LOCUS       BBa_K737038             2434 bp    DNA     linear       12-May-2024
DEFINITION  galK::GFP generator ligated to sRNA device  controlled by IPTG
ACCESSION   BBa_K737038
VERSION     BBa_K737038.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1151..1189
                     /label=LVA
     CDS             62..1189
                     /label=lacI-LVA
     misc_feature    62..1189
                     /label=BBa_C0012
     misc_feature    1190..1214
                     /label=Help:Barcodes
     CDS             62..1189
                     /label=BBa_C0012
     stem_loop       1234..1277
                     /label=stem_loop
     misc_feature    1223..1302
                     /label=BBa_B0010
     terminator      1223..1302
                     /label=BBa_B0010
     stem_loop       1318..1337
                     /label=T7 TE
     misc_feature    1344..1344
                     /label=stop
     polyA_site      1338..1351
                     /label=polya
     misc_feature    1311..1351
                     /label=BBa_B0012
     terminator      1311..1351
                     /label=BBa_B0012
     misc_feature    44..1351
                     /label=BBa_P0412
     misc_feature    1362..1378
                     /label=lac O1
     promoter        1379..1384
                     /label=-35
     misc_feature    1385..1401
                     /label=lac O1
     promoter        1402..1407
                     /label=-10
     misc_feature    1360..1413
                     /label=BBa_R0011
     promoter        1360..1413
                     /label=BBa_R0011
     misc_feature    1423..1531
                     /label=BBa_K737058
     promoter        1540..1574
                     /label=BBa_J23106
     RBS             1583..1709
                     /label=BBa_K737033
     CDS             1718..2434
                     /label=BBa_K737066
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
      121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
      181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
      241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
      301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
      361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
      421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
      481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
      541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
      601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
      661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
      721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
      781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
      841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
      901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
      961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg
     1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1321 tcaccttcgg gtgggccttt ctgcgtttat atactagaga attgtgagcg gataacaatt
     1381 gacattgtga gcggataaca agatactgag cacatactag aggtagggta cagaggtaag
     1441 atgttctatc tttcagacct tttacttcac gtaatcggat ttggctgaat attttagccg
     1501 ccccagtcag taatgactgg ggcgtttttt atactagagt ttacggctag ctcagtccta
     1561 ggtatagtgc tagctactag aggtcgacag tcagcgatat ccattttcgc gaatccggag
     1621 tgtaagaaat gagtctgaaa gaaaaaacac aatctctgtt tgccaacgca tttggctacc
     1681 ctgccactca caccattcag gcgatcgatt actagagcgt aaaggagaag aacttttcac
     1741 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
     1801 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
     1861 tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca
     1921 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc
     1981 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
     2041 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
     2101 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa
     2161 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca
     2221 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg
     2281 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
     2341 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
     2401 tacacatggc atggatgaac tatacaaata ataa
//