctcaaga agatcatctt attaatcaga taaaatattt ctagatttca gtgcaattta 6121 tctcttcaaa //