##gff-version 3
##sequence-region BBa_K737054 1 2297
BBa_K737054	.	engineered_region	1422	2297	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_K737054	.	terminator	2169	2248	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K737054	.	engineered_region	2169	2248	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K737054	.	stem_loop	2180	2223	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K737054	.	CDS	1441	2160	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K737054	.	CDS	1441	2160	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K737054	.	ribosome_entry_site	1422	1434	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K737054	.	ribosome_entry_site	1428	1431	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K737054	.	ribosome_entry_site	1422	1434	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K737054	.	engineered_region	1422	1434	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K737054	.	terminator	2257	2297	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K737054	.	engineered_region	2257	2297	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K737054	.	stop_codon	2290	2290	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K737054	.	stem_loop	2264	2283	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K737054	.	polyA_site	2284	2297	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K737054	.	promoter	1	35	.	+	0	ID=BBa_J23106;Name=BBa_J23106
BBa_K737054	.	engineered_region	44	1351	.	+	0	ID=BBa_P0412;Name=BBa_P0412
BBa_K737054	.	terminator	1311	1351	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0412
BBa_K737054	.	engineered_region	1311	1351	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K737054	.	stop_codon	1344	1344	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K737054	.	stem_loop	1318	1337	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K737054	.	polyA_site	1338	1351	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K737054	.	CDS	62	1189	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0412
BBa_K737054	.	engineered_tag	1190	1214	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K737054	.	engineered_region	62	1189	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K737054	.	CDS	62	1189	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K737054	.	tag	1151	1189	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K737054	.	terminator	1223	1302	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0412
BBa_K737054	.	engineered_region	1223	1302	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K737054	.	stem_loop	1234	1277	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K737054	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0412
BBa_K737054	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K737054	.	promoter	1360	1413	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K737054	.	promoter	1379	1384	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K737054	.	non_covalent_binding_site	1385	1403	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K737054	.	engineered_region	1360	1413	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K737054	.	promoter	1402	1407	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K737054	.	non_covalent_binding_site	1360	1378	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_K737054
tttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatggtgaatgtgaaacca
gtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacg
tttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaact
ggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcg
attaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaag
cggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgc
tgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttc
tcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcc
cattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagc
ggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccact
gcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcgg
atatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattt
tcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttg
cccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcat
taatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagt
cacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtg
atgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttat
ttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgaga
tacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttt
tcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaa
aggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatc
atggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactag
cagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatc
tgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacat
ggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttata