##gff-version 3
##sequence-region BBa_K737045 1 3657
BBa_K737045	.	engineered_region	1268	2575	.	+	0	ID=BBa_P0412;Name=BBa_P0412
BBa_K737045	.	ribosome_entry_site	1268	1279	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0412
BBa_K737045	.	conserved_region	1272	1275	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K737045	.	CDS	1286	2413	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0412
BBa_K737045	.	engineered_region	1286	2413	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K737045	.	engineered_tag	2414	2438	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K737045	.	tag	2375	2413	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K737045	.	CDS	1286	2413	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K737045	.	terminator	2535	2575	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0412
BBa_K737045	.	stem_loop	2542	2561	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K737045	.	engineered_region	2535	2575	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K737045	.	polyA_site	2562	2575	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K737045	.	stop_codon	2568	2568	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K737045	.	terminator	2447	2526	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0412
BBa_K737045	.	engineered_region	2447	2526	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K737045	.	stem_loop	2458	2501	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K737045	.	mature_transcript_region	2646	2754	.	+	0	ID=BBa_K737058;Name=BBa_K737058
BBa_K737045	.	promoter	892	945	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K737045	.	operator	917	933	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K737045	.	engineered_region	892	945	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K737045	.	promoter	934	939	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K737045	.	operator	894	910	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K737045	.	promoter	911	916	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K737045	.	CDS	2941	3657	.	+	0	ID=BBa_K737066;Name=BBa_K737066
BBa_K737045	.	promoter	1225	1259	.	+	0	ID=BBa_J23106;Name=BBa_J23106
BBa_K737045	.	engineered_region	44	883	.	+	0	ID=BBa_P0440;Name=BBa_P0440
BBa_K737045	.	terminator	843	883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K737045	.	stem_loop	850	869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K737045	.	engineered_region	843	883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K737045	.	polyA_site	870	883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K737045	.	stop_codon	876	876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K737045	.	terminator	755	834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K737045	.	engineered_region	755	834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K737045	.	stem_loop	766	809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K737045	.	CDS	62	721	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K737045	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K737045	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K737045	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K737045	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K737045	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K737045	.	promoter	1	35	.	+	0	ID=BBa_J23106;Name=BBa_J23106
BBa_K737045	.	ribosome_entry_site	2806	2932	.	+	0	ID=BBa_K737033;Name=BBa_K737033
BBa_K737045	.	promoter	2763	2797	.	+	0	ID=BBa_J23106;Name=BBa_J23106
BBa_K737045	.	mature_transcript_region	955	1216	.	+	0	ID=BBa_K737049;Name=BBa_K737049
BBa_K737045	.	promoter	2584	2637	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K737045	.	promoter	2626	2631	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K737045	.	promoter	2603	2608	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K737045	.	engineered_region	2584	2637	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K737045	.	non_covalent_binding_site	2609	2627	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K737045	.	non_covalent_binding_site	2584	2602	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_K737045
tttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacata
ctagagcgcggtcttcagtataagcattcatttttcatatgatttgtgcgcttgaccgcaaactggcatcacacttgcg
ggaaattcgataaatagcacatatgattaaaactcagacccaagtggtcggatcacctgcatatcataagaaggaaaca
ccatgaccctacgcaagattctggcactcacctgcctgctgttgccgatgatggcttccgcacatcagttcgaaaccgg
tcagcgagtgccgccgattggcattaccgattactagagtttacggctagctcagtcctaggtatagtgctagctacta
gagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctctt
atcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggc
ggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctcc
agtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtgg
tgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggct
gatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttctt
gatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctgg
tcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctg
gcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaa
caaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaa
tgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctc
atgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaa
ctctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgccca
atacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgg
gcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgac
atccctatcagtgatagagatactgagcactactagaggtagggtacagaggtaagatgttctatctttcagacctttt
acttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatgactggggcgttttttatactagagttt
acggctagctcagtcctaggtatagtgctagctactagaggtcgacagtcagcgatatccattttcgcgaatccggagt
gtaagaaatgagtctgaaagaaaaaacacaatctctgtttgccaacgcatttggctaccctgccactcacaccattcag
gcgatcgattactagagcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgt
taatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgc
actactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacc
cagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaa
agatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggt
attgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatgg
cagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcaga
ccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgcc
ctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggca
tggatgaactatacaaataataa