LOCUS       BBa_K737024              976 bp    DNA     linear       16-Feb-2025
DEFINITION  PhoB promotor ligated to GFP
ACCESSION   BBa_K737024
VERSION     BBa_K737024.1  
FEATURES             Location/Qualifiers
     promoter        1..92
                     /label=BBa_K737023
     RBS             101..113
                     /label=RBS-3\Weak
     RBS             107..110
                     /label=RBS
     misc_feature    101..113
                     /label=BBa_B0032
     RBS             101..113
                     /label=BBa_B0032
     CDS             120..839
                     /label=GFP protein
     CDS             120..839
                     /label=BBa_E0040
     stem_loop       859..902
                     /label=stem_loop
     misc_feature    848..927
                     /label=BBa_B0010
     terminator      848..927
                     /label=BBa_B0010
     stem_loop       943..962
                     /label=T7 TE
     misc_feature    969..969
                     /label=stop
     polyA_site      963..976
                     /label=polya
     misc_feature    936..976
                     /label=BBa_B0012
     terminator      936..976
                     /label=BBa_B0012
     misc_feature    101..976
                     /label=BBa_E0240
ORIGIN
        1 acggaaatca ataacctgaa gatatgtgcg acgagctttt cataaatctg tcataaatct
       61 gacgcataat gacgtcgcat taatgatcgc aatactagag tcacacagga aagtactaga
      121 tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
      181 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
      241 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
      301 tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc
      361 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
      421 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta
      481 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
      541 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
      601 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc
      661 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
      721 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc
      781 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaataataat
      841 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      901 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
      961 cctttctgcg tttata
//