LOCUS BBa_K737040 1966 bp DNA linear 22-Dec-2024 DEFINITION galK::GFP generator ligated to TetR generator and constitutive sRNA device ACCESSION BBa_K737040 VERSION BBa_K737040.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23106 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 65..681 /label=tetR misc_feature 682..715 /label=SsrA misc_feature 722..746 /label=Help:Barcodes CDS 62..721 /label=BBa_C0040 stem_loop 766..809 /label=stem_loop misc_feature 755..834 /label=BBa_B0010 terminator 755..834 /label=BBa_B0010 stem_loop 850..869 /label=T7 TE misc_feature 876..876 /label=stop polyA_site 870..883 /label=polya misc_feature 843..883 /label=BBa_B0012 terminator 843..883 /label=BBa_B0012 misc_feature 44..883 /label=BBa_P0440 misc_feature 894..910 /label=lac O1 promoter 911..916 /label=-35 misc_feature 917..933 /label=lac O1 promoter 934..939 /label=-10 misc_feature 892..945 /label=BBa_R0011 promoter 892..945 /label=BBa_R0011 misc_feature 955..1063 /label=BBa_K737058 promoter 1072..1106 /label=BBa_J23106 RBS 1115..1241 /label=BBa_K737033 CDS 1250..1966 /label=BBa_K737066 ORIGIN 1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta 61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gaattgtgag 901 cggataacaa ttgacattgt gagcggataa caagatactg agcacatact agaggtaggg 961 tacagaggta agatgttcta tctttcagac cttttacttc acgtaatcgg atttggctga 1021 atattttagc cgccccagtc agtaatgact ggggcgtttt ttatactaga gtttacggct 1081 agctcagtcc taggtatagt gctagctact agaggtcgac agtcagcgat atccattttc 1141 gcgaatccgg agtgtaagaa atgagtctga aagaaaaaac acaatctctg tttgccaacg 1201 catttggcta ccctgccact cacaccattc aggcgatcga ttactagagc gtaaaggaga 1261 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca 1321 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa 1381 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg 1441 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa 1501 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa 1561 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt 1621 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta 1681 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt 1741 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa 1801 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc 1861 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac 1921 agctgctggg attacacatg gcatggatga actatacaaa taataa //