##gff-version 3
##sequence-region BBa_K737047 1 3522
BBa_K737047	.	promoter	1	35	.	+	0	ID=BBa_J23106;Name=BBa_J23106
BBa_K737047	.	promoter	892	945	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K737047	.	promoter	934	939	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K737047	.	promoter	911	916	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K737047	.	operator	917	933	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K737047	.	engineered_region	892	945	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K737047	.	operator	894	910	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K737047	.	promoter	1090	1124	.	+	0	ID=BBa_J23106;Name=BBa_J23106
BBa_K737047	.	ribosome_entry_site	955	1081	.	+	0	ID=BBa_K737033;Name=BBa_K737033
BBa_K737047	.	mature_transcript_region	2511	2619	.	+	0	ID=BBa_K737058;Name=BBa_K737058
BBa_K737047	.	ribosome_entry_site	2671	2797	.	+	0	ID=BBa_K737033;Name=BBa_K737033
BBa_K737047	.	CDS	2806	3522	.	+	0	ID=BBa_K737066;Name=BBa_K737066
BBa_K737047	.	engineered_region	44	883	.	+	0	ID=BBa_P0440;Name=BBa_P0440
BBa_K737047	.	terminator	755	834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K737047	.	engineered_region	755	834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K737047	.	stem_loop	766	809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K737047	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K737047	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K737047	.	terminator	843	883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K737047	.	engineered_region	843	883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K737047	.	stop_codon	876	876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K737047	.	stem_loop	850	869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K737047	.	polyA_site	870	883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K737047	.	CDS	62	721	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K737047	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K737047	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K737047	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K737047	.	engineered_region	1133	2440	.	+	0	ID=BBa_P0412;Name=BBa_P0412
BBa_K737047	.	terminator	2400	2440	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0412
BBa_K737047	.	engineered_region	2400	2440	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K737047	.	stop_codon	2433	2433	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K737047	.	stem_loop	2407	2426	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K737047	.	polyA_site	2427	2440	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K737047	.	CDS	1151	2278	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0412
BBa_K737047	.	engineered_tag	2279	2303	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K737047	.	engineered_region	1151	2278	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K737047	.	CDS	1151	2278	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K737047	.	tag	2240	2278	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K737047	.	terminator	2312	2391	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0412
BBa_K737047	.	engineered_region	2312	2391	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K737047	.	stem_loop	2323	2366	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K737047	.	ribosome_entry_site	1133	1144	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0412
BBa_K737047	.	conserved_region	1137	1140	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K737047	.	promoter	2449	2502	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K737047	.	promoter	2468	2473	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K737047	.	non_covalent_binding_site	2474	2492	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K737047	.	engineered_region	2449	2502	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K737047	.	promoter	2491	2496	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K737047	.	non_covalent_binding_site	2449	2467	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K737047	.	promoter	2628	2662	.	+	0	ID=BBa_J23106;Name=BBa_J23106
>BBa_K737047
tttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacata
ctagaggtcgacagtcagcgatatccattttcgcgaatccggagtgtaagaaatgagtctgaaagaaaaaacacaatct
ctgtttgccaacgcatttggctaccctgccactcacaccattcaggcgatcgattactagagtttacggctagctcagt
cctaggtatagtgctagctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgt
cgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgg
gaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgt
tgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccga
tcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatctt
ctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgca
ctaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtac
gcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcg
gcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgact
ggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaa
cgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtggga
tacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaa
ccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaa
aagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacga
caggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgct
agtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagt
ccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagaggtagggtacagaggtaag
atgttctatctttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatgactg
gggcgttttttatactagagtttacggctagctcagtcctaggtatagtgctagctactagaggtcgacagtcagcgat
atccattttcgcgaatccggagtgtaagaaatgagtctgaaagaaaaaacacaatctctgtttgccaacgcatttggct
accctgccactcacaccattcaggcgatcgattactagagcgtaaaggagaagaacttttcactggagttgtcccaatt
cttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaa
aacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatgg
tgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgta
caggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttg
ttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataa
ctcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaa
gatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaacc
attacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaac
agctgctgggattacacatggcatggatgaactatacaaataataa