LOCUS       BBa_K737007             1451 bp    DNA     linear       22-Dec-2024
DEFINITION  Constitutive GVPC Generator
ACCESSION   BBa_K737007
VERSION     BBa_K737007.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..565
                     /label=BBa_K737017
     RBS             574..588
                     /label=RBS-1\Strong
     RBS             581..585
                     /label=RBS
     misc_feature    574..588
                     /label=BBa_B0030
     RBS             574..588
                     /label=BBa_B0030
     CDS             595..1314
                     /label=GFP protein
     CDS             595..1314
                     /label=BBa_E0040
     stem_loop       1334..1377
                     /label=stem_loop
     misc_feature    1323..1402
                     /label=BBa_B0010
     terminator      1323..1402
                     /label=BBa_B0010
     stem_loop       1418..1437
                     /label=T7 TE
     misc_feature    1444..1444
                     /label=stop
     polyA_site      1438..1451
                     /label=polya
     misc_feature    1411..1451
                     /label=BBa_B0012
     terminator      1411..1451
                     /label=BBa_B0012
     misc_feature    574..1451
                     /label=BBa_E0840
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatggcttta aaagacaagt ggcaacagga tcgtatcgga cgccaacagg gagttcaaga
      121 acggcaacag caagttcaaa ccaccctatc cctctggcaa caagagcgcc aaaatcaggc
      181 ttctgaattt cgggaagacc tagaatatcg ggtaacggat ctgttagcta attatcagaa
      241 acagcgccta gaagctaggg aaactttact tgaggactta gctatttttc gtcaaaccct
      301 atatcgggaa gtcgaagaat atttagggga gttagatatt ctgcaccagc aaatggccgc
      361 acaattacaa caacaactcc aacagagtcg gacggaaaga aaagacgctg ttcagaagtt
      421 attcgaggat ttaggggtat ttcgcgccga actacaagac tatcacctca aacttcaaca
      481 gacagtttgg gggagttccc accgaaaacc gcgaaaagcg attaccccgc aacgctctat
      541 tccatcgcgt ttatattcct gttaatacta gagattaaag aggagaaata ctagatgcgt
      601 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
      661 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
      721 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
      781 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac
      841 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
      901 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
      961 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
     1021 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
     1081 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat
     1141 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc
     1201 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
     1261 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag
     1321 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1381 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1441 ctgcgtttat a
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