BBa_K734006 1 BBa_K734006 pReporter.permaflipped control for ZombiE.coli 2012-09-30T11:00:00Z 2015-05-08T01:13:07Z LasI: from pSB1A2-K081009 RFP: from pSB1AK3-K081014 double terminator: from pSB1AK3-B0015 backbone: pET21-derived This control plasmid is the ???permaflipped??? control, whose sequence is identical to that of the reporter plasmid but has the promoter flipped, as if already acted upon by Cre rcombinase. Using this control will show whether Z-Mode ("infected") cells actually express the RFP when Cre recombinase is assumed to work as intended, flipping the promoter region and generating one double-mutant and one wild-type loxP site. When transformed into cells and induced with IPTG, all cells should glow red if this control works correctly. false false _983_ 0 11676 9 Not in stock false In testing, induction with IPTG does not allow RFP expression, possibly due to extensive secondary structure on the RNA transcripts blocking the RBS. false Ben Slater annotation2205446 1 lasI range2205446 1 190 795 annotation2205455 1 terminator range2205455 1 2437 2565 annotation2205443 1 lacO range2205443 1 1569 1589 annotation2205457 1 rep_origin range2205457 1 3774 4393 annotation2205447 1 strong RBS range2205447 1 796 806 annotation2205451 1 lacUV5 range2205451 1 1563 1631 annotation2205449 1 strong RBS range2205449 1 1518 1528 annotation2205444 1 ampR range2205444 1 2759 3619 annotation2205458 1 lacI range2205458 1 5808 6989 annotation2205445 1 rrnB terminator range2205445 1 2 159 annotation2205450 1 mutant loxP range2205450 1 1529 1562 annotation2205452 1 mutant loxP range2205452 1 1632 1665 annotation2205454 1 sfGFP range2205454 1 1677 2406 annotation2205453 1 strong RBS range2205453 1 1666 1676 annotation2205448 1 rfp range2205448 1 837 1517 annotation2205456 1 ampR promoter range2205456 1 2689 2717 BBa_K734006_sequence 1 aaaggcccagtctttcgactgagcctttcgttttatttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgccaggcagggtcgcatctgtttctcttgagcttcttgttaggaaacagccagacgctgttcaaccagaacaccaccgtacagagcgatctgggttttagcgttcagttcgatacgcagagcaacagcacgttcgataccgattttcaggtgcggaccgaaacgggaaacgtccagaccagcacggatcatcattttttcaacaccaacggtggtaacggtaaccagggtctggatgtcgttctgcaaggagtaacgagccagagcacgcatagcttccagggtgcagtcggagaaacccagggaacctttctgaccggagttgatagcgaaacgggacagttcccagatgtgcggggagcacggagcttctttaccgtgcagcagttccgggaaggtgtttttcagcatgtacggaccggtggtgtcgaagatacgccagcaaccgaaaacctgagcttccggggtgtcttcctggatcagcatgtagtacggggacagagcgtcgtaaccgtcgatttccatttcgtcgataacggaaacgtcccaacctttacgttctttgaaaacctgagcacgcagtttgtgcatttcacccagcagttttttgtcgaactcttcacgacgaccgatctgaacgatcatatgtgtttcctagatgcttgggatttcggtgggttggttgtttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatatgtgtttcctataacttcgtatagcatacattatacgaagttatgtgtgaaattgttatccgctcacaattccacacattatacgagccggaagcataaagtgtaaagcctggtaccgttcgtataatgtatgctatacgaacggtaaggaaacacatatggcatccaagggcgaggagctctttactggcgtagtaccaattctcgtagagctcgatggcgatgtaaatggccataagttttccgtacgcggcgagggcgagggcgatgcaactaacggcaagctcactctcaagtttatttgtactactggcaagctcccagtaccatggccaactctcgtaactactctgacctatggcgtacaatgtttttcccgctatccagatcacatgaagcaacatgatttttttaagtccgcaatgccagagggctatgtacaagagcgcactattagctttaaggatgatggcacctataagactcgcgcagaggtaaagtttgagggcgatactctcgtaaatcgcattgagctcaagggcattgattttaaggaggatggcaatattctcggccataagctggagtataatttcaattcccataatgtatatattaccgcagataagcaaaagaatggcattaaggcgaattttaagattcgccataatgtggaggatggctccgtacaactcgcagatcattatcaacaaaatactccaattggcgatggcccagtactcctcccagataatcattatctctccactcaatccgtgctctccaaagatccaaatgagaagcgcgatcacatggtactcctggagtttgtaactgcagcaggcattactcatggcatggatgagctctataagctcgagatgctaaagggatgttatgcgtgttgtgagggtttggccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgcagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcact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igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z