LOCUS       BBa_K737040             1966 bp    DNA     linear       12-May-2024
DEFINITION  galK::GFP generator ligated to TetR generator and constitutive 
            sRNA device
ACCESSION   BBa_K737040
VERSION     BBa_K737040.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..721
                     /label=BBa_C0040
     stem_loop       766..809
                     /label=stem_loop
     misc_feature    755..834
                     /label=BBa_B0010
     terminator      755..834
                     /label=BBa_B0010
     stem_loop       850..869
                     /label=T7 TE
     misc_feature    876..876
                     /label=stop
     polyA_site      870..883
                     /label=polya
     misc_feature    843..883
                     /label=BBa_B0012
     terminator      843..883
                     /label=BBa_B0012
     misc_feature    44..883
                     /label=BBa_P0440
     misc_feature    894..910
                     /label=lac O1
     promoter        911..916
                     /label=-35
     misc_feature    917..933
                     /label=lac O1
     promoter        934..939
                     /label=-10
     misc_feature    892..945
                     /label=BBa_R0011
     promoter        892..945
                     /label=BBa_R0011
     misc_feature    955..1063
                     /label=BBa_K737058
     promoter        1072..1106
                     /label=BBa_J23106
     RBS             1115..1241
                     /label=BBa_K737033
     CDS             1250..1966
                     /label=BBa_K737066
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gaattgtgag
      901 cggataacaa ttgacattgt gagcggataa caagatactg agcacatact agaggtaggg
      961 tacagaggta agatgttcta tctttcagac cttttacttc acgtaatcgg atttggctga
     1021 atattttagc cgccccagtc agtaatgact ggggcgtttt ttatactaga gtttacggct
     1081 agctcagtcc taggtatagt gctagctact agaggtcgac agtcagcgat atccattttc
     1141 gcgaatccgg agtgtaagaa atgagtctga aagaaaaaac acaatctctg tttgccaacg
     1201 catttggcta ccctgccact cacaccattc aggcgatcga ttactagagc gtaaaggaga
     1261 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
     1321 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
     1381 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
     1441 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
     1501 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
     1561 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
     1621 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
     1681 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
     1741 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
     1801 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
     1861 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
     1921 agctgctggg attacacatg gcatggatga actatacaaa taataa
//