##gff-version 3
##sequence-region BBa_K737005 1 2361
BBa_K737005	.	CDS	972	1475	.	+	0	ID=BBa_K737017;Name=BBa_K737017
BBa_K737005	.	promoter	892	945	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K737005	.	promoter	911	916	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K737005	.	promoter	934	939	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K737005	.	non_covalent_binding_site	892	910	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K737005	.	engineered_region	892	945	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K737005	.	non_covalent_binding_site	917	935	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K737005	.	engineered_region	44	883	.	+	0	ID=BBa_P0440;Name=BBa_P0440
BBa_K737005	.	terminator	755	834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K737005	.	stem_loop	766	809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K737005	.	engineered_region	755	834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K737005	.	CDS	62	721	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K737005	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K737005	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K737005	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K737005	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K737005	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K737005	.	terminator	843	883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K737005	.	polyA_site	870	883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K737005	.	stem_loop	850	869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K737005	.	engineered_region	843	883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K737005	.	stop_codon	876	876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K737005	.	engineered_region	1484	2361	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K737005	.	terminator	2321	2361	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K737005	.	polyA_site	2348	2361	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K737005	.	stem_loop	2328	2347	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K737005	.	engineered_region	2321	2361	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K737005	.	stop_codon	2354	2354	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K737005	.	CDS	1505	2224	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K737005	.	CDS	1505	2224	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K737005	.	terminator	2233	2312	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K737005	.	stem_loop	2244	2287	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K737005	.	engineered_region	2233	2312	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K737005	.	ribosome_entry_site	1484	1498	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K737005	.	ribosome_entry_site	1484	1498	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K737005	.	ribosome_entry_site	1491	1495	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K737005	.	engineered_region	1484	1498	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K737005	.	promoter	1	35	.	+	0	ID=BBa_J23106;Name=BBa_J23106
BBa_K737005	.	ribosome_entry_site	954	965	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K737005	.	conserved_region	958	961	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K737005
tttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactac
tagagaaagaggagaaatactagatggctttaaaagacaagtggcaacaggatcgtatcggacgccaacagggagttca
agaacggcaacagcaagttcaaaccaccctatccctctggcaacaagagcgccaaaatcaggcttctgaatttcgggaa
gacctagaatatcgggtaacggatctgttagctaattatcagaaacagcgcctagaagctagggaaactttacttgagg
acttagctatttttcgtcaaaccctatatcgggaagtcgaagaatatttaggggagttagatattctgcaccagcaaat
ggccgcacaattacaacaacaactccaacagagtcggacggaaagaaaagacgctgttcagaagttattcgaggattta
ggggtatttcgcgccgaactacaagactatcacctcaaacttcaacagacagtttgggggagttcccaccgaaaaccgc
gaaaagcgattaccccgcaacgctctattccatcgcgtttatattcctgttaatactagagattaaagaggagaaatac
tagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcaca
aattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaa
actacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatg
aaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacggga
actacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaa
agaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaa
aagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaac
aaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaaga
tcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaacta
tacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata