LOCUS       BBa_K737005             2361 bp    DNA     linear       21-May-2024
DEFINITION  Inducible GVPC Generator
ACCESSION   BBa_K737005
VERSION     BBa_K737005.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..721
                     /label=BBa_C0040
     stem_loop       766..809
                     /label=stem_loop
     misc_feature    755..834
                     /label=BBa_B0010
     terminator      755..834
                     /label=BBa_B0010
     stem_loop       850..869
                     /label=T7 TE
     misc_feature    876..876
                     /label=stop
     polyA_site      870..883
                     /label=polya
     misc_feature    843..883
                     /label=BBa_B0012
     terminator      843..883
                     /label=BBa_B0012
     misc_feature    44..883
                     /label=BBa_P0440
     misc_feature    892..910
                     /label=TetR 1
     misc_feature    892..945
                     /label=BBa_R0040
     promoter        911..916
                     /label=-35
     misc_feature    917..935
                     /label=TetR 2
     promoter        934..939
                     /label=-10
     promoter        892..945
                     /label=BBa_R0040
     misc_feature    958..961
                     /label=conserved
     RBS             954..965
                     /label=BBa_B0034
     CDS             972..1475
                     /label=BBa_K737017
     RBS             1484..1498
                     /label=RBS-1\Strong
     RBS             1491..1495
                     /label=RBS
     misc_feature    1484..1498
                     /label=BBa_B0030
     RBS             1484..1498
                     /label=BBa_B0030
     CDS             1505..2224
                     /label=GFP protein
     CDS             1505..2224
                     /label=BBa_E0040
     stem_loop       2244..2287
                     /label=stem_loop
     misc_feature    2233..2312
                     /label=BBa_B0010
     terminator      2233..2312
                     /label=BBa_B0010
     stem_loop       2328..2347
                     /label=T7 TE
     misc_feature    2354..2354
                     /label=stop
     polyA_site      2348..2361
                     /label=polya
     misc_feature    2321..2361
                     /label=BBa_B0012
     terminator      2321..2361
                     /label=BBa_B0012
     misc_feature    1484..2361
                     /label=BBa_E0840
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca
      901 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg
      961 agaaatacta gatggcttta aaagacaagt ggcaacagga tcgtatcgga cgccaacagg
     1021 gagttcaaga acggcaacag caagttcaaa ccaccctatc cctctggcaa caagagcgcc
     1081 aaaatcaggc ttctgaattt cgggaagacc tagaatatcg ggtaacggat ctgttagcta
     1141 attatcagaa acagcgccta gaagctaggg aaactttact tgaggactta gctatttttc
     1201 gtcaaaccct atatcgggaa gtcgaagaat atttagggga gttagatatt ctgcaccagc
     1261 aaatggccgc acaattacaa caacaactcc aacagagtcg gacggaaaga aaagacgctg
     1321 ttcagaagtt attcgaggat ttaggggtat ttcgcgccga actacaagac tatcacctca
     1381 aacttcaaca gacagtttgg gggagttccc accgaaaacc gcgaaaagcg attaccccgc
     1441 aacgctctat tccatcgcgt ttatattcct gttaatacta gagattaaag aggagaaata
     1501 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
     1561 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
     1621 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
     1681 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa
     1741 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
     1801 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
     1861 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
     1921 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
     1981 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
     2041 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     2101 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
     2161 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
     2221 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     2281 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     2341 gtgggccttt ctgcgtttat a
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