BBa_K737034 1 BBa_K737034 galK::GFP generator 2012-09-17T11:00:00Z 2015-05-08T01:13:07Z No Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK???s RBS is a moderate one. */our picture in progressing/* false false _986_ 0 14291 9 It's complicated false Our Reference: [1] Johannes H. Urban and Jo?? rg Vogel, Translational control and target recognition by Escherichia coli small RNAs in vivo, Nucleic Acids Research, 2007, Vol. 35, No. 3 false Peiran Zhang and Chunyan Zhang component2189729 1 BBa_J23106 component2189731 1 BBa_K737066 component2189730 1 BBa_K737033 annotation2189729 1 BBa_J23106 range2189729 1 1 35 annotation2189730 1 BBa_K737033 range2189730 1 44 170 annotation2189731 1 BBa_K737066 range2189731 1 179 895 BBa_K737066 1 BBa_K737066 E0040 without AUG 2012-09-20T11:00:00Z 2015-05-08T01:13:08Z To get more information ,please refer to the part E0040. This part is from E0040,however,it lacks AUG. false false _986_ 0 14291 9 In stock false No false Peiran Zhang BBa_K737033 1 BBa_K737033 galK is the leader sequence of galactokinase (GalK) 2012-09-17T11:00:00Z 2015-05-08T01:13:07Z Reference: [1] Johannes H. Urban and Jo?? rg Vogel, Translational control and target recognition by Escherichia coli small RNAs in vivo, Nucleic Acids Research, 2007, Vol. 35, No. 3 [2] Boris G??rke and J??rg Vogel, Noncoding RNA control of the making and breaking of sugars, GENES & DEVELOPMENT 22:2914???2925 ,2008 Released HQ 2013 galK is the leader sequence of galactokinase (GalK),and Spot42 sRNA controls the synthesis of the galactokinase (GalK) in response to the availability of glucose in the environment,by blocking the recognition of antiSD motif to RBS.The repression is modest(about 2.6 fold),maybe there is no subsequent degradation caused by the major degradosome,RNaesE and RNaseIII. Note:The ClaI site is blocked by GATC methylation due to a mistake.It should be amplified by PCR to get rid of dam methylation before ClaI degestion false false _986_ 0 14291 9 In stock false No false Peiran Zhang BBa_J23106 1 BBa_J23106 constitutive promoter family member 2006-08-13T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K737034_sequence 1 tttacggctagctcagtcctaggtatagtgctagctactagaggtcgacagtcagcgatatccattttcgcgaatccggagtgtaagaaatgagtctgaaagaaaaaacacaatctctgtttgccaacgcatttggctaccctgccactcacaccattcaggcgatcgattactagagcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_J23106_sequence 1 tttacggctagctcagtcctaggtatagtgctagc BBa_K737033_sequence 1 gtcgacagtcagcgatatccattttcgcgaatccggagtgtaagaaatgagtctgaaagaaaaaacacaatctctgtttgccaacgcatttggctaccctgccactcacaccattcaggcgatcgat BBa_K737066_sequence 1 cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z