LOCUS       BBa_K737039             1965 bp    DNA     linear       12-May-2024
DEFINITION  galK::GFP generator ligated to sRNA device controlled by aTc
ACCESSION   BBa_K737039
VERSION     BBa_K737039.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..721
                     /label=BBa_C0040
     stem_loop       766..809
                     /label=stem_loop
     misc_feature    755..834
                     /label=BBa_B0010
     terminator      755..834
                     /label=BBa_B0010
     stem_loop       850..869
                     /label=T7 TE
     misc_feature    876..876
                     /label=stop
     polyA_site      870..883
                     /label=polya
     misc_feature    843..883
                     /label=BBa_B0012
     terminator      843..883
                     /label=BBa_B0012
     misc_feature    44..883
                     /label=BBa_P0440
     misc_feature    892..910
                     /label=TetR 1
     misc_feature    892..945
                     /label=BBa_R0040
     promoter        911..916
                     /label=-35
     misc_feature    917..935
                     /label=TetR 2
     promoter        934..939
                     /label=-10
     promoter        892..945
                     /label=BBa_R0040
     misc_feature    954..1062
                     /label=BBa_K737058
     promoter        1071..1105
                     /label=BBa_J23106
     RBS             1114..1240
                     /label=BBa_K737033
     CDS             1249..1965
                     /label=BBa_K737066
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca
      901 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gaggtagggt
      961 acagaggtaa gatgttctat ctttcagacc ttttacttca cgtaatcgga tttggctgaa
     1021 tattttagcc gccccagtca gtaatgactg gggcgttttt tatactagag tttacggcta
     1081 gctcagtcct aggtatagtg ctagctacta gaggtcgaca gtcagcgata tccattttcg
     1141 cgaatccgga gtgtaagaaa tgagtctgaa agaaaaaaca caatctctgt ttgccaacgc
     1201 atttggctac cctgccactc acaccattca ggcgatcgat tactagagcg taaaggagaa
     1261 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
     1321 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
     1381 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt
     1441 tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag
     1501 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
     1561 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta
     1621 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat
     1681 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc
     1741 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
     1801 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
     1861 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
     1921 gctgctggga ttacacatgg catggatgaa ctatacaaat aataa
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