LOCUS BBa_K737047 3522 bp DNA linear 29-Nov-2024 DEFINITION GalK_Comparator ACCESSION BBa_K737047 VERSION BBa_K737047.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23106 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 65..681 /label=tetR misc_feature 682..715 /label=SsrA misc_feature 722..746 /label=Help:Barcodes CDS 62..721 /label=BBa_C0040 stem_loop 766..809 /label=stem_loop misc_feature 755..834 /label=BBa_B0010 terminator 755..834 /label=BBa_B0010 stem_loop 850..869 /label=T7 TE misc_feature 876..876 /label=stop polyA_site 870..883 /label=polya misc_feature 843..883 /label=BBa_B0012 terminator 843..883 /label=BBa_B0012 misc_feature 44..883 /label=BBa_P0440 misc_feature 894..910 /label=lac O1 promoter 911..916 /label=-35 misc_feature 917..933 /label=lac O1 promoter 934..939 /label=-10 misc_feature 892..945 /label=BBa_R0011 promoter 892..945 /label=BBa_R0011 RBS 955..1081 /label=BBa_K737033 promoter 1090..1124 /label=BBa_J23106 misc_feature 1137..1140 /label=conserved RBS 1133..1144 /label=BBa_B0034 misc_feature 2240..2278 /label=LVA CDS 1151..2278 /label=lacI-LVA misc_feature 1151..2278 /label=BBa_C0012 misc_feature 2279..2303 /label=Help:Barcodes CDS 1151..2278 /label=BBa_C0012 stem_loop 2323..2366 /label=stem_loop misc_feature 2312..2391 /label=BBa_B0010 terminator 2312..2391 /label=BBa_B0010 stem_loop 2407..2426 /label=T7 TE misc_feature 2433..2433 /label=stop polyA_site 2427..2440 /label=polya misc_feature 2400..2440 /label=BBa_B0012 terminator 2400..2440 /label=BBa_B0012 misc_feature 1133..2440 /label=BBa_P0412 misc_feature 2449..2467 /label=TetR 1 misc_feature 2449..2502 /label=BBa_R0040 promoter 2468..2473 /label=-35 misc_feature 2474..2492 /label=TetR 2 promoter 2491..2496 /label=-10 promoter 2449..2502 /label=BBa_R0040 misc_feature 2511..2619 /label=BBa_K737058 promoter 2628..2662 /label=BBa_J23106 RBS 2671..2797 /label=BBa_K737033 CDS 2806..3522 /label=BBa_K737066 ORIGIN 1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta 61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gaattgtgag 901 cggataacaa ttgacattgt gagcggataa caagatactg agcacatact agaggtcgac 961 agtcagcgat atccattttc gcgaatccgg agtgtaagaa atgagtctga aagaaaaaac 1021 acaatctctg tttgccaacg catttggcta ccctgccact cacaccattc aggcgatcga 1081 ttactagagt ttacggctag ctcagtccta ggtatagtgc tagctactag agaaagagga 1141 gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg 1201 tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac 1261 gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca 1321 acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca 1381 cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt 1441 ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct 1501 tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat 1561 tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac 1621 acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct 1681 ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc 1741 gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc 1801 ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa 1861 tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat 1921 gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga 1981 cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg 2041 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa 2101 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac 2161 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 2221 ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag cttaataact 2281 ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg aaaggctcag 2341 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 2401 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtc cctatcagtg 2461 atagagattg acatccctat cagtgataga gatactgagc actactagag gtagggtaca 2521 gaggtaagat gttctatctt tcagaccttt tacttcacgt aatcggattt ggctgaatat 2581 tttagccgcc ccagtcagta atgactgggg cgttttttat actagagttt acggctagct 2641 cagtcctagg tatagtgcta gctactagag gtcgacagtc agcgatatcc attttcgcga 2701 atccggagtg taagaaatga gtctgaaaga aaaaacacaa tctctgtttg ccaacgcatt 2761 tggctaccct gccactcaca ccattcaggc gatcgattac tagagcgtaa aggagaagaa 2821 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa 2881 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt 2941 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat 3001 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt 3061 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac 3121 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa 3181 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac 3241 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa 3301 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact 3361 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc 3421 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct 3481 gctgggatta cacatggcat ggatgaacta tacaaataat aa //