LOCUS BBa_K737046 3801 bp DNA linear 22-Nov-2024 DEFINITION SrlA_Comparator ACCESSION BBa_K737046 VERSION BBa_K737046.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23106 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 65..681 /label=tetR misc_feature 682..715 /label=SsrA misc_feature 722..746 /label=Help:Barcodes CDS 62..721 /label=BBa_C0040 stem_loop 766..809 /label=stem_loop misc_feature 755..834 /label=BBa_B0010 terminator 755..834 /label=BBa_B0010 stem_loop 850..869 /label=T7 TE misc_feature 876..876 /label=stop polyA_site 870..883 /label=polya misc_feature 843..883 /label=BBa_B0012 terminator 843..883 /label=BBa_B0012 misc_feature 44..883 /label=BBa_P0440 misc_feature 894..910 /label=lac O1 promoter 911..916 /label=-35 misc_feature 917..933 /label=lac O1 promoter 934..939 /label=-10 misc_feature 892..945 /label=BBa_R0011 promoter 892..945 /label=BBa_R0011 misc_feature 955..1360 /label=BBa_K737050 promoter 1369..1403 /label=BBa_J23106 misc_feature 1416..1419 /label=conserved RBS 1412..1423 /label=BBa_B0034 misc_feature 2519..2557 /label=LVA CDS 1430..2557 /label=lacI-LVA misc_feature 1430..2557 /label=BBa_C0012 misc_feature 2558..2582 /label=Help:Barcodes CDS 1430..2557 /label=BBa_C0012 stem_loop 2602..2645 /label=stem_loop misc_feature 2591..2670 /label=BBa_B0010 terminator 2591..2670 /label=BBa_B0010 stem_loop 2686..2705 /label=T7 TE misc_feature 2712..2712 /label=stop polyA_site 2706..2719 /label=polya misc_feature 2679..2719 /label=BBa_B0012 terminator 2679..2719 /label=BBa_B0012 misc_feature 1412..2719 /label=BBa_P0412 misc_feature 2728..2746 /label=TetR 1 misc_feature 2728..2781 /label=BBa_R0040 promoter 2747..2752 /label=-35 misc_feature 2753..2771 /label=TetR 2 promoter 2770..2775 /label=-10 promoter 2728..2781 /label=BBa_R0040 misc_feature 2790..2898 /label=BBa_K737058 promoter 2907..2941 /label=BBa_J23106 RBS 2950..3076 /label=BBa_K737033 CDS 3085..3801 /label=BBa_K737066 ORIGIN 1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta 61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gaattgtgag 901 cggataacaa ttgacattgt gagcggataa caagatactg agcacatact agagttgccg 961 tttggtaata aaacaataaa tcctgaagga gagaacaatg atagaaacca ttactcatgg 1021 tgcagagtgg tttatcgggc tgttccaaaa gggcggagag gtgtttaccg ggatggtgac 1081 cggcattctt ccgctgttga ttagcctgct ggttatcatg aacgcactga ttaattttat 1141 cggtcagcat cgtattgaac gttttgctca acgttgcgcc ggtaaccctg tttcccgtta 1201 cctactgtta ccgtgcattg gcacgtttgt cttttgcaat ccgatgaccc taagcctggg 1261 tcgctttatg ccggaaaagt acaaacccag ctactacgcg gcggcctctt atagctgcca 1321 ctcaatgaat ggcctcttcc cccatatcaa ccctggcgaa tactagagtt tacggctagc 1381 tcagtcctag gtatagtgct agctactaga gaaagaggag aaatactaga tggtgaatgt 1441 gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc 1501 ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc 1561 gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc 1621 gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc 1681 ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg 1741 aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg 1801 gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac 1861 taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt 1921 ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca 1981 aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg 2041 gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag 2101 tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc 2161 gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg 2221 gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg 2281 ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt 2341 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt 2401 ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc 2461 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcaggc 2521 tgcaaacgac gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc 2581 tctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 2641 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 2701 gggcctttct gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc 2761 agtgatagag atactgagca ctactagagg tagggtacag aggtaagatg ttctatcttt 2821 cagacctttt acttcacgta atcggatttg gctgaatatt ttagccgccc cagtcagtaa 2881 tgactggggc gttttttata ctagagttta cggctagctc agtcctaggt atagtgctag 2941 ctactagagg tcgacagtca gcgatatcca ttttcgcgaa tccggagtgt aagaaatgag 3001 tctgaaagaa aaaacacaat ctctgtttgc caacgcattt ggctaccctg ccactcacac 3061 cattcaggcg atcgattact agagcgtaaa ggagaagaac ttttcactgg agttgtccca 3121 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt 3181 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta 3241 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga 3301 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta 3361 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag 3421 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat 3481 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg 3541 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat 3601 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc 3661 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa 3721 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg 3781 gatgaactat acaaataata a //