##gff-version 3
##sequence-region BBa_K737004 1 2545
BBa_K737004	.	engineered_region	44	1351	.	+	0	ID=BBa_P0412;Name=BBa_P0412
BBa_K737004	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0412
BBa_K737004	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K737004	.	CDS	62	1189	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0412
BBa_K737004	.	engineered_region	62	1189	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K737004	.	tag	1151	1189	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K737004	.	engineered_tag	1190	1214	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K737004	.	CDS	62	1189	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K737004	.	terminator	1223	1302	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0412
BBa_K737004	.	stem_loop	1234	1277	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K737004	.	engineered_region	1223	1302	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K737004	.	terminator	1311	1351	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0412
BBa_K737004	.	polyA_site	1338	1351	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K737004	.	stem_loop	1318	1337	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K737004	.	stop_codon	1344	1344	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K737004	.	engineered_region	1311	1351	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K737004	.	ribosome_entry_site	1423	1434	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K737004	.	conserved_region	1427	1430	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K737004	.	CDS	1441	1659	.	+	0	ID=BBa_K737016;Name=BBa_K737016
BBa_K737004	.	promoter	1360	1413	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K737004	.	engineered_region	1360	1413	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K737004	.	promoter	1379	1384	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K737004	.	operator	1385	1401	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K737004	.	promoter	1402	1407	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K737004	.	operator	1362	1378	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K737004	.	promoter	1	35	.	+	0	ID=BBa_J23106;Name=BBa_J23106
BBa_K737004	.	engineered_region	1668	2545	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K737004	.	CDS	1689	2408	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K737004	.	CDS	1689	2408	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K737004	.	terminator	2505	2545	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K737004	.	polyA_site	2532	2545	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K737004	.	stem_loop	2512	2531	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K737004	.	stop_codon	2538	2538	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K737004	.	engineered_region	2505	2545	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K737004	.	terminator	2417	2496	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K737004	.	stem_loop	2428	2471	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K737004	.	engineered_region	2417	2496	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K737004	.	ribosome_entry_site	1668	1682	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K737004	.	ribosome_entry_site	1668	1682	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K737004	.	ribosome_entry_site	1675	1679	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K737004	.	engineered_region	1668	1682	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
>BBa_K737004
tttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatggtgaatgtgaaacca
gtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacg
tttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaact
ggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcg
attaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaag
cggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgc
tgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttc
tcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcc
cattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagc
ggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccact
gcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcgg
atatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattt
tcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttg
cccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcat
taatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagag
aaagaggagaaatactagatggccgttgaaaaagtaaactcatcctccagtctggccgaagttatcgatcggatcttag
ataaaggcattgtgattgacgcttgggtacgggtttccctcgttggaatcgagcttctatccatagaagcaagaatcgt
gatcgcttctgttgaaacctatctcaagtacgcagaagccgttggtttaaccgcacaggcggctgttccttcggtctaa
tactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttga
attagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacc
cttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaat
gctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaag
aactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaataga
atcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacaca
atgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaag
cgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctg
tccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctg
ggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttata