LOCUS       BBa_K737053             1830 bp    DNA     linear       10-May-2024
DEFINITION  Plac-GFP Generator ligated to TetR Generator
ACCESSION   BBa_K737053
VERSION     BBa_K737053.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..721
                     /label=BBa_C0040
     stem_loop       766..809
                     /label=stem_loop
     misc_feature    755..834
                     /label=BBa_B0010
     terminator      755..834
                     /label=BBa_B0010
     stem_loop       850..869
                     /label=T7 TE
     misc_feature    876..876
                     /label=stop
     polyA_site      870..883
                     /label=polya
     misc_feature    843..883
                     /label=BBa_B0012
     terminator      843..883
                     /label=BBa_B0012
     misc_feature    44..883
                     /label=BBa_P0440
     misc_feature    894..910
                     /label=lac O1
     promoter        911..916
                     /label=-35
     misc_feature    917..933
                     /label=lac O1
     promoter        934..939
                     /label=-10
     misc_feature    892..945
                     /label=BBa_R0011
     promoter        892..945
                     /label=BBa_R0011
     RBS             955..967
                     /label=RBS-3\Weak
     RBS             961..964
                     /label=RBS
     misc_feature    955..967
                     /label=BBa_B0032
     RBS             955..967
                     /label=BBa_B0032
     CDS             974..1693
                     /label=GFP protein
     CDS             974..1693
                     /label=BBa_E0040
     stem_loop       1713..1756
                     /label=stem_loop
     misc_feature    1702..1781
                     /label=BBa_B0010
     terminator      1702..1781
                     /label=BBa_B0010
     stem_loop       1797..1816
                     /label=T7 TE
     misc_feature    1823..1823
                     /label=stop
     polyA_site      1817..1830
                     /label=polya
     misc_feature    1790..1830
                     /label=BBa_B0012
     terminator      1790..1830
                     /label=BBa_B0012
     misc_feature    955..1830
                     /label=BBa_E0240
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gaattgtgag
      901 cggataacaa ttgacattgt gagcggataa caagatactg agcacatact agagtcacac
      961 aggaaagtac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt
     1021 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
     1081 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
     1141 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
     1201 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
     1261 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
     1321 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
     1381 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
     1441 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
     1501 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
     1561 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
     1621 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
     1681 atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1741 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1801 caccttcggg tgggcctttc tgcgtttata
//