LOCUS       BBa_K736003              948 bp    DNA     linear       17-May-2024
DEFINITION  pLAC-regulated TAT-RFP construct
ACCESSION   BBa_K736003
VERSION     BBa_K736003.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    229..948
                     /label=BBa_K736001
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagtg acccgccgcg
      241 cgtttctgaa agcttcctcc gaagacgtta tcgcttcctc cgaagacgtt atcaaagagt
      301 tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag
      361 gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag
      421 gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag
      481 cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt
      541 tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact
      601 cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt
      661 ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt
      721 acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg
      781 gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccgttc agctgccggg
      841 tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact acaccatcgt
      901 tgaacagtac gaacgtgctg aaggtctcac tccaccggtg cttaataa
//