LOCUS       BBa_K737041             2433 bp    DNA     linear       13-May-2024
DEFINITION  galK::GFP generator ligated to LacI generator and constitutive 
            sRNA device
ACCESSION   BBa_K737041
VERSION     BBa_K737041.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1151..1189
                     /label=LVA
     CDS             62..1189
                     /label=lacI-LVA
     misc_feature    62..1189
                     /label=BBa_C0012
     misc_feature    1190..1214
                     /label=Help:Barcodes
     CDS             62..1189
                     /label=BBa_C0012
     stem_loop       1234..1277
                     /label=stem_loop
     misc_feature    1223..1302
                     /label=BBa_B0010
     terminator      1223..1302
                     /label=BBa_B0010
     stem_loop       1318..1337
                     /label=T7 TE
     misc_feature    1344..1344
                     /label=stop
     polyA_site      1338..1351
                     /label=polya
     misc_feature    1311..1351
                     /label=BBa_B0012
     terminator      1311..1351
                     /label=BBa_B0012
     misc_feature    44..1351
                     /label=BBa_P0412
     misc_feature    1360..1378
                     /label=TetR 1
     misc_feature    1360..1413
                     /label=BBa_R0040
     promoter        1379..1384
                     /label=-35
     misc_feature    1385..1403
                     /label=TetR 2
     promoter        1402..1407
                     /label=-10
     promoter        1360..1413
                     /label=BBa_R0040
     misc_feature    1422..1530
                     /label=BBa_K737058
     promoter        1539..1573
                     /label=BBa_J23106
     RBS             1582..1708
                     /label=BBa_K737033
     CDS             1717..2433
                     /label=BBa_K737066
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
      121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
      181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
      241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
      301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
      361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
      421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
      481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
      541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
      601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
      661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
      721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
      781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
      841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
      901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
      961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg
     1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1321 tcaccttcgg gtgggccttt ctgcgtttat atactagagt ccctatcagt gatagagatt
     1381 gacatcccta tcagtgatag agatactgag cactactaga ggtagggtac agaggtaaga
     1441 tgttctatct ttcagacctt ttacttcacg taatcggatt tggctgaata ttttagccgc
     1501 cccagtcagt aatgactggg gcgtttttta tactagagtt tacggctagc tcagtcctag
     1561 gtatagtgct agctactaga ggtcgacagt cagcgatatc cattttcgcg aatccggagt
     1621 gtaagaaatg agtctgaaag aaaaaacaca atctctgttt gccaacgcat ttggctaccc
     1681 tgccactcac accattcagg cgatcgatta ctagagcgta aaggagaaga acttttcact
     1741 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
     1801 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
     1861 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa
     1921 tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc
     1981 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt
     2041 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat
     2101 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat
     2161 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac
     2221 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc
     2281 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa
     2341 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt
     2401 acacatggca tggatgaact atacaaataa taa
//