##gff-version 3
##sequence-region BBa_K737055 1 2962
BBa_K737055	.	mature_transcript_region	955	1424	.	+	0	ID=BBa_K737048;Name=BBa_K737048
BBa_K737055	.	promoter	2792	2845	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K737055	.	engineered_region	2792	2845	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K737055	.	promoter	2811	2816	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K737055	.	promoter	2834	2839	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K737055	.	non_covalent_binding_site	2792	2810	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K737055	.	non_covalent_binding_site	2817	2835	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K737055	.	promoter	1433	1467	.	+	0	ID=BBa_J23106;Name=BBa_J23106
BBa_K737055	.	engineered_region	1476	2783	.	+	0	ID=BBa_P0412;Name=BBa_P0412
BBa_K737055	.	terminator	2743	2783	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0412
BBa_K737055	.	polyA_site	2770	2783	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K737055	.	engineered_region	2743	2783	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K737055	.	stem_loop	2750	2769	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K737055	.	stop_codon	2776	2776	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K737055	.	terminator	2655	2734	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0412
BBa_K737055	.	engineered_region	2655	2734	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K737055	.	stem_loop	2666	2709	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K737055	.	ribosome_entry_site	1476	1487	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0412
BBa_K737055	.	conserved_region	1480	1483	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K737055	.	CDS	1494	2621	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0412
BBa_K737055	.	CDS	1494	2621	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K737055	.	engineered_tag	2622	2646	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K737055	.	tag	2583	2621	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K737055	.	engineered_region	1494	2621	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K737055	.	promoter	1	35	.	+	0	ID=BBa_J23106;Name=BBa_J23106
BBa_K737055	.	engineered_region	44	883	.	+	0	ID=BBa_P0440;Name=BBa_P0440
BBa_K737055	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K737055	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K737055	.	terminator	843	883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K737055	.	polyA_site	870	883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K737055	.	engineered_region	843	883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K737055	.	stem_loop	850	869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K737055	.	stop_codon	876	876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K737055	.	terminator	755	834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K737055	.	engineered_region	755	834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K737055	.	stem_loop	766	809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K737055	.	CDS	62	721	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K737055	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K737055	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K737055	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K737055	.	mature_transcript_region	2854	2962	.	+	0	ID=BBa_K737058;Name=BBa_K737058
BBa_K737055	.	promoter	892	945	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K737055	.	promoter	911	916	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K737055	.	operator	894	910	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K737055	.	operator	917	933	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K737055	.	engineered_region	892	945	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K737055	.	promoter	934	939	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
>BBa_K737055
tttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacata
ctagagccaagatagtcaatgagacagggcatctcgcaatctatggcaaacatcacttcagttctttctcatcgggtga
tgaaaacgcacttcagtctgaaaggaatatgaaaatgagatcaacagacattctattttatgactctgggtaaaatgga
ttgagtaagtgatatagcttacgaacattcaaatcaattaaacatcagaagagattttatactcaggtatttaatctgg
atctctgtttatttaaataatgtgaaaagagatttttcacaggagaccttatacaaaaaaatataaaatacagctaccg
gttgccaaagacactataagcctggcaaaaaaatattacacaacataaatgctaattgtttatgcgggctttgtattgc
tttctgtatcctacaaatgagtgaaatttatgaaaaaggctaaaatactttctggcgtattattactgtgcttttcgtc
cctactagagtttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatggtgaa
tgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccag
gccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtgg
cacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaat
tgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaa
gcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccagg
atgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacag
tattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctg
ttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattc
agccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcat
cgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgc
gttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatca
aacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaa
tcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttg
gccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctt
tagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagca
ctactagaggtagggtacagaggtaagatgttctatctttcagaccttttacttcacgtaatcggatttggctgaatat
tttagccgccccagtcagtaatgactggggcgtttttta