LOCUS BBa_K737055 2962 bp DNA linear 23-Dec-2024 DEFINITION NanC_s1s2_Gnt ACCESSION BBa_K737055 VERSION BBa_K737055.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23106 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 65..681 /label=tetR misc_feature 682..715 /label=SsrA misc_feature 722..746 /label=Help:Barcodes CDS 62..721 /label=BBa_C0040 stem_loop 766..809 /label=stem_loop misc_feature 755..834 /label=BBa_B0010 terminator 755..834 /label=BBa_B0010 stem_loop 850..869 /label=T7 TE misc_feature 876..876 /label=stop polyA_site 870..883 /label=polya misc_feature 843..883 /label=BBa_B0012 terminator 843..883 /label=BBa_B0012 misc_feature 44..883 /label=BBa_P0440 misc_feature 894..910 /label=lac O1 promoter 911..916 /label=-35 misc_feature 917..933 /label=lac O1 promoter 934..939 /label=-10 misc_feature 892..945 /label=BBa_R0011 promoter 892..945 /label=BBa_R0011 misc_feature 955..1424 /label=BBa_K737048 promoter 1433..1467 /label=BBa_J23106 misc_feature 1480..1483 /label=conserved RBS 1476..1487 /label=BBa_B0034 misc_feature 2583..2621 /label=LVA CDS 1494..2621 /label=lacI-LVA misc_feature 1494..2621 /label=BBa_C0012 misc_feature 2622..2646 /label=Help:Barcodes CDS 1494..2621 /label=BBa_C0012 stem_loop 2666..2709 /label=stem_loop misc_feature 2655..2734 /label=BBa_B0010 terminator 2655..2734 /label=BBa_B0010 stem_loop 2750..2769 /label=T7 TE misc_feature 2776..2776 /label=stop polyA_site 2770..2783 /label=polya misc_feature 2743..2783 /label=BBa_B0012 terminator 2743..2783 /label=BBa_B0012 misc_feature 1476..2783 /label=BBa_P0412 misc_feature 2792..2810 /label=TetR 1 misc_feature 2792..2845 /label=BBa_R0040 promoter 2811..2816 /label=-35 misc_feature 2817..2835 /label=TetR 2 promoter 2834..2839 /label=-10 promoter 2792..2845 /label=BBa_R0040 misc_feature 2854..2962 /label=BBa_K737058 ORIGIN 1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta 61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gaattgtgag 901 cggataacaa ttgacattgt gagcggataa caagatactg agcacatact agagccaaga 961 tagtcaatga gacagggcat ctcgcaatct atggcaaaca tcacttcagt tctttctcat 1021 cgggtgatga aaacgcactt cagtctgaaa ggaatatgaa aatgagatca acagacattc 1081 tattttatga ctctgggtaa aatggattga gtaagtgata tagcttacga acattcaaat 1141 caattaaaca tcagaagaga ttttatactc aggtatttaa tctggatctc tgtttattta 1201 aataatgtga aaagagattt ttcacaggag accttataca aaaaaatata aaatacagct 1261 accggttgcc aaagacacta taagcctggc aaaaaaatat tacacaacat aaatgctaat 1321 tgtttatgcg ggctttgtat tgctttctgt atcctacaaa tgagtgaaat ttatgaaaaa 1381 ggctaaaata ctttctggcg tattattact gtgcttttcg tccctactag agtttacggc 1441 tagctcagtc ctaggtatag tgctagctac tagagaaaga ggagaaatac tagatggtga 1501 atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg 1561 tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag 1621 cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac 1681 agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg 1741 tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag 1801 aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca 1861 gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct 1921 gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta 1981 ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc 2041 agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg 2101 gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact 2161 ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca 2221 ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt 2281 ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct 2341 catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca 2401 gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc 2461 ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc 2521 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 2581 aggctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag tgctagtgta 2641 gatctctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 2701 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 2761 gggtgggcct ttctgcgttt atatactaga gtccctatca gtgatagaga ttgacatccc 2821 tatcagtgat agagatactg agcactacta gaggtagggt acagaggtaa gatgttctat 2881 ctttcagacc ttttacttca cgtaatcgga tttggctgaa tattttagcc gccccagtca 2941 gtaatgactg gggcgttttt ta //