LOCUS BBa_K734004 5516 bp DNA linear 25-Nov-2024 DEFINITION sfGFP regulated by LasR quorum sensing pathway ACCESSION BBa_K734004 VERSION BBa_K734004.1 FEATURES Location/Qualifiers misc_feature 1469..2224 /label=BBa_C0079 misc_feature 1310..1429 /label=BBa_B0015 misc_feature 2355..2511 /label=BBa_R0079 promoter 2236..2304 /label=LacUV5 CDS 2523..3252 /label=sfGFP misc_feature 3283..3440 /label=rrnB Terminator misc_feature 3440..5516 /label=pACYC Backbone misc_feature 1..1309 /label=pACYC Backbone ORIGIN 1 tgtagcacct gaagtcagcc ccatacgata taagttgtaa ttctcatgtt agtcatgccc 61 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc 121 ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag 181 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 241 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt 301 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag 361 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt 421 atcgtcgtat cccactaccg agatgtccgc accaacgcgc agcccggact cggtaatggc 481 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc 541 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg 601 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag 661 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc 721 gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat 781 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac 841 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc 901 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac 961 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg 1021 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag 1081 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc 1141 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga 1201 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa 1261 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgct ataaacgcag 1321 aaaggcccac ccgaaggtga gccagtgtga ctctagtaga gagcgttcac cgacaaacaa 1381 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggct 1441 cgaaccattg ccaagccaac gaacagtttt aagctactaa agcgtagttt tcgtcgtttg 1501 cagcttagag agtaataaga cccaaattaa cggccataat ggccgctacg cggcgggagg 1561 tcacaccgaa cttccgccga atatttccca tatggaagtt cacattggct tccgagcagt 1621 tgcagataac cgatatctcc caactggtct tgccgatggc gcaccactgc aacacttcct 1681 tctcccggct ggtcagaacc accggtttgc tgaccggatg ttcgaaggcc agtccggcac 1741 cgctttgcag tgcgtagtcc ttgagcatcc acagggtcgg caggaccgac tctatgaaac 1801 ggttggcctc ggcccggttt tccgcttcca cgctgaggct cagcgcgccg agttcgccgc 1861 gagcaccatg cagcggcatg gtcagcccat acaccaggcc ggcggccgag gcttcctcga 1921 agaactcgtg ctgctttcgc gtctggtaga tggacggttc ccagaaaatc ggcagtacgc 1981 tctgggtaca gtgactgacc gtcgggtcga cccgcgcgta gccagcccgg tcgtaatgct 2041 cgcgccaggc ggccgggtag ttgccgacga tgaaggcgtt ctcgtagtcc tggctgtcct 2101 taggcaacag gccgaacagg atcttcgaga atccaaggtc gctcgccatc ttctggagga 2161 tggcgctcca ctccaatttt ccacttgagc gttccagctc aagaaaaccg tcaaccaagg 2221 ccatatgtgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacat tatacgagcc 2281 ggaagcataa agtgtaaagc ctggcattcg atggtgtccg ggatctcgac gctctccctt 2341 atgcgactcc tgccgcccct cgctgagcgc gtcccggagc tgggggcaac ctagctgcca 2401 cctgcttttc tgctagctat tccagcgaaa acatacagat ttccggcgaa atcaaggcta 2461 cctgccagtt ctggcaggtt tggccgcggg ttctttttgg tacacgaaag caggactact 2521 agatggcatc caagggcgag gagctcttta ctggcgtagt accaattctc gtagagctcg 2581 atggcgatgt aaatggccat aagttttccg tacgcggcga gggcgagggc gatgcaacta 2641 acggcaagct cactctcaag tttatttgta ctactggcaa gctcccagta ccatggccaa 2701 ctctcgtaac tactctgacc tatggcgtac aatgtttttc ccgctatcca gatcacatga 2761 agcaacatga tttttttaag tccgcaatgc cagagggcta tgtacaagag cgcactatta 2821 gctttaagga tgatggcacc tataagactc gcgcagaggt aaagtttgag ggcgatactc 2881 tcgtaaatcg cattgagctc aagggcattg attttaagga ggatggcaat attctcggcc 2941 ataagctgga gtataatttc aattcccata atgtatatat taccgcagat aagcaaaaga 3001 atggcattaa ggcgaatttt aagattcgcc ataatgtgga ggatggctcc gtacaactcg 3061 cagatcatta tcaacaaaat actccaattg gcgatggccc agtactcctc ccagataatc 3121 attatctctc cactcaatcc gtgctctcca aagatccaaa tgagaagcgc gatcacatgg 3181 tactcctgga gtttgtaact gcagcaggca ttactcatgg catggatgag ctctataagc 3241 tcgagatgct aagatagtgg agaggagaga gaatcgccag aatgcctggc ggcagtagcg 3301 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta 3361 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct 3421 cagtcgaaag actgggcctt actgcttccg gtagtcaata aaccggtaaa ccagcaatag 3481 acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg 3541 aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaacca ggcgtttaag 3601 ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt 3661 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa 3721 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccata gtgaaaacgg 3781 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg 3841 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt 3901 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt 3961 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt 4021 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaac tccggatgag 4081 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct 4141 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag 4201 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg 4261 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact 4321 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt 4381 gccgatcacg tctcattttc gccaaagttg gccagggctt cccggtatca acagggacac 4441 caggatttat ttattgcgaa gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg 4501 tcgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt 4561 ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg 4621 caaaagcacc gccggacatc agcgctagcg gagtgtatac tggcttacta tgttggcact 4681 gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc 4741 agcagaatat gtgatacagg atatattccg cttcctcgct cactgactcg ctacgctcgg 4801 tcgttcgact gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg 4861 ccaggaagat acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct 4921 ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac 4981 aggactataa agataccagg cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc 5041 tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc 5101 tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg 5161 ttcagtccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac 5221 atgcaaaagc accactggca gcagccactg gtaattgatt tagaggagtt agtcttgaag 5281 tcatgcgccg gttaaggcta aactgaaagg acaagttttg gtgactgcgc tcctccaagc 5341 cagttacctc ggttcaaaga gttggtagct cagagaacct tcgaaaaacc gccctgcaag 5401 gcggtttttt cgttttcaga gcaagagatt acgcgcagac caaaacgatc tcaagaagat 5461 catcttatta atcagataaa atatttctag atttcagtgc aatttatctc ttcaaa //