LOCUS       BBa_K737054             2297 bp    DNA     linear       22-Dec-2024
DEFINITION  Ptet-GFP Generator ligated to LacI Generator
ACCESSION   BBa_K737054
VERSION     BBa_K737054.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1151..1189
                     /label=LVA
     CDS             62..1189
                     /label=lacI-LVA
     misc_feature    62..1189
                     /label=BBa_C0012
     misc_feature    1190..1214
                     /label=Help:Barcodes
     CDS             62..1189
                     /label=BBa_C0012
     stem_loop       1234..1277
                     /label=stem_loop
     misc_feature    1223..1302
                     /label=BBa_B0010
     terminator      1223..1302
                     /label=BBa_B0010
     stem_loop       1318..1337
                     /label=T7 TE
     misc_feature    1344..1344
                     /label=stop
     polyA_site      1338..1351
                     /label=polya
     misc_feature    1311..1351
                     /label=BBa_B0012
     terminator      1311..1351
                     /label=BBa_B0012
     misc_feature    44..1351
                     /label=BBa_P0412
     misc_feature    1360..1378
                     /label=TetR 1
     misc_feature    1360..1413
                     /label=BBa_R0040
     promoter        1379..1384
                     /label=-35
     misc_feature    1385..1403
                     /label=TetR 2
     promoter        1402..1407
                     /label=-10
     promoter        1360..1413
                     /label=BBa_R0040
     RBS             1422..1434
                     /label=RBS-3\Weak
     RBS             1428..1431
                     /label=RBS
     misc_feature    1422..1434
                     /label=BBa_B0032
     RBS             1422..1434
                     /label=BBa_B0032
     CDS             1441..2160
                     /label=GFP protein
     CDS             1441..2160
                     /label=BBa_E0040
     stem_loop       2180..2223
                     /label=stem_loop
     misc_feature    2169..2248
                     /label=BBa_B0010
     terminator      2169..2248
                     /label=BBa_B0010
     stem_loop       2264..2283
                     /label=T7 TE
     misc_feature    2290..2290
                     /label=stop
     polyA_site      2284..2297
                     /label=polya
     misc_feature    2257..2297
                     /label=BBa_B0012
     terminator      2257..2297
                     /label=BBa_B0012
     misc_feature    1422..2297
                     /label=BBa_E0240
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
      121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
      181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
      241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
      301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
      361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
      421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
      481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
      541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
      601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
      661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
      721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
      781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
      841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
      901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
      961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg
     1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1321 tcaccttcgg gtgggccttt ctgcgtttat atactagagt ccctatcagt gatagagatt
     1381 gacatcccta tcagtgatag agatactgag cactactaga gtcacacagg aaagtactag
     1441 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
     1501 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
     1561 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
     1621 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
     1681 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
     1741 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
     1801 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
     1861 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
     1921 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
     1981 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     2041 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     2101 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa
     2161 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     2221 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     2281 gcctttctgc gtttata
//