LOCUS BBa_K737045 3657 bp DNA linear 22-Dec-2024 DEFINITION YtfJ_Comparator ACCESSION BBa_K737045 VERSION BBa_K737045.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23106 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 65..681 /label=tetR misc_feature 682..715 /label=SsrA misc_feature 722..746 /label=Help:Barcodes CDS 62..721 /label=BBa_C0040 stem_loop 766..809 /label=stem_loop misc_feature 755..834 /label=BBa_B0010 terminator 755..834 /label=BBa_B0010 stem_loop 850..869 /label=T7 TE misc_feature 876..876 /label=stop polyA_site 870..883 /label=polya misc_feature 843..883 /label=BBa_B0012 terminator 843..883 /label=BBa_B0012 misc_feature 44..883 /label=BBa_P0440 misc_feature 894..910 /label=lac O1 promoter 911..916 /label=-35 misc_feature 917..933 /label=lac O1 promoter 934..939 /label=-10 misc_feature 892..945 /label=BBa_R0011 promoter 892..945 /label=BBa_R0011 misc_feature 955..1216 /label=BBa_K737049 promoter 1225..1259 /label=BBa_J23106 misc_feature 1272..1275 /label=conserved RBS 1268..1279 /label=BBa_B0034 misc_feature 2375..2413 /label=LVA CDS 1286..2413 /label=lacI-LVA misc_feature 1286..2413 /label=BBa_C0012 misc_feature 2414..2438 /label=Help:Barcodes CDS 1286..2413 /label=BBa_C0012 stem_loop 2458..2501 /label=stem_loop misc_feature 2447..2526 /label=BBa_B0010 terminator 2447..2526 /label=BBa_B0010 stem_loop 2542..2561 /label=T7 TE misc_feature 2568..2568 /label=stop polyA_site 2562..2575 /label=polya misc_feature 2535..2575 /label=BBa_B0012 terminator 2535..2575 /label=BBa_B0012 misc_feature 1268..2575 /label=BBa_P0412 misc_feature 2584..2602 /label=TetR 1 misc_feature 2584..2637 /label=BBa_R0040 promoter 2603..2608 /label=-35 misc_feature 2609..2627 /label=TetR 2 promoter 2626..2631 /label=-10 promoter 2584..2637 /label=BBa_R0040 misc_feature 2646..2754 /label=BBa_K737058 promoter 2763..2797 /label=BBa_J23106 RBS 2806..2932 /label=BBa_K737033 CDS 2941..3657 /label=BBa_K737066 ORIGIN 1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta 61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gaattgtgag 901 cggataacaa ttgacattgt gagcggataa caagatactg agcacatact agagcgcggt 961 cttcagtata agcattcatt tttcatatga tttgtgcgct tgaccgcaaa ctggcatcac 1021 acttgcggga aattcgataa atagcacata tgattaaaac tcagacccaa gtggtcggat 1081 cacctgcata tcataagaag gaaacaccat gaccctacgc aagattctgg cactcacctg 1141 cctgctgttg ccgatgatgg cttccgcaca tcagttcgaa accggtcagc gagtgccgcc 1201 gattggcatt accgattact agagtttacg gctagctcag tcctaggtat agtgctagct 1261 actagagaaa gaggagaaat actagatggt gaatgtgaaa ccagtaacgt tatacgatgt 1321 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca 1381 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc 1441 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc 1501 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca 1561 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc 1621 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga 1681 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga 1741 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact 1801 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt 1861 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca 1921 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac 1981 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat 2041 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc 2101 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat 2161 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca 2221 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac 2281 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 2341 ggcacgacag gtttcccgac tggaaagcgg gcaggctgca aacgacgaaa actacgcttt 2401 agtagcttaa taactctgat agtgctagtg tagatctcta ctagagccag gcatcaaata 2461 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 2521 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 2581 gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac tgagcactac 2641 tagaggtagg gtacagaggt aagatgttct atctttcaga ccttttactt cacgtaatcg 2701 gatttggctg aatattttag ccgccccagt cagtaatgac tggggcgttt tttatactag 2761 agtttacggc tagctcagtc ctaggtatag tgctagctac tagaggtcga cagtcagcga 2821 tatccatttt cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct 2881 gtttgccaac gcatttggct accctgccac tcacaccatt caggcgatcg attactagag 2941 cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat 3001 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa 3061 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc 3121 actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat 3181 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa 3241 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat 3301 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg 3361 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc 3421 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat 3481 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg 3541 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt 3601 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa ataataa //