LOCUS BBa_K734006 7229 bp DNA linear 25-Nov-2024 DEFINITION pReporter.permaflipped control for ZombiE.coli ACCESSION BBa_K734006 VERSION BBa_K734006.1 FEATURES Location/Qualifiers misc_feature 1569..1589 /label=lacO CDS 2759..3619 /label=ampR misc_feature 2..159 /label=rrnB terminator CDS 190..795 /label=lasI RBS 796..806 /label=strong RBS CDS 837..1517 /label=rfp RBS 1518..1528 /label=strong RBS misc_feature 1529..1562 /label=mutant loxP promoter 1563..1631 /label=lacUV5 misc_feature 1632..1665 /label=mutant loxP RBS 1666..1676 /label=strong RBS CDS 1677..2406 /label=sfGFP misc_feature 2437..2565 /label=terminator promoter 2689..2717 /label=ampR promoter misc_feature 3774..4393 /label=rep_origin CDS 5808..6989 /label=lacI ORIGIN 1 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggcag ttccctactc 61 tcgcatgggg agaccccaca ctaccatcgg cgctacggcg tttcacttct gagttcggca 121 tggggtcagg tgggaccacc gcgctactgc cgccaggcag ggtcgcatct gtttctcttg 181 agcttcttgt taggaaacag ccagacgctg ttcaaccaga acaccaccgt acagagcgat 241 ctgggtttta gcgttcagtt cgatacgcag agcaacagca cgttcgatac cgattttcag 301 gtgcggaccg aaacgggaaa cgtccagacc agcacggatc atcatttttt caacaccaac 361 ggtggtaacg gtaaccaggg tctggatgtc gttctgcaag gagtaacgag ccagagcacg 421 catagcttcc agggtgcagt cggagaaacc cagggaacct ttctgaccgg agttgatagc 481 gaaacgggac agttcccaga tgtgcgggga gcacggagct tctttaccgt gcagcagttc 541 cgggaaggtg tttttcagca tgtacggacc ggtggtgtcg aagatacgcc agcaaccgaa 601 aacctgagct tccggggtgt cttcctggat cagcatgtag tacggggaca gagcgtcgta 661 accgtcgatt tccatttcgt cgataacgga aacgtcccaa cctttacgtt ctttgaaaac 721 ctgagcacgc agtttgtgca tttcacccag cagttttttg tcgaactctt cacgacgacc 781 gatctgaacg atcatatgtg tttcctagat gcttgggatt tcggtgggtt ggttgtttat 841 taagcaccgg tggagtgacg accttcagca cgttcgtact gttcaacgat ggtgtagtct 901 tcgttgtggg aggtgatgtc cagtttgatg tcggttttgt aagcacccgg cagctgaacc 961 ggttttttag ccatgtaggt ggttttaact tcagcgtcgt agtgaccacc gtctttcagt 1021 ttcagacgca ttttgatttc acctttcaga gcaccgtctt ccgggtacat acgttcggtg 1081 gaagcttccc aacccatggt ttttttctgc ataaccggac cgtcggacgg gaagttggta 1141 ccacgcagtt taactttgta gatgaactca ccgtcttgca gggaggagtc ctgggtaacg 1201 gtaacaacac caccgtcttc gaagttcata acacgttccc atttgaaacc ttccgggaag 1261 gacagtttca ggtagtccgg gatgtcagcc gggtgtttaa cgtaagcttt ggaaccgtac 1321 tggaactgcg gggacaggat gtcccaagcg aacggcagcg gaccaccttt ggtaactttc 1381 agtttagcgg tctgggtacc ttcgtacgga cgaccttcac cttcaccttc gatttcgaac 1441 tcgtgaccgt taacggaacc ttccatacga actttgaaac gcatgaactc tttgataacg 1501 tcttcggagg aagccatatg tgtttcctat aacttcgtat agcatacatt atacgaagtt 1561 atgtgtgaaa ttgttatccg ctcacaattc cacacattat acgagccgga agcataaagt 1621 gtaaagcctg gtaccgttcg tataatgtat gctatacgaa cggtaaggaa acacatatgg 1681 catccaaggg cgaggagctc tttactggcg tagtaccaat tctcgtagag ctcgatggcg 1741 atgtaaatgg ccataagttt tccgtacgcg gcgagggcga gggcgatgca actaacggca 1801 agctcactct caagtttatt tgtactactg gcaagctccc agtaccatgg ccaactctcg 1861 taactactct gacctatggc gtacaatgtt tttcccgcta tccagatcac atgaagcaac 1921 atgatttttt taagtccgca atgccagagg gctatgtaca agagcgcact attagcttta 1981 aggatgatgg cacctataag actcgcgcag aggtaaagtt tgagggcgat actctcgtaa 2041 atcgcattga gctcaagggc attgatttta aggaggatgg caatattctc ggccataagc 2101 tggagtataa tttcaattcc cataatgtat atattaccgc agataagcaa aagaatggca 2161 ttaaggcgaa ttttaagatt cgccataatg tggaggatgg ctccgtacaa ctcgcagatc 2221 attatcaaca aaatactcca attggcgatg gcccagtact cctcccagat aatcattatc 2281 tctccactca atccgtgctc tccaaagatc caaatgagaa gcgcgatcac atggtactcc 2341 tggagtttgt aactgcagca ggcattactc atggcatgga tgagctctat aagctcgaga 2401 tgctaaaggg atgttatgcg tgttgtgagg gtttggccag gcatcaaata aaacgaaagg 2461 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 2521 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttataaaatg agctgattta 2581 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 2641 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 2701 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 2761 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 2821 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 2881 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 2941 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 3001 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 3061 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 3121 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 3181 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 3241 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 3301 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 3361 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 3421 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 3481 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 3541 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 3601 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 3661 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 3721 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 3781 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 3841 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 3901 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 3961 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 4021 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 4081 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 4141 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 4201 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 4261 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 4321 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 4381 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 4441 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 4501 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 4561 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg 4621 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat 4681 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct 4741 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 4801 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct 4861 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt 4921 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg 4981 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa 5041 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc 5101 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa 5161 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta 5221 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg 5281 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag 5341 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac 5401 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca 5461 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg 5521 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc 5581 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg 5641 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca 5701 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag 5761 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt 5821 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 5881 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc 5941 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc 6001 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct 6061 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta 6121 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg 6181 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct 6241 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga 6301 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc 6361 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg 6421 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct 6481 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt 6541 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc 6601 gacaccaccc caccgctgag caataactag cataacccct tggggcctct aaacgggtct 6661 tgaggggttt tttgctgaaa ggaggaacta tatccggata cgctggcacc cagttgatcg 6721 gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg 6781 gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg 6841 taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg 6901 gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg 6961 tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat 7021 gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt 7081 atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc 7141 cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc 7201 caccataccc acgccgaaac aagcgctca //